Helicobacter pylori Western cagA genotype in Egyptian patients with upper gastrointestinal disease

Diab, Manal; Shemis, Mohamed; Gamal, Doaa; El‐Shenawy, A. I.; El-Ghannam, Maged; El-Sherbini, Effat; Saber, Mohamed

doi:10.1016/j.ejmhg.2018.06.003

Cited by 12 publications

(10 citation statements)

References 25 publications

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“…Our results agree with those reported by Ezzat et al [17], they detected cagA gene in 13.3% of gastritis patients. Similarly, El Shenawy et al [13] and Diab et al [18] found the gene in 18.2% and 18.5% of H. pylori strains isolated from gastritis cases, respectively. Other studies reported prevalence about 33-38% [10,12,19].…”

Section: Discussionmentioning

confidence: 83%

Detection of Helicobacter pylori oipA and dupA genes among dyspeptic patients with chronic gastritis

Elsayed

Musa

Eltabbakh

et al. 2020

Alexandria Journal of Medicine

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Helicobacter pylori (H. pylori): is a microbe with wide genetic diversity that infects the stomach of most people in developing countries, leading to several clinical outcomes among different individuals such as gastritis, ulcers, or gastric cancer. Outer inflammatory protein A (oipA) and duodenal ulcer promoting (dupA) genes are among the possible virulence factors which determine the patient outcome. Aim: To detect oipA and dupA genes of H. pylori among dyspeptic Egyptian patients, and to investigate their correlation with the varying degrees of the associated chronic gastritis. Methods: The study enrolled 50 patients with dyspepsia, attending the Gastrointestinal Endoscopy unit of the Gastroenterology and Tropical Departments at Ain Shams University Hospital for upper gastrointestinal endoscopy, in the period between, June and, December 2019. Four antral gastric biopsies were taken from each patient for polymerase chain reaction assay to detect the virulence genes oipA, dupA, and cagA and for histopathological assessment. Results: Forty patients were H. pylori positive by histopathology and PCR. cagA, oipA, and dupA were identified in 6 (15%), 13 (32.5%), 9 (22.5%) of biopsies, respectively. Both cagA and oipA genes were highly significantly associated with increasing the severity of gastritis. Only oipA virulence gene showed a highly significant association with gastroduodenitis. There was a highly significant moderate association between cagA and oipA genes. Conclusion: oipA could be a virulence biomarker that serves a great value in predicting the progress of gastric mucosal damage in patients with chronic gastritis, and targeting antimicrobial therapy in those patients to prevent severe gastroduodenal diseases.

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Section: Discussionmentioning

confidence: 83%

Detection of Helicobacter pylori oipA and dupA genes among dyspeptic patients with chronic gastritis

Elsayed

Musa

Eltabbakh

et al. 2020

Alexandria Journal of Medicine

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“…The cagA gene can cause considerable inflammation and severe infection in the gastrointestinal system and has been associated with conditions such as peptic ulcer and gastric cancer. 6,7 Several diagnostic methods are used to identify H. pylori infections including: (i) invasive methods (e.g., endoscopy and culture) followed by histopathology and/ or various molecular and nucleic acid amplification tests; and (ii) non-invasive endoscopy-independent methods such as urea breath tests, fecal antigen tests, and serological tests. 8 Among non-invasive methods, serological tests are simple, rapid, inexpensive, and convenient.…”

Section: Introductionmentioning

confidence: 99%

“…The cagA gene can cause considerable inflammation and severe infection in the gastrointestinal system and has been associated with conditions such as peptic ulcer and gastric cancer. 6,7…”

Section: Introductionmentioning

confidence: 99%

Associations between seroprevalence of Helicobacter pylori and ABO/rhesus blood group antigens in healthy blood donors in southwest Iran

et al. 2021

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Objective To investigate correlations between ABO/rhesus (Rh) blood group antigens and anti- Helicobacter pylori and anti-cytotoxin-associated gene A (CagA) seropositivity in blood donors. Methods A total of 311 blood donors were enrolled. ABO and Rh blood groups were determined using hemagglutination tests. Specific anti- H. pylori IgG and anti-CagA IgG antibodies in sera were quantitated by enzyme-linked immunosorbent assay. Correlations between blood groups and anti- H. pylori and anti-CagA seropositivity were evaluated using the Chi-square test. Results O+ was the most frequent blood type (38%, n = 118). Anti- H. pylori IgG seropositivity was observed in 240 (77.2%) blood donors, while anti-CagA IgG seropositivity was observed in 132 (42.5%) blood donors. Although seropositivity rates for both anti- H. pylori and anti-CagA IgG were higher in individuals with blood type O, no statistically significant associations were observed between seropositivity and any ABO/Rh blood groups. Conclusion Individuals with blood type O may have higher rates of H. pylori seropositivity.

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“…Так, в исследовании Y. Yamaoka et al 2011 В 2018 г. группой ученых под руководством Manal Diab было опубликовано, что отсутствие более вирулентного восточно-азиатского генотипа СagA, который является самым сильным фактором риска канцерогенеза желудка, может объяснить очень низкий уровень рака желудка среди населения Египта по сравнению с другими частями мира. Однако они подчеркнули, что этот вывод требует дальнейших молекулярных исследований с использованием секвенирования всего генома и большего количества образцов для определения точного нехарактерного генотипа СagA для выявления фактического риска развития гастродуоденальных заболеваний в Египте [15]. Также исследователи отметили, что их показатель сопоставим с предыдущими исследованиями из Иордании и Ливана [12,14].…”

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Risk assessment of pathological conditions caused by circulating H. pylori strains

Karimova¹,

Sobirova²,

Karimov³

2020

Bul. of the Club of Pancr.

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In recent years, there has been an increase in the prevalence of inflammatory and destructive diseases of the gastroduodenal zone, which is primarily explained by Helicobacter pylori (H. pylori) infection. One of the main factors of H. pylori pathogenicity is presence of cytotoxin-associated gene — CagA. It is known that CagA-positive H. pylori strains are associated with the development of atrophy, tumor invasion and rapid metastasis. A number of recently published studies have revealed that CagA is a polymorphic gene which contains a different number of repetitive sequences located in the 3’ region. Each repetitive region of CagA contains Glu-Pro-Ile-Tyr-Ala (EPIYA) profiles including tyrosine phosphorylation. Depending on the sequence of the EPIYA profile, there are 4 segments: EPIYA-A, EPIYA-B, EPIYA-C, EPIYA-D, each containing a repetitive region. Geographical features of the prevalence of H. pylori strains depending on the sequence of EPIYA have been revealed: EPIYA-A region of the western isolates of this bacterium is associated with EPIYA-A, EPIYA-B, EPIYA-C segments, while the eastern CagA-positive H. pylori isolates are characterized by the A-B-D type of the CagA gene. Data illustrating the strong correlation between the western CagA-positive H. pylori strains, which have a repeating EPIYA-C segment, and the development of precancerous states, as well as gastric cancer, are presented. H. pylori strains containing simultaneously A-B motives of EPIYA or one C-type of the CagA gene are associated with a 7-fold increase of risk of gastric cancer compared to CagA-negative strains; presence of two or more EPIYA-C motives is associated with a 30-fold increase of this risk.

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Helicobacter pylori Western cagA genotype in Egyptian patients with upper gastrointestinal disease

Cited by 12 publications

References 25 publications

Detection of Helicobacter pylori oipA and dupA genes among dyspeptic patients with chronic gastritis

Detection of Helicobacter pylori oipA and dupA genes among dyspeptic patients with chronic gastritis

Associations between seroprevalence of Helicobacter pylori and ABO/rhesus blood group antigens in healthy blood donors in southwest Iran

Risk assessment of pathological conditions caused by circulating H. pylori strains

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