Heligmosomoides polygyrus: EAE remission is correlated with different systemic cytokine profiles provoked by L4 and adult nematodes

Donskow‐Łysoniewska, Katarzyna; Krawczak, Katarzyna; Doligalska, Maria

doi:10.1016/j.exppara.2012.07.009

Cited by 27 publications

(27 citation statements)

References 18 publications

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“…Indeed, TGF-b is also induced by H. polygyrus infection. Although primarily found at the site of infection, increased levels of serum TGF-b have also been reported in H. polygyrus-infected mice (41). We found that splenic CD4 cells from mice with chronic H. polygyrus infection express more TGFb latency-associated peptide (LAP) compared with naive mice (S2 D-F).…”

Section: Discussionmentioning

confidence: 49%

Chronic Gastrointestinal Nematode Infection Mutes Immune Responses to Mycobacterial Infection Distal to the Gut

Obieglo

Feng

Bollampalli

et al. 2016

The Journal of Immunology

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Helminth infections have been suggested to impair the development and outcome of Th1 responses to vaccines and intracellular microorganisms. However, there are limited data regarding the ability of intestinal nematodes to modulate Th1 responses at sites distal to the gut. In this study, we have investigated the effect of the intestinal nematode Heligmosomoides polygyrus bakeri on Th1 responses to Mycobacterium bovis bacillus Calmette–Guérin (BCG). We found that H. polygyrus infection localized to the gut can mute BCG-specific CD4+ T cell priming in both the spleen and skin-draining lymph nodes. Furthermore, H. polygyrus infection reduced the magnitude of delayed-type hypersensitivity (DTH) to PPD in the skin. Consequently, H. polygyrus–infected mice challenged with BCG had a higher mycobacterial load in the liver compared with worm-free mice. The excretory–secretory product from H. polygyrus (HES) was found to dampen IFN-γ production by mycobacteria-specific CD4+ T cells. This inhibition was dependent on the TGF-βR signaling activity of HES, suggesting that TGF-β signaling plays a role in the impaired Th1 responses observed coinfection with worms. Similar to results with mycobacteria, H. polygyrus–infected mice displayed an increase in skin parasite load upon secondary infection with Leishmania major as well as a reduction in DTH responses to Leishmania Ag. We show that a nematode confined to the gut can mute T cell responses to mycobacteria and impair control of secondary infections distal to the gut. The ability of intestinal helminths to reduce DTH responses may have clinical implications for the use of skin test–based diagnosis of microbial infections.

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Section: Discussionmentioning

confidence: 49%

Chronic Gastrointestinal Nematode Infection Mutes Immune Responses to Mycobacterial Infection Distal to the Gut

Obieglo

Feng

Bollampalli

et al. 2016

The Journal of Immunology

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“…In models of autoimmunity, H. polygyrus infection has been shown to be suppressive in the experimental autoimmune encephalomyelitis (EAE) model of multiple sclerosis 9 , and suppression can be transferred with either CD4+ T cells or CD19+ B cells from infected mice 24 .…”

Section: Discussionmentioning

confidence: 99%

“…Active infection with H. polygyrus has been shown to protect against immunopathology in many animal models of autoimmunity [7][8][9][10] , colitis 11,12 and allergy [13][14][15][16] . There has consequently been great interest in the potential of Excretory-Secretory molecules from this parasite ("HES") to down-modulate pathology in vivo 17,18 .…”

mentioning

confidence: 99%

Cultivation of <em>Heligmosomoides Polygyrus:</em> An Immunomodulatory Nematode Parasite and its Secreted Products

Johnston

Robertson

Harcus

et al. 2015

JoVE

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Heligmosomoides polygyrus (formerly known as Nematospiroides dubius, and also referred to by some as H. bakeri) is a gastrointestinal helminth that employs multiple immunomodulatory mechanisms to establish chronic infection in mice and closely resembles prevalent human helminth infections. H. polygyrus has been studied extensively in the field of helminth-derived immune regulation and has been found to potently suppress experimental models of allergy and autoimmunity (both with active infection and isolated secreted products). The protocol described in this paper outlines management of the H. polygyrus life cycle for consistent production of L3 larvae, recovery of adult parasites, and collection of their excretory-secretory products (HES).

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“…EAE mimics several of the key clinical and pathological features of MS, and is a proven tool of testing therapies effective in human studies T Hart et al 2011;Farooqi et al 2010). However, EAE best models the effects of an intervention for an individual inflammatory attack and provides little insight on the long-term impact that an intervention could have on the course of a recurring conditions such as MS. To date, 13 EAE studies (Sewell et al 2003;Zheng et al 2008;La Flamme et al 2003;Kuijk et al 2012;Donskow-Lysoniewska et al 2012;Sofronic-Milosavljevic et al 2013;Zhu et al 2012;Wu et al 2010;Walsh et al 2009;Wilson et al 2010;Reyes et al 2011;Gruden-Movsesijan et al 2010;Chiuso-Minicucci et al 2011) analysing helminth treatment effects on EAE have been reported (Table 1). Parasite effects on EAE have been recently reviewed and compared to EAE studies involving protozoan organisms (Hasseldam et al 2013;Fleming 2013).…”

Section: Lessons From Studies Of Helminth Treatment In Experimental Mmentioning

confidence: 99%

“…Protozoan infections are systemic and effective at ameliorating EAE symptoms during all phases (including late acute, clinically overt phases) (Hasseldam et al 2013). In all but two of the EAE studies in which the treatment induced changes (Donskow-Lysoniewska et al 2012;Sofronic-Milosavljevic et al 2013), the more localized helminthic activity was mostly effective in the pre-immunisation and induction phases of EAE. For example, treatment with soluble egg antigen from Schistosoma spp.…”

Section: Lessons From Studies Of Helminth Treatment In Experimental Mmentioning

confidence: 99%

Emerging and Evolving Topics in Multiple Sclerosis Pathogenesis and Treatments

Flamme¹,

Orian²

2015

Current Topics in Behavioral Neurosciences

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Current Topics in Behavioral Neurosciences provides critical and comprehensive discussions of the most significant areas of behavioral neuroscience research, written by leading international authorities. Each volume offers an informative and contemporary account of its subject, making it an unrivalled reference source. Titles in this series are available in both print and electronic formats.With the development of new methodologies for brain imaging, genetic and genomic analyses, molecular engineering of mutant animals, novel routes for drug delivery, and sophisticated cross-species behavioral assessments, it is now possible to study behavior relevant to psychiatric and neurological diseases and disorders on the physiological level. The Behavioral Neurosciences series focuses on ''translational medicine'' and cutting-edge technologies. Preclinical and clinical trials for the development of new diagnostics and therapeutics as well as prevention efforts are covered whenever possible.

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Heligmosomoides polygyrus: EAE remission is correlated with different systemic cytokine profiles provoked by L4 and adult nematodes

Cited by 27 publications

References 18 publications

Chronic Gastrointestinal Nematode Infection Mutes Immune Responses to Mycobacterial Infection Distal to the Gut

Chronic Gastrointestinal Nematode Infection Mutes Immune Responses to Mycobacterial Infection Distal to the Gut

Cultivation of <em>Heligmosomoides Polygyrus:</em> An Immunomodulatory Nematode Parasite and its Secreted Products

Emerging and Evolving Topics in Multiple Sclerosis Pathogenesis and Treatments

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