Hemagglutination activities of group B, C, D, and G streptococci: demonstration of novel sugar-specific cell-binding activities in Streptococcus suis

Kurl, Daya N.; Haataja, Sauli; Finne, Jukka

doi:10.1128/iai.57.2.384-389.1989

Cited by 35 publications

(9 citation statements)

References 31 publications

(36 reference statements)

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“…None of the field isolates recovered from lungs (cases of pneumonia) and only 50% of the field isolates recovered from several tissues (cases of septicemia) were positive ( Table 2). This is in agreement with previous information (8). Hemagglutination was observed with microorganisms grown either on bovine blood agar plates or in Todd-Hewitt broth and was not inhibited by the addition of D-mannose.…”

Section: Gottschalk Et Al Submitted For Publication)supporting

confidence: 93%

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Hemagglutination properties of Streptococcus suis

et al. 1990

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A total of 49 strains (23 reference strains and 26 field isolates) of Streptococcus suis were tested for their ability to agglutinate erythrocytes from different animal species. Ten different hemagglutination patterns were established. Thirty-three strains (67%) did not agglutinate any of the erythrocytes tested; sixteen strains (33%) agglutinated erythrocytes from one or more animal species. Different strains belonging to the same capsular type presented different hemagglutination patterns. No correlation was found between the tissue origin and/or the virulence (evaluated in 4-week-old mice) of different field isolates and their hemagglutination activity. Hydrophobic surface properties were also evaluated. All S. suis strains studied appeared to possess a hydrophilic cell surface. Morphologically similar fimbriae were observed on hemagglutinating as well as on nonhemagglutinating strains of S. suis. This study provides evidence that certain strains of S. suis possess hemagglutinating properties which do not appear to involve hydrophobic interactions. The possible role of fimbriae in hemagglutination remains unclear.

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Section: Gottschalk Et Al Submitted For Publication)supporting

confidence: 93%

“…In most cases, the hemagglutination activity was temperature independent. These results are at variance with a previous work (8) in which hemagglutination was stronger at 0°C than at 220C. The cell surface hydrophobicity was measured by using the salt aggregation test (9).…”

Section: Gottschalk Et Al Submitted For Publication)supporting

confidence: 63%

Hemagglutination properties of Streptococcus suis

et al. 1990

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“…Heating the samples at 45ºC, 60ºC and 100ºC for 30 min assessed the heat-stability of culture supernatants. The effect of proteolysis on activity was examined by treating the samples with trypsin, protease K and pepsin (100 μg/mL each) at 37ºC for 60 min (18). Residual hemolytic activity was measured on microplates as described above.…”

Section: Physico-chemical Properties Of the Culture Filtratesmentioning

confidence: 99%

Virulence-associated characteristics of Enterococcus faecalis strains isolated from clinical sources

Furumura

Figueiredo

Carbonell

et al. 2006

Braz. J. Microbiol.

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Thirty-two clinical isolates of Enterococcus faecalis were screened for virulence factors. Twenty-four (75%) isolates produced hemolysin on Mueller-Hinton blood agar plates with sheep erythrocytes. However, the cell free heat-stable hemolysin was detected in all isolates (100%) of E. faecalis when grown in BHI-GA (BHI medium supplemented with 1% glucose and 0.03% L-arginine), but not in BHI broth alone. Twenty-four isolates (75%) produced caseinase and 23 (71.9%) lipase, but none of the isolates produced gelatinase. Fifteen (46.9%) culture filtrates caused rounding and membrane alterations with blebbing formation followed by death in HeLa and HEp-2 cells, but not in Vero cells. Thirteen isolates (40.6%) agglutinated rabbit erythrocytes, but did not produce hemagglutination in other bloods, containing or not 1% D-manose. Sixteen (50%) E. faecalis isolates adhered to HeLa cells and thirteen (40.6%) to HEp-2 cells, but all isolates adhered to polypropylene microtiter plates, indicating that clinical E. faecalis possess the ability to form biofilm in vitro. All the isolates were resistant to the bactericidal action of normal serum and did not produce aerobactin. These findings suggest that adherence and consequently biofilm formation on ephitelial host cells are the first steps in the E. faecalis virulence and that hemolysin, lipase, caseinase and other virulence factors act as causative of human epithelial cell damages.

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“…Non-specific physicochemical interactions might be mediated by superficial, hydrophobic structures (DOYLE and ROSENBERG, 1990). A specific adhesin on a bacterial surface is often correlated with haemagglutinating properties, this relationship has been observed for various bacteria (HOSCHUTZKY et al, 1989;KURL et al, 1989;KANOE et al, 1992; DESLAURIERS and MOUTON, 1992).…”

Section: Introductionmentioning

confidence: 83%

Relationship between Haemagglutination and HeLa-cell Adherence of Rhodococcus equi

Bern

Lämmler

1996

Journal of Veterinary Medicine, Series B

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Summary In this study, 45 Rhodococcus equi isolates from animals and humans were identified by the Api Coryne system, and serologically with monospecific antisera against capsular types 1–7, with serotypes 1 and 2 predominating. Regardless of serotype, 14 out of 31 isolates from animals and one of 14 isolates from humans expressed 15–17‐kD virulence‐associated proteins. In hexadecane adherence studies, serotype 2 isolates generally displayed hydrophobic surfaces. In addition, isolates of serotype 1 generally haemagglutinated erythrocytes from rabbits. Neither property appeared to be related to the occurrence of the virulence‐associated proteins. By contrast, the haemagglutination reaction correlated significantly with the adherence of the bacteria to HeLa cells. This adhesion might serve as additional marker of virulence among isolates of R. equi and might be useful in epidemiological studies.

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Hemagglutination activities of group B, C, D, and G streptococci: demonstration of novel sugar-specific cell-binding activities in Streptococcus suis

Cited by 35 publications

References 31 publications

Hemagglutination properties of Streptococcus suis

Hemagglutination properties of Streptococcus suis

Virulence-associated characteristics of Enterococcus faecalis strains isolated from clinical sources

Relationship between Haemagglutination and HeLa-cell Adherence of Rhodococcus equi

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