Hemato-biochemical responses and histopathological alterations in the gill and kidney tissues of Osteobrama belangeri (Valenciennes, 1844) exposed to different sub-lethal unionized ammonia

Mangang, Yumnam Abungcha; Pandey, Pramod Kumar

doi:10.1016/j.aquaculture.2021.736887

Cited by 25 publications

(12 citation statements)

References 53 publications

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“…Furthermore, ammonia, the main product of nitrogen metabolism, typically causes stress-induced alterations in fish gills if ammonia accumulates to a level that exceeds the threshold tolerated by fish [ 46 ]. The responses of the gills and variations in their condition are easily identified and provide valuable detail that reflects the health conditions of fish [ 47 ].…”

Section: Resultsmentioning

confidence: 99%

Effects of Melissa officinalis L. Essential Oil in Comparison with Anaesthetics on Gill Tissue Damage, Liver Metabolism and Immune Parameters in Sea Bass (Lateolabrax maculatus) during Simulated Live Transport

Wang

Mei

Cao

et al. 2021

Biology

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In the current study, Melissa officinalis L. essential oil (MOEO), a novel sedative and anaesthetic, was employed in transport water to obtain a lower stress effect and higher survival rate for live marine fish. The effect of MOEO and various types of anaesthetics, administered at a low temperature on gill morphology, liver function and immunological parameters of living sea bass (Lateolabrax maculatus) subjected to transport stress, was evaluated to optimize the anaesthetic and sedative concentrations during live sea bass transport. Light microscopy and scanning electron microscopy of sea bass, subjected to simulated live transport for 72 h, demonstrated that the changes in the morphological characteristics of gill tissue treated with 40 mg/L MOEO (A3 group) were minimal in comparison to those observed in untreated sea bass. The results of pyruvate kinase (PK), phosphofructokinase (PFK), hexokinase (HK), hepatic glycogen (Gly), superoxide dismutase (SOD), lipid peroxides (MDA) and Caspase-3 assays indicated that the glycolysis rate, energy consumption, lipid peroxidation and hepatocyte apoptosis were the lowest in the A3 group. The values of the two immune parameters, lysozyme (LZM) and fish immunoglobulin M (IgM), indicated the strongest immunity ability in the A3 group. After 12 h recovery, sea bass treated with 30 mg/L MS-222 (B group) displayed a 100% survival rate, sea bass treated with 20 mg/L (A2 group) and 40 mg/L (A3 group) MOEO displayed a 96% survival rate, sea bass treated with 20 mg/L eugenol (C group) had a 94% survival rate, and untreated sea bass (CK group) had a 50% survival rate. Therefore, the addition MOEO to the transport water had anaesthetic and sedative effects similar to MS-222 and eugenol. The results confirmed that the addition of MOEO to the transport water could reduce tissue damage, energy metabolism, and the oxidative stress response in sea bass during transport.

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Section: Resultsmentioning

confidence: 99%

Effects of Melissa officinalis L. Essential Oil in Comparison with Anaesthetics on Gill Tissue Damage, Liver Metabolism and Immune Parameters in Sea Bass (Lateolabrax maculatus) during Simulated Live Transport

Wang

Mei

Cao

et al. 2021

Biology

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“…It is well known that gills are the first organ to react to adverse environmental conditions. Some studies have shown that acute ammonia exposure can cause osmotic dysfunction and severe histopathological damage to the gills, which in turn may compromise gas exchange and ion and acid–base regulation (Gao et al, 2021; Mangang & Pandey, 2021). In the present study, histological alterations of the gills, such as cell hyperplasia, epithelial lifting, fusion of the secondary lamellae, blood congestion with hemorrhage, and destruction of lamellar epithelium, were observed.…”

Section: Discussionmentioning

confidence: 99%

Determination of acute toxicity of unionized ammonia in juvenile longfin yellowtail (Seriola rivoliana)

Vera

Galarza

Reinoso

et al. 2023

J World Aquaculture Soc

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The median lethal concentration (LC50) of unionized ammonia (NH3‐N) in longfin yellowtail, Seriola rivoliana juveniles was assessed after 96 h of exposure using a semistatic water system. Experimental fish were exposed in triplicate to different ammonia concentrations: 0.55 ± 0.00; 0.94 ± 0.02; 1.18 ± 0.00; 1.72 ± 0.02, and 1.97 ± 0.09 NH3‐N mg/L. Additionally, a control group (0.00 ± 0.00 NH3‐N mg/L) was included. The 96 h LC50 of unionized ammonia in S. rivoliana was 0.58 mg/L. Fish exposed to different ammonia concentrations for 96 h displayed several lesions on gill tissues, that is, hyperplasia, epithelial lifting, fusion of the secondary lamellae, and some irreversible damage. Erratic behavior, such as swimming in circles and hyperventilation was observed before the fish died. The safe concentration of unionized ammonia in this study was estimated at 0.06 and 1.68 mg/L of total ammonia nitrogen (TAN). The outcomes observed in this study provide valuable insights into unionized ammonia effects for better water quality management in semi‐intensive and intensive systems for S. rivoliana culture.

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“…In particular, the gill functions as the main organ for gas exchange, making it more easily attacked by ammonia exposure [ 4 ]. Ammonia exposure caused hyperplasia of primary lamellae epithelium and edema of secondary lamellae epithelium both in the model zebrafish [ 5 ] and Osteobrama belangeri [ 6 ]. In golden pompano ( Trachinotus ovatus ), gill oxidative damage caused by acute ammonia stress was proved to be regulated by the hypoxia-inducible factor 1α (HIF-1α)/nuclear factor kappa-B (NF-κb) signaling pathway [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Liver Injury and Metabolic Dysregulation in Largemouth Bass (Micropterus salmoides) after Ammonia Exposure

Zou

Wang

et al. 2023

Metabolites

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Elevated environmental ammonia leads to respiratory disorders and metabolic dysfunction in most fish species, and the majority of research has concentrated on fish behavior and gill function. Prior studies have rarely shown the molecular mechanism of the largemouth bass hepatic response to ammonia loading. In this experiment, 120 largemouth bass were exposed to total ammonia nitrogen of 0 mg/L or 13 mg/L for 3 and 7 days, respectively. Histological study indicated that ammonia exposure severely damaged fish liver structure, accompanied by increased serum alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase activity. RT-qPCR results showed that ammonia exposure down-regulated the expression of genes involved in glycogen metabolism, tricarboxylic acid cycle, lipid metabolism, and urea cycle pathways, whereas it up-regulated the expression of genes involved in gluconeogenesis and glutamine synthesis pathways. Thus, ammonia was mainly converted to glutamine in the largemouth bass liver during ammonia stress, which was rarely further used for urea synthesis. Additionally, transcriptome results showed that ammonia exposure also led to the up-regulation of the oxidative phosphorylation pathway and down-regulation of the mitogen-activated protein kinase signaling pathway in the liver of largemouth bass. It is possible that the energy supply of oxidative phosphorylation in the largemouth bass liver was increased during ammonia exposure, which was mediated by the MAPK signaling pathway.

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Hemato-biochemical responses and histopathological alterations in the gill and kidney tissues of Osteobrama belangeri (Valenciennes, 1844) exposed to different sub-lethal unionized ammonia

Cited by 25 publications

References 53 publications

Effects of Melissa officinalis L. Essential Oil in Comparison with Anaesthetics on Gill Tissue Damage, Liver Metabolism and Immune Parameters in Sea Bass (Lateolabrax maculatus) during Simulated Live Transport

Effects of Melissa officinalis L. Essential Oil in Comparison with Anaesthetics on Gill Tissue Damage, Liver Metabolism and Immune Parameters in Sea Bass (Lateolabrax maculatus) during Simulated Live Transport

Determination of acute toxicity of unionized ammonia in juvenile longfin yellowtail (Seriola rivoliana)

Liver Injury and Metabolic Dysregulation in Largemouth Bass (Micropterus salmoides) after Ammonia Exposure

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