Hemangioblast, hemogenic endothelium, and primitive versus definitive hematopoiesis

Lacaud, Georges; Kouskoff, Valérie

doi:10.1016/j.exphem.2016.12.009

Cited by 105 publications

(97 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Hemogenic angioblasts can give rise to primitive hematopoietic cells in the yolk sac, and hemogenic endothelial cells can generate HSCs in the DA (Gritz and Hirschi, 2016;Lacaud and Kouskoff, 2017).…”

Section: Discussionmentioning

confidence: 99%

Dual Role of Jam3b in Early Hematopoietic and Vascular Development

Kobayashi

Kobayashi-Sun

Hirakawa

et al. 2019

Preprint

View full text Add to dashboard Cite

In order to efficiently derive hematopoietic stem cells (HSCs) from pluripotent precursors, it is crucial to understand how mesodermal cells acquire hematopoietic or endothelial identity due to their close developmental connection. Although Npas4 has been recently identified as a conserved master regulator of hemato-vascular development, the molecular mechanisms underlying the cell fate divergence between hematopoietic and vascular endothelial cells are still unclear. Here, we show in zebrafish that the divergence of hematopoietic and vascular endothelial cells in mesodermal cells is regulated by Junctional adhesion molecule 3b (Jam3b) via two independent signaling pathways.Mutation of jam3b led to the reduction of npas4l expression in the posterior lateral plate mesoderm and defect of both hematopoietic and vascular development. Mechanistically, we uncover that Jam3b promotes endothelial specification by regulating npas4l expression through the repression of the Rap1a-Erk signaling cascade. Jam3b subsequently promotes hematopoietic development including HSCs by regulating lrrc15 expression in endothelial precursors through the activation of an integrindependent signaling cascade. Our data provide insight into the divergent mechanisms for instructing hematopoietic or vascular fates from mesodermal cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Dual Role of Jam3b in Early Hematopoietic and Vascular Development

Kobayashi

Kobayashi-Sun

Hirakawa

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…One study was able to regulate the WNT–βcatenin and Nodal–activin pathways during EB formation to produce an hESC/iPSC derived CD34 + CD43 − population that were thought to represent a “definitive” hematopoietic progenitor cell capable of T cell, erythroid and myeloid potential while characterizing an hESC/iPSC derived CD34 + CD43 + population as the “primitive” progenitor cells that can only produce restricted erythroid, macrophage, and megakaryocytic lineages . However, it is likely these CD34 + CD43 − hESC/iPSC‐derived cells still represent a primitive hematopoietic lineage, possibly similar to a second wave of hematopoietic cells that develop in the yolk sac during early embryogenesis . Indeed, these hESC/hiPSC‐derived CD34 + CD43 − cells cultured on OP9‐DL1 were able to produce CD3 + TCRαβ T cells but failed to mature to functional CD4 + or CD8 + T cells .…”

Section: Making T Cells In a Dishmentioning

confidence: 99%

Concise Review: Human Pluripotent Stem Cells to Produce Cell-Based Cancer Immunotherapy

Huang

Lai

et al. 2018

Stem Cells

View full text Add to dashboard Cite

Human pluripotent stem cells (PSCs) provide a promising resource to produce immune cells for adoptive cellular immunotherapy to better treat and potentially cure otherwise lethal cancers. Cytotoxic T cells and natural killer (NK) cells can now be routinely produced from human PSCs. These PSC-derived lymphocytes have phenotype and function similar to primary lymphocytes isolated from peripheral blood. PSC-derived T and NK cells have advantages compared with primary immune cells, as they can be precisely engineered to introduce improved anti-tumor activity and produced in essentially unlimited numbers. STEM CELLS 2018;36:134-145 SIGNIFICANCE STATEMENTHuman pluripotent stem cells provide an important strategy to produce immune cells that can better target and treat refractory cancers.

show abstract

“…Recently, it has been proposed that blood cells precursors can also be produced by hemogenic angioblasts. Therefore, it is possible that scattered, blood-vessel-independent hemogenic progenitors may also exist (Tanaka et al 2014; Jankowska-Steifer et al 2015; Lacaud and Kouskoff 2017; Zamir et al 2017). …”

Section: Introductionmentioning

confidence: 99%

Cells with hematopoietic potential reside within mouse proepicardium

Jankowska‐Steifer

Niderla-Bielińska

Ciszek

et al. 2018

Histochem Cell Biol

View full text Add to dashboard Cite

During embryonic development, hematopoietic cells are present in areas of blood-vessel differentiation. These hematopoietic cells emerge from a specific subpopulation of endothelial cells called the hemogenic endothelium. We have previously found that mouse proepicardium contained its own population of endothelial cells forming a network of vascular tubules. We hypothesize that this EC population contains cells of hematopoietic potential. Therefore, we investigated an in vitro hematopoietic potential of proepicardial cell populations. The CD31+/CD45−/CD71− cell population cultured for 10 days in MethocultTM gave numerous colonies of CFU-GEMM, CFU-GM, and CFU-E type. These colonies consisted of various cell types. Flk-1+/CD31−/CD45−/CD71−, and CD45+ and/or CD71+ cell populations produced CFU-GEMM and CFU-GM, or CFU-GM and CFU-E colonies, respectively. Immunohistochemical evaluations of smears prepared from colonies revealed the presence of cells of different hematopoietic lineages. These cells were characterized by labeling with various combinations of antibodies directed against CD31, CD41, CD71, c-kit, Mpl, Fli1, Gata-2, and Zeb1 markers. Furthermore, we found that proepicardium-specific marker WT1 co-localized with Runx1 and Zeb1 and that single endothelial cells bearing CD31 molecule expressed Runx1 in the proepicardial area of embryonic tissue sections. We have shown that cells of endothelial and/or hematopoietic phenotypes isolated from mouse proepicardium possess hematopoietic potential in vitro and in situ. These results are supported by RT-PCR analyses of proepicardial extract, which revealed the expression of mRNA for crucial regulatory factors for hemogenic endothelium specification, i.e., Runx1, Notch1, Gata2, and Sox17. Our data are in line with previous observation on hemangioblast derivation from the quail PE.Electronic supplementary materialThe online version of this article (10.1007/s00418-018-1661-1) contains supplementary material, which is available to authorized users.

show abstract

Hemangioblast, hemogenic endothelium, and primitive versus definitive hematopoiesis

Cited by 105 publications

References 61 publications

Dual Role of Jam3b in Early Hematopoietic and Vascular Development

Dual Role of Jam3b in Early Hematopoietic and Vascular Development

Concise Review: Human Pluripotent Stem Cells to Produce Cell-Based Cancer Immunotherapy

Cells with hematopoietic potential reside within mouse proepicardium

Contact Info

Product

Resources

About