Helper bacteria halt and disarm mushroom pathogens by linearizing structurally diverse cyclolipopeptides

Hermenau, Ron; Kugel, Susann; Komor, Anna J.; Hertweck, Christian

doi:10.1073/pnas.2006109117

Cited by 31 publications

(18 citation statements)

References 34 publications

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“…Tolaasin, sessilin, and viscosin are well-characterized non-ribosomal peptides with antifungal activity. The tolaasin was first characterized in the fungal pathogen Pseudomonas tolaasii which is the causative agent of the Brown blotch disease, while viscosin and sessilin have been found to be effective against Aspergillus fumigatus , Batrachochytrium dendrobatidis, Rhizoctonia solani, and Pythium myriotylum [ 30 , 31 ].…”

Section: Resultsmentioning

confidence: 99%

Potential Biocontrol Activities of Populus Endophytes against Several Plant Pathogens Using Different Inhibitory Mechanisms

et al. 2022

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The plant microbiome can be used to bolster plant defense against abiotic and biotic stresses. Some strains of endophytes, the microorganisms within plants, can directly inhibit the growth of plant fungal pathogens. A previously isolated endophyte from wild Populus (poplar), WPB of the species Burkholderia vietnamiensis, had robust in vitro antifungal activity against pathogen strains that are highly virulent and of concern to Pacific Northwest agriculture: Rhizoctonia solani AG-8, Fusarium culmorum 70110023, and Gaemannomyces graminis var. tritici (Ggt) ARS-A1, as well as activity against the oomycete, Pythium ultimum 217. A direct screening method was developed for isolation of additional anti-fungal endophytes from wild poplar extracts. By challenging pathogens directly with dilute extracts, eleven isolates were found to be inhibitory to at least two plant pathogen strains and were therefore chosen for further characterization. Genomic analysis was conducted to determine if these endophyte strains harbored genes known to be involved in antimicrobial activities. The newly isolated Bacillus strains had gene clusters for production of bacillomycin, fengicyn, and bacillibactin, while the gene cluster for the synthesis of sessilin, viscosin and tolaasin were found in the Pseudomonas strains. The biosynthesis gene cluster for occidiofungin (ocf) was present in the Burkholderia vietnamiensis WPB genome, and an ocf deletion mutant lost inhibitory activity against 3 of the 4 pathogens. The new isolates lacked the gene cluster for occidiofungin implying they employ different modes of action. Other symbiotic traits including nitrogen fixation, phosphate solubilization, and the production of auxins and siderophores were investigated. Although it will be necessary to conduct in vivo tests of the candidates with pathogen-infected agricultural crops, the wild poplar tree microbiome may be a rich source of beneficial endophyte strains with potential for biocontrol applications against a variety of pathogens and utilizing varying modes of action.

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Section: Resultsmentioning

confidence: 99%

Potential Biocontrol Activities of Populus Endophytes against Several Plant Pathogens Using Different Inhibitory Mechanisms

et al. 2022

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“…5 ). A recent study also demonstrated that a lipopeptide of a pathogen ( Pseudomonas tolaasii ) is modified by a “helper” bacterium to protect the mushroom Agaricus bisporus from the pathogen’s virulence factors ( 42 ). Furthermore, lipopeptide modifications are commonly used for niche protection—a prominent trait in soil-dwelling Streptomyces strains against a competing Bacillus sp.…”

Section: Resultsmentioning

confidence: 99%

Lipopeptide-mediated bacterial interaction enables cooperative predator defense

Zhang

Mukherji

Chowdhury

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

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Bacteria are inherently social organisms whose actions should ideally be studied within an interactive ecological context. We show that the exchange and modification of natural products enables two unrelated bacteria to defend themselves against a common predator. Amoebal predation is a major cause of death in soil bacteria and thus it exerts a strong selective pressure to evolve defensive strategies. A systematic analysis of binary combinations of coisolated bacteria revealed strains that were individually susceptible to predation but together killed their predator. This cooperative defense relies on a Pseudomonas species producing syringafactin, a lipopeptide, which induces the production of peptidases in a Paenibacillus strain. These peptidases then degrade the innocuous syringafactin into compounds, which kill the predator. A combination of bioprospecting, coculture experiments, genome modification, and transcriptomics unravel this novel natural product-based defense strategy.

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“…Considering the food importance of mushroom species damaged by the above cited Pseudomonas strains and the consequent heavy economic losses, recently some attempts were made to avoid these severe problems. Some positive results were obtained using helper bacteria belonging to the Mycetocola genus which inactivated tolaasin by hydrolyzing the lactone ring and thus generating a liner peptide [ 142 ]. A different mechanism of tolaasin detoxification seemed to be operated by Microbacterium foliorum , which hydrolyzed the peptide chain in two points [ 143 ].…”

Section: Lipodepsipeptides Produced By Bacteria Pathogens Of Mushroomsmentioning

confidence: 99%

Bioactive Lipodepsipeptides Produced by Bacteria and Fungi

Evidente

2022

IJMS

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Natural products are a vital source for agriculture, medicine, cosmetics and other fields. Lipodepsipeptides (LPDs) are a wide group of natural products distributed among living organisms such as bacteria, fungi, yeasts, virus, insects, plants and marine organisms. They are a group of compounds consisting of a lipid connected to a peptide, which are able to self-assemble into several different structures. They have shown different biological activities such as phytotoxic, antibiotic, antiviral, antiparasitic, antifungal, antibacterial, immunosuppressive, herbicidal, cytotoxic and hemolytic activities. Their biological activities seem to be due to their interactions with the plasma membrane (MP) because they are able to mimic the architecture of the native membranes interacting with their hydrophobic segment. LPDs also have surfactant properties. The review has been focused on the lipodepsipeptides isolated from fungal and bacterial sources, on their biological activity, on the structure–activity relationships of some selected LPD subgroups and on their potential application in agriculture and medicine. The chemical and biological characterization of lipodepsipeptides isolated in the last three decades and findings that resulted from SCI-FINDER research are reported. A critical evaluation of the most recent reviews dealing with the same argument has also been described.

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Helper bacteria halt and disarm mushroom pathogens by linearizing structurally diverse cyclolipopeptides

Cited by 31 publications

References 34 publications

Potential Biocontrol Activities of Populus Endophytes against Several Plant Pathogens Using Different Inhibitory Mechanisms

Potential Biocontrol Activities of Populus Endophytes against Several Plant Pathogens Using Different Inhibitory Mechanisms

Lipopeptide-mediated bacterial interaction enables cooperative predator defense

Bioactive Lipodepsipeptides Produced by Bacteria and Fungi

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