Hematocrit effect on dried blood spots in adults: a computational study and theoretical considerations

Daousani, Chrysa; Karalis, Vangelis; Malenović, Anđelija; Dotsikas, Yannis

doi:10.1080/00365513.2019.1622033

Cited by 22 publications

(29 citation statements)

References 42 publications

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“…Of note, previous attempts at analyzing blood spots demonstrated significant issues in the quantification. , This is mainly due to the sampling techniques used for collecting blood and the use of small punches from the larger blood spot. This sampling method is subject to variability if metal concentrations vary across the blood spot as can occur because of the hematocrit effect, which is related to the drying pattern of blood on the card. , While new approaches for blood spot collection and making punches could improve on this problem, − the EDXRF largely avoids this problem by measuring essentially the whole blood spot, and the beam diameter can be adjusted to further aid with this issue. Furthermore, EDXRF measurements have the great benefit of being nondestructive, so the blood spots can still be retained for other analyses.…”

Section: Discussionmentioning

confidence: 99%

“…This has been used in lead screening as an alternative approach for measuring venous blood lead levels. − The benefit of using a filter paper is that the samples can be collected in the field, stored at ambient temperatures, and then analyzed at a later date. However, the most frequently used analysis methods require some form of digestion of a small punch from the blood spot or laser ablation to quantify the metal in the small part of the blood spot. ,, These sampling techniques face challenges such as the hematocrit level influencing blood wicking and drying and therefore potentially causing variation in the metal concentration depending on where in the spot is sampled. , …”

Section: Introductionmentioning

confidence: 99%

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Feasibility of Lead Exposure Assessment in Blood Spots using Energy-Dispersive X-ray Fluorescence

Specht

Obrycki

Mazumdar

et al. 2021

Environ. Sci. Technol.

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Collecting blood spots from newborns is a common procedure used to diagnose multiple health conditions. Fingerstick blood samples are routinely collected from children to diagnose elevated blood lead levels. In our study, we wanted to test the feasibility of using a high-power energy-dispersive X-ray fluorescence (EDXRF) device to accurately measure the concentration of lead in blood spots. We created spotted standards of a known concentration of lead on a filter paper at different volumes and concentrations. We determined the detection limit for lead through repeated measurements of our standards and calibration line slopes. We also tested the variability of the measured lead concentration across procedures and spotted blood volumes and found no significant additions to uncertainty in measurements. Finally, we compared blood lead concentrations measured by EDXRF and atomic absorption spectroscopy (AAS) and found EDXRF to be a significant predictor of blood lead (n = 22, R = 0.98, p value <0.001) with an average detection limit of 1.7 μg/dL of blood lead. This detection limit is similar to that of the AAS technique, which is commonly used in clinical testing laboratories for blood lead surveillance. These findings provide a proof of concept that blood spots measured by EDXRF may be used as a surveillance tool for lead exposure, even at elevated blood lead levels of 2–3 μg/dL.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Feasibility of Lead Exposure Assessment in Blood Spots using Energy-Dispersive X-ray Fluorescence

Specht

Obrycki

Mazumdar

et al. 2021

Environ. Sci. Technol.

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“…It would be useful, if the influence of drugs (with various physicochemical properties) and their effect on spreading of the DBS on a card, was further assessed. The effect of Hct on DBS was evaluated in a computational study by Daousani et al [76]. The aim of the research was the suggestion of a Hct effect correction, which could be further implemented in DBS quantitative analysis, in case a DBS partial spot is analyzed.…”

Section: Mathematical and Computational Approaches For The Correction Of Hct Effectmentioning

confidence: 99%

The Evolving Role of Microsampling in Therapeutic Drug Monitoring of Monoclonal Antibodies in Inflammatory Diseases

et al. 2021

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Monoclonal antibodies (mAbs) have been extensively developed over the past few years, for the treatment of various inflammatory diseases. They are large molecules characterized by complex pharmacokinetic and pharmacodynamic properties. Therapeutic drug monitoring (TDM) is routinely implemented in the therapy with mAbs, to monitor patients’ treatment response and to further guide dose adjustments. Serum has been the matrix of choice in the TDM of mAbs and its sampling requires the visit of the patients to laboratories that are not always easily accessible. Therefore, dried blood spots (DBS) and various microsampling techniques have been suggested as an alternative. DBS is a sampling technique in which capillary blood is deposited on a special filter paper. It is a relatively simple procedure, and the patients can perform the home-sampling. The convenience it offers has enabled its use in the quantification of small-molecule drugs, whilst in the recent years, studies aimed to develop microsampling methods that will facilitate the TDM of mAbs. Nevertheless, hematocrit still remains an obstacle that hinders a more widespread implementation of DBS in clinical practice. The introduction of novel analytical techniques and contemporary microsampling devices can be considered the steppingstone to the attempts made addressing this issue.

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“…; DÉGLON; LEUTHOLD;THOMAS, 2015;MAURER, 2017A;DAOUSANI et al, 2019;FREY, DAMON & BADU-TAWIAH, 2019;LUGINBÜHL et al, 2019).Em consequência da necessidade de coleta de pouca quantidade de amostra, o DBS minimiza a manipulação de material biológico e permite que a mesma seja enviada por correio em condições de temperatura ambiente, diminuindo o risco de quebra e transmissão de doenças. Dessa forma, ele se caracteriza como uma análise de baixo risco biológico(STOVE et al, 2012;CARVALHO et al, 2019).…”

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Detecção de esteroides anabólicos androgênicos e seus produtos de biotransformação em dried urine spots por ionização por paper spray acoplada à espectrometria de massas (PS-MS)

Linhares¹

2020

Revista CML

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Hematocrit effect on dried blood spots in adults: a computational study and theoretical considerations

Cited by 22 publications

References 42 publications

Feasibility of Lead Exposure Assessment in Blood Spots using Energy-Dispersive X-ray Fluorescence

Feasibility of Lead Exposure Assessment in Blood Spots using Energy-Dispersive X-ray Fluorescence

The Evolving Role of Microsampling in Therapeutic Drug Monitoring of Monoclonal Antibodies in Inflammatory Diseases

Detecção de esteroides anabólicos androgênicos e seus produtos de biotransformação em dried urine spots por ionização por paper spray acoplada à espectrometria de massas (PS-MS)

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