Helium–Neon Laser Irradiation Stimulates Cell Proliferation through Photostimulatory Effects in Mitochondria

Hu, Wan‐Ping; Wang, Jhi-Joung; Yu, Chia-Li; Lan, Cheng‐Che E.; Chen, Gow-Shing; Yu, Hsin‐Su

doi:10.1038/sj.jid.5700826

Cited by 218 publications

(157 citation statements)

References 35 publications

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“…[12][13][14] Finally, these changes will lead to increased expression of growth factors and cell proliferation. 18,19 Furthermore, the most important intracellular organelle that produces reactive oxygen species (ROS) is the mitochondria. ROS is a natural by-product of electron transport chain activity.…”

Section: Introductionmentioning

confidence: 99%

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A Comparative Study of 660 nm Low-Level Laser and Light Emitted Diode in Proliferative Effects of Fibroblast Cells

et al. 2017

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Introduction: In recent years, low-power lasers have been widely used in medicine. With the introduction of affordable light emitted diode (LED), clinical application of LED light has become more and more popular. However, some researchers believe that due to lack of coherence of the LED light, it can be different in terms of biological effects, in comparison with laser. In this study, the biological effects of low-level laser (LLL) to those of LED light are compared and discussed. Methods: Human skin fibroblast cell line Hu02 was irradiated with LLL and LED light with a wavelength of 660 nm, power output of 35 mW and in continuous mode and the control group was not irradiated. The biological effects were compared through analysis of cell proliferation, production of reactive oxygen species (ROS) within the cell and rate of cell division. Results: Our findings showed that production of ROS within the cell was linearly increased both in the LED and laser light irradiated cells. However, laser light is more incremental in comparison to LED light. The MTT results showed that laser light at low energy density (less than 5 J/cm 2 ) increased the rate of cell proliferation after 24 hours. Although, the rate of cell division was increased in energy density of 1 J/cm 2 compared to the control group, this increase was not statistically significant. Discussion: The findings indicated that the coherence properties of laser light provided more energy for the cells, and in a constant energy density, laser light created more oxidative stresses in comparison with LED light.

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Section: Introductionmentioning

confidence: 99%

“…ROS is a natural by-product of electron transport chain activity. LLL was reported to enhance the level of intracellular ROS generation [19][20][21][22] and it has shown that the production of ROS could activate NF-kB pathway. 23 Activation of NFkB effects expression of some genes participating in cell survival and proliferation.…”

Section: Introductionmentioning

confidence: 99%

A Comparative Study of 660 nm Low-Level Laser and Light Emitted Diode in Proliferative Effects of Fibroblast Cells

et al. 2017

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“…Cons equently, the metab olic process es of plants pretreat ed with He-Ne l as er wer e acc eler at ed res ulting i n enhanc ed growth and biomass (Chen et al , 2005). Pr eviousl y r eports sugges t v arious phot ostim ulat or y eff ects of H e -Ne l aser on mitochondria including i ncreas e i n mitochondrial membrane pot ential, c A MP and ATP t hrough enhanc ed c ytochr ome c oxidas e activit y promoti ng phosphor ylati on of J un Nterminal ki nas e (JNK)/ activ at or prot ein -1 (A P-1) expr ession enhancing growt h factor releas e (Hu et al , 2007). He-N e las er radiations increase ATP l ev els (Kar u et al , 1995;H u et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Role of He-Ne laser pre-treatment in protecting Zea mays against the deleterious effects of ultraviolet radiations

Abu-Elsaoud¹,

Shahda²

2017

Egypt. J. Exp. Biol. (Bot.)

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The current st udy aims to r ec ord the rol e of the pretreatment of Z ea m ays grains with a He-Ne las er in d amage rep air of ultr aviolet (UVA+B) radiati on eff ect. Plants wer e divided into f our groups; c ontrol group, group expos ed to t he He -Ne l aser onl y f or 1-300 sec onds, group expos ed t o U V A+ B only and protect ed group. In t he prot ect ed group the grains were tr eated with diff erent H e -Ne las er irradiation dur ation f or two w eeks t hen expos ed to 3. 6 and 7. 2 KJ m − 2 d −1 UVA+B radiation.Irradiation with H e-Ne las er significantl y (p ≤ 0.05) increas ed shoot length, number of l eav es, s hoot biomas s (week 1), root dr y w eight, c hl orophyll -a, chl orophyll -b, and t ot al c hlor ophyll . Sup eroxide free r adical, hydrogen peroxide and lipid peroxidation wer e decreas ed at l ow dos es and increas ed with higher dos es of las er. V arious d elet erious eff ects of ultravi olet r adiations w ere recorded in Zea m ays i ncluding decr eased phot os ynt hetic pigments, s hoot and root biomass, and root and shoot length. The pretreatm ent with a l aser bef ore expos ure t o UVA+B radiation c auses a slight i ncrease i n plant l engt h and t he number of leav es, an increase of r oot length, whil e a decreas e of shoot /root length and an i ncreas e i n l eaf paramet ers at s ome las er tr eatments wer e observed . T he res ults rev eal ed t hat the pretreatm ent of m aize grai ns wit h H e -N e laser dos es f or 10s to 300s allevi at ed the damaging eff ect s of U V-B radiati on in the range of 7.2 KJ m −2 d −1 UVA+B radiation. KEY WORDS:He-Ne las er, Ultravi olet r adiation, prot ectiv e role, pigments, oxidative stress, antioxidants, U V-B abs orbing compounds, flav onoids. I NTRODUCTI ON :Ultraviolet radiati ons (UV) ar e a nonionizing el ectrom agnetic spectr um t hat embraces about 8-9% of s ol ar energ y (Gill et al., 2015). O zone depleti on in the strat osphere has r esult ed in an increm ent i n ultravi olet-B irradiati on (UV-B ; 280-320 nm), which has pot entiall y destr uctiv e consequences for crop yield and production and aff ect nat ural environm ents (J ulkunenTiitto et al., 2005;Siipola et al ., 2015).Enhanc ed levels of ultravi olet r adiations trigger different str uct ural, p hysiol ogical and molec ular resp ons es i n plant s (Cas ati, 2003; Kum ari et al. , 2009; Sal ama et al. , 2011;Gill et al. , 2015). A higher d ose of UV-B prov ok es impairment and modific ation of macrom olec ules like DNA , RN A, and prot eins , decreas e net phot os ynthesis and adjust the activities of sev eral antioxidant enz ym es (Gill and T ut eja, 2010;Sharma et al., 2012). Nevert heless , plants hav e d evel oped v arious protectiv e strategies to m anage and withst and, agai nst high UV-B irradiation. The act ual accum ulation of U V -absorbing materi als c an cert ainl y help in the prev ention of U V penetrati on to vul ner able layers of the leaf . T his kind of c ompounds build -up withi n the upper epidermal l ayers of the leav es of sev eral higher plants purs uin...

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“…According to current photobiology models, cell proliferation is primarily caused by a boost in ATP levels in the mitochondria, with cytochrome c oxidase as a receptor for 670-nm light, as suggested by several reports. [20][21][22][23][24][25] Moreover, intermittent laser treatment with irradiation parameters adjusted to the biostimulatory windows 12 was found to facilitate the uptake of drugs such as EGCG by HeLa cells.…”

Section: Discussionmentioning

confidence: 99%

670 nm Laser Light and EGCG Complementarily Reduce Amyloid-β Aggregates in Human Neuroblastoma Cells: Basis for Treatment of Alzheimer's Disease?

Sommer

Bieschke

Friedrich

et al. 2012

Photomedicine and Laser Surgery

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Objective: The aim of the present study is to present the results of in vitro experiments with possible relevance in the treatment of Alzheimer's disease (AD). Background Data: Despite intensive research efforts, there is no treatment for AD. One root cause of AD is the extra-and intracellular deposition of amyloid-beta (Ab) fibrils in the brain. Recently, it was shown that extracellular Ab can enter brain cells, resulting in neurotoxicity. Methods: After internalization of Ab 42 into human neuroblastoma (SH-EP) cells, they were irradiated with moderately intense 670-nm laser light (1000 Wm -2 ) and/or treated with epigallocatechin gallate (EGCG). Results: In irradiated cells, Ab 42 aggregate amounts were significantly lower than in nonirradiated cells. Likewise, in EGCGtreated cells, Ab 42 aggregate amounts were significantly lower than in non-EGCG-treated cells. Except for the cells simultaneously laden with Ab 42 and EGCG, there was a significant increase in cell numbers in response to laser irradiation. EGCG alone had no effect on cell proliferation. Laser irradiation significantly increased ATP levels in Ab 42 -free cells, when compared to nonirradiated cells. Laser-induced clearance of Ab 42 aggregates occurred at the expense of cellular ATP. Conclusions: Irradiation with moderate levels of 670-nm light and EGCG supplementation complementarily reduces Ab aggregates in SH-EP cells. Transcranial penetration of moderate levels of red to near-infrared (NIR) light has already been amply exploited in the treatment of patients with acute stroke; the blood-brain barrier (BBB) penetration of EGCG has been demonstrated in animals. We hope that our approach will inspire a practical therapy for AD.

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Helium–Neon Laser Irradiation Stimulates Cell Proliferation through Photostimulatory Effects in Mitochondria

Cited by 218 publications

References 35 publications

A Comparative Study of 660 nm Low-Level Laser and Light Emitted Diode in Proliferative Effects of Fibroblast Cells

A Comparative Study of 660 nm Low-Level Laser and Light Emitted Diode in Proliferative Effects of Fibroblast Cells

Role of He-Ne laser pre-treatment in protecting Zea mays against the deleterious effects of ultraviolet radiations

670 nm Laser Light and EGCG Complementarily Reduce Amyloid-β Aggregates in Human Neuroblastoma Cells: Basis for Treatment of Alzheimer's Disease?

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