Helium Ion Microscopy for Imaging and Quantifying Porosity at the Nanoscale

Burch, Matthew J.; Ievlev, Anton V.; Mahady, Kyle; Hysmith, Holland; Rack, Philip D.; Belianinov, Alex; Ovchinnikova, Olga S.

doi:10.1021/acs.analchem.7b04418

Cited by 17 publications

(10 citation statements)

References 43 publications

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“…[ 1 ] These properties make the technique a useful tool for nanoscale research. HIM has been used for imaging nanoporous SiO 2 , [ 2 ] cellulose nanofibrils, [ 3 ] mammalian cells, [ 4 ] and other biological samples. [ 5 ] To the best of our knowledge, we report on the first time that HIM has been used to image starch.…”

Section: Figurementioning

confidence: 99%

Helium Ion Microscopy of Corn Starch

Wulff

Aucoin

2020

Starch Stärke

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Normal corn starch granules are imaged using a helium ion microscope (HIM). This relatively new imaging technique produces high‐resolution images of both the interior and exterior of the granules that allow features to be observed which had previously not been seen with scanning electron microscope. Of particular interest is the mesh‐like structure which is observed in the interior when starch granules are sectioned with a metal blade, a structure not currently part of existing models of starch architecture. HIM imaging provides a new way to characterize starch in the ongoing quest to elucidate its internal structure.

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Section: Figurementioning

confidence: 99%

Helium Ion Microscopy of Corn Starch

Wulff

Aucoin

2020

Starch Stärke

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“…Detailed surface analysis on the wear debris was performed next. Due to the powerful focusing capability of He ion beams with the probe size below nm [ 50 ], the technique enabled high-resolution imaging to identify the morphology and topography of the wear debris down to the nm scale ( Figure 4 B), as was recently shown on nanoporous materials [ 51 ]. A broad distribution of the debris size, shape and morphology was observed on a wider field of view (on the right), whereas distinct surface nanomorphologies were only revealed at the more close-up image (on the left).…”

Section: Resultsmentioning

confidence: 99%

Revealing Inflammatory Indications Induced by Titanium Alloy Wear Debris in Periprosthetic Tissue by Label-Free Correlative High-Resolution Ion, Electron and Optical Microspectroscopy

et al. 2021

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The metallic-associated adverse local tissue reactions (ALTR) and events accompanying worn-broken implant materials are still poorly understood on the subcellular and molecular level. Current immunohistochemical techniques lack spatial resolution and chemical sensitivity to investigate causal relations between material and biological response on submicron and even nanoscale. In our study, new insights of titanium alloy debris-tissue interaction were revealed by the implementation of label-free high-resolution correlative microscopy approaches. We have successfully characterized its chemical and biological impact on the periprosthetic tissue obtained at revision surgery of a fractured titanium-alloy modular neck of a patient with hip osteoarthritis. We applied a combination of photon, electron and ion beam micro-spectroscopy techniques, including hybrid optical fluorescence and reflectance micro-spectroscopy, scanning electron microscopy (SEM), Energy-dispersive X-ray Spectroscopy (EDS), helium ion microscopy (HIM) and micro-particle-induced X-ray emission (micro-PIXE). Micron-sized wear debris were found as the main cause of the tissue oxidative stress exhibited through lipopigments accumulation in the nearby lysosome. This may explain the indications of chronic inflammation from prior histologic examination. Furthermore, insights on extensive fretting and corrosion of the debris on nm scale and a quantitative measure of significant Al and V release into the tissue together with hydroxyapatite-like layer formation particularly bound to the regions with the highest Al content were revealed. The functional and structural information obtained at molecular and subcellular level contributes to a better understanding of the macroscopic inflammatory processes observed in the tissue level. The established label-free correlative microscopy approach can efficiently be adopted to study any other clinical cases related to ALTR.

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“…Image analysis consisted of three main aspects: image pre-processing, segmentation, feature extraction, and quantification [60][61][62]. We used open-source Python 2.7 for all analysis steps.…”

Section: Discussionmentioning

confidence: 99%

Direct Write of 3D Nanoscale Mesh Objects with Platinum Precursor via Focused Helium Ion Beam Induced Deposition

et al. 2020

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The next generation optical, electronic, biological, and sensing devices as well as platforms will inevitably extend their architecture into the 3rd dimension to enhance functionality. In focused ion beam induced deposition (FIBID), a helium gas field ion source can be used with an organometallic precursor gas to fabricate nanoscale structures in 3D with high-precision and smaller critical dimensions than focused electron beam induced deposition (FEBID), traditional liquid metal source FIBID, or other additive manufacturing technology. In this work, we report the effect of beam current, dwell time, and pixel pitch on the resultant segment and angle growth for nanoscale 3D mesh objects. We note subtle beam heating effects, which impact the segment angle and the feature size. Additionally, we investigate the competition of material deposition and sputtering during the 3D FIBID process, with helium ion microscopy experiments and Monte Carlo simulations. Our results show complex 3D mesh structures measuring ~300 nm in the largest dimension, with individual features as small as 16 nm at full width half maximum (FWHM). These assemblies can be completed in minutes, with the underlying fabrication technology compatible with existing lithographic techniques, suggesting a higher-throughput pathway to integrating FIBID with established nanofabrication techniques.

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Helium Ion Microscopy for Imaging and Quantifying Porosity at the Nanoscale

Cited by 17 publications

References 43 publications

Helium Ion Microscopy of Corn Starch

Helium Ion Microscopy of Corn Starch

Revealing Inflammatory Indications Induced by Titanium Alloy Wear Debris in Periprosthetic Tissue by Label-Free Correlative High-Resolution Ion, Electron and Optical Microspectroscopy

Direct Write of 3D Nanoscale Mesh Objects with Platinum Precursor via Focused Helium Ion Beam Induced Deposition

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