Heliox-Driven Nebulization Has a Positive Effect on the Lung Function in Lipopolysaccharide-Induced Chronic Obstructive Pulmonary Disease Rat Model

Wu, Wenwen; Chen, Xi; Liu, Xiaohan; Liu, Chengyuan; Lu, Gendi

doi:10.12659/msm.896736

Cited by 5 publications

(5 citation statements)

References 30 publications

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“…In this study, we found that long-term exposure of PM2.5 resulted in a significant decrease in pulmonary compliance and forced pulmonary ventilation in mice. This result is consistent with previous studies reporting that the dynamic lung compliance is decreased in COPD [ 34 , 35 ]. In accordance with these observations, we found that PM2.5 exposure induced mouse pulmonary emphysema and reduced the mean number of alveoli in the mouse lung.…”

Section: Discussionsupporting

confidence: 94%

Impaired AT2 to AT1 cell transition in PM2.5-induced mouse model of chronic obstructive pulmonary disease

Lin

Zhong

et al. 2022

Respir Res

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Background Particular matter 2.5 (PM2.5) is one of the most important air pollutant, and it is positively associated with the development of chronic obstructive pulmonary disease (COPD). However, the precise underlying mechanisms through which PM2.5 promotes the development of COPD remains largely unknown. Methods Mouse alveolar destruction were determined by histological analysis of lung tissues and lung function test. Alveolar type II cells (AT2) to alveolar type I cells (AT1) transition in PM2.5-induced COPD mouse model was confirmed via immunofluorescence staining and qPCR analysis. The differentially expressed genes in PM2.5-induced COPD mouse model were identified by RNA-sequencing of alveolar epithelial organoids and generated by bioinformatics analysis. Results In this study, we found that 6 months exposure of PM2.5 induced a significantly decreased pulmonary compliance and resulted in pulmonary emphysema in mice. We showed that PM2.5 exposure significantly reduced the AT2 to AT1 cell transition in vitro and in vivo. In addition, we found a reduced expression of the intermediate AT2-AT1 cell process marker claudin 4 (CLDN4) at day 4 of differentiation in mouse alveolar organoids treated with PM2.5, suggesting that PM2.5 exposure inhibited AT2 cells from entering the transdifferentiation process. RNA-sequencing of mouse alveolar organoids showed that several key signaling pathways that involved in the AT2 to AT1 cell transition were significantly altered including the Wnt signaling, MAPK signaling and signaling pathways regulating pluripotency of stem cells following PM2.5 exposure. Conclusions In summary, these data demonstrate a critical role of AT2 to AT1 cell transition in PM2.5-induced COPD mouse model and reveal the signaling pathways that potentially regulate AT2 to AT1 cell transition during this process. Our findings therefore advance the current knowledge of PM2.5-induced COPD and may lead to a novel therapeutic strategy to treat this disease.

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Section: Discussionsupporting

confidence: 94%

Impaired AT2 to AT1 cell transition in PM2.5-induced mouse model of chronic obstructive pulmonary disease

Lin

Zhong

et al. 2022

Respir Res

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“…Research shows that helium can reduce the inflammatory reaction in lung damage and has anti‐inflammatory effects. Animal experiments have shown that helium can decrease alveolar hemorrhage in mice, neutrophil invasion in lung tissue, edema, and pulmonary hyaline membrane occurrence 38,39 . Many studies suggest that meconium is a potent activator of inflammatory mediators, including cytokines, complement, prostaglandins and reactive oxygen species.…”

Section: Discussionmentioning

confidence: 99%

A randomized single‐center controlled trial of synchronized intermittent mandatory ventilation with heliox in newborn infants with meconium aspiration syndrome

Tang²,

Shen

et al. 2021

Pediatric Pulmonology

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Objective This study aimed to investigate the beneficial effects of synchronized intermittent mandatory ventilation (SIMV) with heliox in newborn infants with meconium aspiration syndrome (MAS). Methods Seventy‐one newborn infants with MAS in the neonatal intensive care unit (NICU) of Daping Hospital of Army Medical University were enrolled in the trial. Infants treated with SIMV were randomized and divided into the heliox group (n = 35) and control group (n = 36). The heliox group received heliox for 6 h followed by air‐oxygen mixed gas, and the control group received air‐oxygen mixed gas. The primary outcome measures were PaO2/FiO2 (P/F) and the extubation time. The secondary outcome measures were the incidence of mechanical ventilation complications, hospital length of stay in the NICU, blood gas analysis, and inflammation markers. Results The P/F in the heliox group was significantly better than that in the control group (p < .001). The extubation time and hospital length of stay in the NICU in the heliox group were shorter than those in the control group (p < .001). The inflammation markers at 6 h and myocardial injury markers at 24 h were decreased compared with those at 0 h, and those in the heliox group were more significantly decreased than those in the control group ([interleukin {IL}‐6/IL‐8/tumor necrosis factor α] p < .001, [C‐reaction protein] p = .012; [creatine kinase] p < .001, [CK‐MB] p = .041). Conclusion Heliox appears to be more effective in reducing the length of ventilation and increasing carbon dioxide eliminations than an air‐oxygen mixture in infants with MAS under the support of SIMV.

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“…With low density and high diffusion, heliox can reduce the occurrence of turbulence in the airway and effectively reduce the airway resistance, subsequently reducing endogenous PEEP and lung volume. In this way, venous return and pulmonary circulation resistance can be relatively stable, and hemodynamic status can be improved to a certain extent [24][25][26]. Therefore in this study, compared with nitrogen-oxygen ventilation at a relatively high PEEP level, heliox ventilation reduced airway pressure and CVP and increased ScvO 2 in older hypertensive patients, without affecting heart rate or causing signi cant uctuations in blood pressure.…”

Section: Discussionmentioning

confidence: 92%

“…Heliox is di cult to prepare, expensive, and easy to escape from tightly sealed containers due to its high diffusion, resulting in high therapeutic cost and limited clinical application [25]. In this study, the hermetic breathing circuit was innovatively applied for heliox ventilation to recycle helium gas.…”

Section: Discussionmentioning

confidence: 99%

Heliox ventilation in older hypertensive ICU patients improves hemodynamics: A randomized controlled study

Zhou,

Chen,

Zhuang

et al. 2024

Preprint

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Background: Conventional mechanical ventilation has adverse impacts on hemodynamics of older hypertensive ICU patients. Limited studies have addressed way to ameliorate these negative effects. This study aimed at determining if heliox ventilation mitigated the side-effects of conventional mechanical ventilation in senile hypertensive patients. Methods: Seventy-nine older adults aged 65 to 95 years with essential hypertension who underwent invasive mechanical ventilation treatment, were divided into two groups: a control group of nitrogen-oxygen ventilation (n = 40) and an experimental group of heliox ventilation (n = 39). The control group received conventional room air ventilation and the experimental group innovatively adopted the closed heliox ventilation technique. All patients had appropriate positive end-expiratory pressure (PEEP) values (5-8cmH2O) using the optimal oxygenation method under VCV mode throughout the study. Blood pressure, central venous pressure (CVP), central venous oxygen saturation (ScvO2), heart rate (HR), and airway pressure were measured every hour. Results: An increase of CVP and airway pressure coupled with a decrease of blood pressure and ScvO2 were observed in the control group as expected (p < 0.05). Comparatively, CVP and airway pressure reductions with an increased ScvO2 were observed in the experimental group (p < 0.05). In the experimental group, blood pressure and HR did not change significantly. Conclusions: Heliox ventilation improves cardiac function and blood pressure in older hypertensives as compared with nitrogen-oxygen ventilation. Trial registration This trial was registered. The Chinese trial registration number is ChiCTR2100043945. The date of registration is 6-3-2021. The registered name is that heliox ventilation improves hemodynamics in older patients with hypertention admitted to the intensive care unit.

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Heliox-Driven Nebulization Has a Positive Effect on the Lung Function in Lipopolysaccharide-Induced Chronic Obstructive Pulmonary Disease Rat Model

Cited by 5 publications

References 30 publications

Impaired AT2 to AT1 cell transition in PM2.5-induced mouse model of chronic obstructive pulmonary disease

Impaired AT2 to AT1 cell transition in PM2.5-induced mouse model of chronic obstructive pulmonary disease

A randomized single‐center controlled trial of synchronized intermittent mandatory ventilation with heliox in newborn infants with meconium aspiration syndrome

Heliox ventilation in older hypertensive ICU patients improves hemodynamics: A randomized controlled study

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