Hematologic, cytochemical, ultrastructural, and molecular findings of <i>Hepatozoon</i>‐infected flat‐headed cats (<i>Prionailurus planiceps</i>)

Salakij, Chaleow; Salakij, Jarernsak; Narkkong, Nual-Anong; Sirinarumitr, Theerapol; Pattanarangsan, Rattapan

doi:10.1111/j.1939-165x.2008.00011.x

Cited by 22 publications

(32 citation statements)

References 8 publications

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“…However, the gamont-like stage of H. felis is very similar in appearance and size to the gamonts of H. felis described in stained blood smears from domestic cats (10·5 ± 0·6 µ m × 4·7 ± 0·8 µ m, Baneth et al 2013), and bigger than those of Hepatozoon spp. from the ocelot ( Leopardus pardalis ) (7·43 × 4·23 µ m), flat-headed cat ( Prionailurus planiceps ) (9·9 ± 1·1 µ m × 4·8 ± 0·6 µ m 2 ) and leopard cat (9·8 ± 0·4 µ m × 5·2 ± 0·4 µ m) (Metzger et al 2008; Salakij et al 2008, 2010). …”

Section: Discussionmentioning

confidence: 99%

“…have been reported in domestic cats and various species of wild cats, and it is assumed that two or more species could be found in felids worldwide (Criado-Fornelio et al 2006; Kubo et al 2010; Pawar et al 2012; Baneth et al 2013; Tateno et al 2013). However, the parasite in cats was also referred to as Hepatozoon -like or Hepatozoon spp., without committing to a certain species, as the studies which described it were based only on morphological or genetic descriptions (Klopfer et al 1973; Baneth et al 1998; Perez et al 2004; Rubini et al 2006; Metzger et al 2008; Ortuño et al 2008; Salakij et al 2008). Thereafter, the organism was finally morphologically and genetically characterized as Hepatozoon felis and recognized as the predominant agent of feline hepatozoonosis (Baneth et al 2013).…”

Section: Introductionmentioning

confidence: 99%

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Hepatozoon silvestris sp. nov.: morphological and molecular characterization of a new species of Hepatozoon (Adeleorina: Hepatozoidae) from the European wild cat (Felis silvestris silvestris)

et al. 2016

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SUMMARYBased on morphological and genetic characteristics, we describe a new species of Hepatozoon in the European wild cat (Felis silvestris silvestris), herein named Hepatozoon silvestris sp. nov. The study also provides the first data on the occurrence of H. felis in this wild felid. Hepatozoon meronts were observed in multiple cross-sections of different organs of four (44%) cats. Additionally, extracellular forms, resembling mature gamonts of Hepatozoon, were found in the spleen and myocardium of two cats. Furthermore, tissues of six animals (67%) were positive by PCR. Hepatozoon felis was identified infecting one cat (11%), whereas the 18S rRNA sequences of the remaining five cats (56%) were identical, but distinct from the sequences of H. felis. Phylogenetic analyses revealed that those sequences form a highly supported clade distant from other Hepatozoon spp. Future studies should include domestic cats from the areas where the wild cats positive for H. silvestris sp. nov. were found, in order to investigate their potential role to serve as intermediate hosts of this newly described species. Identification of its definitive host(s) and experimental transmission studies are required for elucidating the full life cycle of this parasite and the possible alternative routes of its transmission.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hepatozoon silvestris sp. nov.: morphological and molecular characterization of a new species of Hepatozoon (Adeleorina: Hepatozoidae) from the European wild cat (Felis silvestris silvestris)

et al. 2016

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“…A frog Hepatozoon sp. has been detected in flatheaded cats (Prionailurus planiceps) from Thailand, suggesting that frogs may have been the source of infection in this case (Salakij et al 2008).…”

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confidence: 96%

“…Few molecular studies have been done concerning the molecular characterization of Hepatozoon species among domestic and wild felids (Criado-Fornelio et al 2006;Rubini et al 2006;Metzger et al 2008;Jittapalapong et al 2006;Ortuño et al 2007;Salakij et al 2008;André et al 2010). The present work aimed to characterize molecularly the presence of Hepatozoon sp.…”

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confidence: 97%

Molecular characterization of Hepatozoon sp. in cats from São Luís Island, Maranhão, Northeastern Brazil

et al. 2011

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Few molecular studies have been done concerning the molecular characterization of Hepatozoon species among domestic and wild felids. The present work aimed to characterize molecularly the presence of Hepatozoon sp. DNA in cat blood samples from São Luís Island, Maranhão state, Northeastern Brazil. EDTA-whole blood samples were collected from 200 domestic cats with outdoor and wood areas access from São Luís, Maranhão, Brazil. Each sample of extracted DNA was used as a template in PCR reactions aiming to amplify a partial sequence of 18S rRNA of Hepatozoon spp. We also performed sequence alignment to establish the identity of the parasite species infecting these animals using DNA sequences based on 18S rRNA. From 200 sampled cats, Hepatozoon DNA was only found in one animal (0.5%). The found Hepatozoon DNA showed 97% of identity with Hemobartonella felis isolates 1 and 2 from Spain. When analyzing the phylogenetic tree, the found Hepatozoon DNA was in the same clade than H. felis isolates. Our findings suggest that more than one species of Hepatozoon could infect felids in Brazil.

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“…During the last 30 years, the use of blood profiles to estimate the welfare of wild cats in both captive and wild settings has become a usual practice among zoo veterinaries and field zoologists. At present, for 17 of the 38 wild cat species blood profiles have been obtained and described in scientific literature (Akuzawa, Mochizuki, & Yasuda, ; Brown, Lappin, Brown, Munkhtsog, & Swanson, ; Caro et al., ; Dunbar, Nol, & Linda, ; Erasmus, ; Kocan, Blouin, & Glenn, ; Marco, Martinez, Pastor, & Lavin, ; Prihirunkit, Salakij, Apibal, & Narkkong, ; Salakij, Salakij, Narkkong, Sirinarumitr, & Pattanarangsan, ,b, b, , , and others). However, blood profiles can be influenced significantly by the sampling procedure, including capture method, type of anesthesia, and time to blood collection (Moen, Rasmussen, Burdett, & Pelican, ; Serieys et al., ).…”

Section: Introductionmentioning

confidence: 99%

The method matters: The effect of handling time on cortisol level and blood parameters in wild cats

et al. 2018

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Blood analysis has recently become a popular tool to assess the welfare of the wild cats. However, the estimates of blood parameters may depend on the sampling method. We have tested (1) if the sampling procedure influences blood parameters and (2) what parameters are the most efficient in assessing the physiological status in wild cat species. We assessed the effect of handling time on red blood cells (RBC) and white blood cells (WBC) counts, the ratio of neutrophils to lymphocytes (N/L ratio), and serum cortisol level within 1 hr after the capture of the animal in six far-east wild cats (Prionailurus bengalensis euptilura). Also, we analyzed literature data in 17 cat species to assess the effect of place of study, type of immobilization, and handling time on WBC count and N/L ratio. Serum cortisol level varied significantly with the handling time. RBC and WBC counts were strongly affected by the handling time. N/L ratio was very robust and did not depend on the handling time. However, the analysis of literature data has shown that the prolonged handling time (over 1 hr) and the type of immobilization significantly influence the N/L ratio, whereas the WBC count does not depend on any of considered factors. We conclude that while most blood parameters of cats are affected by routine handling time, the N/L ratio does not vary if the samples are collected within 1 hr after the capture of the animal. All other tested parameters should be treated with caution.

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Hematologic, cytochemical, ultrastructural, and molecular findings of Hepatozoon‐infected flat‐headed cats (Prionailurus planiceps)

Abstract: These results add to our understanding of hematologic values and blood cell morphology in Hepatozoon-infected flat-headed cats as well as the molecular analysis of the Hepatozoon organism, and may be useful for the health management and evaluation of hemoparasitic disease in this species.

Cited by 22 publications

References 8 publications

Hepatozoon silvestris sp. nov.: morphological and molecular characterization of a new species of Hepatozoon (Adeleorina: Hepatozoidae) from the European wild cat (Felis silvestris silvestris)

Hepatozoon silvestris sp. nov.: morphological and molecular characterization of a new species of Hepatozoon (Adeleorina: Hepatozoidae) from the European wild cat (Felis silvestris silvestris)

Molecular characterization of Hepatozoon sp. in cats from São Luís Island, Maranhão, Northeastern Brazil

The method matters: The effect of handling time on cortisol level and blood parameters in wild cats

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