Helium Ion Microscopy of Uncoated Pine Leaves

Kim, Ki-Woo

doi:10.9729/am.2012.42.3.147

Cited by 5 publications

(3 citation statements)

References 10 publications

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“…Salt spray has been shown to cause microtopographical symptoms, such as coalesced epicuticular waxes and flaccid subsidiary cells, in Pinus parviflora needles (Krause, 1982). Several types of epicuticular waxes, including coiled rodlets and tubules, have been reported in the needles of allied Pinus species, including P. koraiensis (Kim, Lee, Bae, & Kim, 2011; Kim, 2012) and P. densiflora (Kim, Lee, Kim, et al., 2011). Based on the electron micrographs of P. thunbergii needles (Ghimire et al., 2014), no distinct epicuticular waxes have been discerned in the stomata on the asymptomatic needles.…”

Section: Discussionmentioning

confidence: 99%

Electron microscopy and X‐ray microanalysis of Pinus thunbergii needles: Stomatal penetration by seawater and solutes

Park,

Seo,

Kim

2023

Forest Pathology

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Seawater salt deposition was investigated in the needles of Pinus thunbergii trees growing in the coastal area of Pohang, Korea. Both green asymptomatic and brown necrotized needles were air‐dried and processed for field‐emission scanning electron microscopy and X‐ray microanalysis. Seawater salt crystals appeared randomly deposited on the asymptomatic needles. No distinct epicuticular waxes were found in the stomata or on the needle surface. The salt crystals, which varied in size, were observed in the stomatal chambers as well as on the apertures of the Florin rings, and similar crystals were also observed on the necrotized brown needles. Sodium (Na) and chloride (Cl) were prevalent on the needles and mostly co‐localized in the crystals. Magnesium was partially co‐localized with Cl, but often co‐occurred with potassium. Depending on the elemental composition of salts, two types of salt deposition were observed on the needles: (i) the coffee‐ring pattern and (ii) the uniform pattern. The apparent NaCl crystal depositions in the stomatal chambers indicated the stomatal penetration by seawater and solutes in the seawater‐sprayed needles of P. thunbergii. These results provide insights into the initial phase of seawater salt‐induced injury occurring through the stomatal pathway in pine species distributed in coastal areas.

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Section: Discussionmentioning

confidence: 99%

Electron microscopy and X‐ray microanalysis of Pinus thunbergii needles: Stomatal penetration by seawater and solutes

Park,

Seo,

Kim

2023

Forest Pathology

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“…HIM imaging of small animals and plants has been around since the early years of helium ion microscopy, when pollen samples [7,8] and pine leaves [14] were imaged. The first in-depth and notable HIM imaging demonstration of small animals was done in 2012 by Boden et al [12], when the intricate micro-and nanostructures responsible for the structural colouration of the wings of two different butterfly species, Papilio ulysses (Blue Mountain Butterfly) and Parides sesostris (Emerald-patched Cattleheart), were imaged to a level of detail not obtained previously with SEM.…”

Section: Imaging Animals and Plantsmentioning

confidence: 99%

Bio-imaging with the helium-ion microscope: A review

Schmidt¹,

Byrne²,

Maasilta³

2021

Beilstein J. Nanotechnol.

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Scanning helium-ion microscopy (HIM) is an imaging technique with sub-nanometre resolution and is a powerful tool to resolve some of the tiniest structures in biology. In many aspects, the HIM resembles a field-emission scanning electron microscope (FE-SEM), but the use of helium ions rather than electrons provides several advantages, including higher surface sensitivity, larger depth of field, and a straightforward charge-compensating electron flood gun, which enables imaging of non-conductive samples, rendering HIM a promising high-resolution imaging technique for biological samples. Starting with studies focused on medical research, the last decade has seen some particularly spectacular high-resolution images in studies focused on plants, microbiology, virology, and geomicrobiology. However, HIM is not just an imaging technique. The ability to use the instrument for milling biological objects as small as viruses offers unique opportunities which are not possible with more conventional focused ion beams, such as gallium. Several pioneering technical developments, such as methods to couple secondary ion mass spectrometry (SIMS) or ionoluminescence with the HIM, also offer the possibility for new and exciting research on biological materials. In this review, we present a comprehensive overview of almost all currently published literature which has demonstrated the application of HIM for imaging of biological specimens. We also discuss some technical features of this unique type of instrument and highlight some of the new advances which will likely become more widely used in the years to come.

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“…0.25 nm), (2) greater surface sensitivity, (3) greater contrast, (4) larger depth-of-field, (5) efficient charge control, (6) reduced specimen damage, and (7) nanomachining capability (Bell, 2009;Vanden Berg-Foels et al, 2012). There is growing evidence that the HIM has substantial potential for high-resolution imaging of uncoated insulating biological specimens at the nanoscale (Bazou et al, 2011;Alkemade et al, 2012;Boden et al, 2012;Kim, 2012).…”

Section: Introductionmentioning

confidence: 99%

Biological Applications of Helium Ion Microscopy

Kim¹

2013

Applied Microscopy

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Helium Ion Microscopy of Uncoated Pine Leaves

Cited by 5 publications

References 10 publications

Electron microscopy and X‐ray microanalysis of Pinus thunbergii needles: Stomatal penetration by seawater and solutes

Electron microscopy and X‐ray microanalysis of Pinus thunbergii needles: Stomatal penetration by seawater and solutes

Bio-imaging with the helium-ion microscope: A review

Biological Applications of Helium Ion Microscopy

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