Helicobacter pylori vacA, cagA and iceA genotypes in dyspeptic patients from southwestern region, Saudi Arabia: distribution and association with clinical outcomes and histopathological changes

Akeel, Mohammed; Shehata, Atef; Elhafey, Ahmed; Elmakki, Erwa; Aboshouk, Thanaa; Ageely, Hussein; Mahfouz, Mohammed Salih

doi:10.1186/s12876-019-0934-z

Cited by 37 publications

(53 citation statements)

References 44 publications

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“…Cytotoxin Associated Gene A and E (cagA and cagE), Induced by Contact with the Epithelium Antigen (iceA), Outer Inflammatory Protein Antigen (oipA), and Blood Group Antigen-Binding Adhesin gene (babA). 8,9 The vacA gene is polymorphic, containing mutable signals (type s1 or s2) and mid-regions (type m1 or m2). The s1 type is further alienated into s1a, s1b and s1c and the m1 into m1a and m1b alleles.…”

Section: Introductionmentioning

confidence: 99%

“…The cagA gene is an indicator for the genomic pathogenicity island of c. 40 kb [cag pathogenicity island (cag-PAI)] and its activity is believed to be cooperated with interleukin 8 secretion, local inflammation, and severe and/or complicated occurrence of peptic ulcers and gastrointestinal disorders. 8,9 CagE gene was found to serve as an improved biomarker of an intact cag-PAI in patients with severe gastrointestinal disorders. 8,9 BabA2 gene mediates adherence of H. pylori to human Lewis b blood-group antigens on gastric epithelial cells.…”

Section: Introductionmentioning

confidence: 99%

“…8,9 CagE gene was found to serve as an improved biomarker of an intact cag-PAI in patients with severe gastrointestinal disorders. 8,9 BabA2 gene mediates adherence of H. pylori to human Lewis b blood-group antigens on gastric epithelial cells. 8,9 OipA is a significant virulence marker which is associated with clinically imperative presentation of peptic ulcers, such as enhanced interleukin-8 secretion and increased inflammation.…”

Section: Introductionmentioning

confidence: 99%

“…8,9 BabA2 gene mediates adherence of H. pylori to human Lewis b blood-group antigens on gastric epithelial cells. 8,9 OipA is a significant virulence marker which is associated with clinically imperative presentation of peptic ulcers, such as enhanced interleukin-8 secretion and increased inflammation. 8,9 The iceA gene was detected in the H. pylori recovered from patients with gastrointestinal disorders.…”

Section: Introductionmentioning

confidence: 99%

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Phenotypic and Genotypic Assessment of Antibiotic Resistance and Genotyping of vacA, cagA, iceA, oipA, cagE, and babA2 Alleles of Helicobacter pylori Bacteria Isolated from Raw Meat

Mashak¹,

Jafariaskari²,

Alavi³

et al. 2020

IDR

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Background: Foodstuffs with animal origins, particularly meat, are likely reservoirs of Helicobacter pylori. Purpose: An existing survey was accompanied to assess phenotypic and genotypic profiles of antibiotic resistance and genotyping of vacA, cagA, cagE, iceA, oipA, and babA2 alleles amongst the H. pylori bacteria recovered from raw meat. Methods: Six-hundred raw meat samples were collected and cultured. H. pylori isolates were tested using disk diffusion and PCR identification of antibiotic resistance genes and genotyping. Results: Fifty-two out of 600 (8.66%) raw meat samples were contaminated with H. pylori. Raw ovine meat (13.07%) had the uppermost contamination. H. pylori bacteria displayed the uppermost incidence of resistance toward tetracycline (82.69%), erythromycin (80.76%), trimethoprim (65.38%), levofloxacin (63.46%), and amoxicillin (63.46%). All H. pylori bacteria had at least resistance toward one antibiotic, even though incidence of resistance toward more than eight antibiotics was 28.84%. Total distribution of rdxA, pbp1A, gyrA, and cla antibiotic resistance genes were 59.61%, 51.92%, 69.23%, and 65.38%, respectively. VacA s1a (84.61%), s2 (76.92%), m1a (50%), m2 (39.13%), iceA1 (38.46%), and cagA (55.76%) were the most generally perceived alleles. S1am1a (63.46%), s2m1a (53.84%), s1am2 (51.92%), and s2m2 (42.30%) were the most generally perceived genotyping patterns. Frequency of cagA-, oipA-, and babA2-genotypes were 44.23%, 73.07%, and 80.76%, respectively. A total of 196 combined genotyping patterns were also perceived. Conclusion: The role of raw meat, particularly ovine meat, in transmission of virulent and resistant H. pylori bacteria was determined. VacA and cagA genotypes had the higher incidence. CagE-, babA2-, and oipA-H. pylori bacteria had the higher distribution. Supplementary surveys are compulsory to originate momentous relations between distribution of genotypes, antibiotic resistance, and antibiotic resistance genes.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phenotypic and Genotypic Assessment of Antibiotic Resistance and Genotyping of vacA, cagA, iceA, oipA, cagE, and babA2 Alleles of Helicobacter pylori Bacteria Isolated from Raw Meat

Mashak¹,

Jafariaskari²,

Alavi³

et al. 2020

IDR

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“…By using histopathological examination and real-time PCR-(RT-PCR), gastric biopsy specimens from a southwestern Asian region, Saudi Arabia, were analyzed for vacA, cagA, and iceA genes. 18 The vacA subtypes: s1as1bm2, s1a1b, and s2m2, correlated with chronic gastritis in 90.0%, 8.01%, and 84.2%, respectively. Molecular characterization of the vacA gene in H pylori clinical isolates from patients with gastroduodenal diseases was also carried out in Assam, India.…”

Section: Vaca Caga and Related Virulence Factorsmentioning

confidence: 91%

Review: Pathogenesis of Helicobacter pylori infection

Chmiela

Kupčinskas

2019

Helicobacter

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In this review, we shall focus on the last year progression understanding the pathogenesis of Helicobacter pylori infection in the light of recent data related to adaptation of H pylori to the harsh acidic environment in the stomach, colonization of gastric mucosa via interaction with mucin 5 (MUC5AC) and other host cell receptors, the ability to form biofilm, interference with the host metabolic pathways, and induction of neuroimmune cross‐talk as well as downregulation of gastric barrier homeostasis and its consequences for the disease development. The role of the membrane vesicles of these bacteria has been emphasized as an important source of virulence factors. Furthermore, we shall describe molecular and functional studies on new aspects of VacA and CagA virulence, including the role of urease in the upregulation of VacA toxicity, an epithelial‐mesenchymal transition mediated by CagA, and the role of interaction of HopQ adhesin with carcinoembryonic antigen‐related cell adhesion molecules (CEACAMs) in CagA translocation into the host cells by the type IV secretion system (T4SS). The role of molecular mimicry between a common sequence (ATVLA) of H pylori heat shock protein (Hsp) B and human Hsp60 in the induction of potentially autoreactive antibodies is discussed. All these new data illustrate further progress in understanding H pylori pathogenicity and facilitate the search for new therapeutic targets as well as development of immunoprophylaxis methods based on new chimeric UreB and HpA proteins.

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Role of Bacterial Infection (H. pylori) in Colon Carcinogenesis and Therapeutic Approaches

Singh

Tiwari

Mehdi

et al. 2021

Colon Cancer Diagnosis and Therapy

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Helicobacter pylori vacA, cagA and iceA genotypes in dyspeptic patients from southwestern region, Saudi Arabia: distribution and association with clinical outcomes and histopathological changes

Cited by 37 publications

References 44 publications

<p>Phenotypic and Genotypic Assessment of Antibiotic Resistance and Genotyping of <em>vacA</em>, <em>cagA</em>, <em>iceA</em>, <em>oipA</em>, <em>cagE</em>, and <em>babA2</em> Alleles of <em>Helicobacter pylori</em> Bacteria Isolated from Raw Meat</p>

<p>Phenotypic and Genotypic Assessment of Antibiotic Resistance and Genotyping of <em>vacA</em>, <em>cagA</em>, <em>iceA</em>, <em>oipA</em>, <em>cagE</em>, and <em>babA2</em> Alleles of <em>Helicobacter pylori</em> Bacteria Isolated from Raw Meat</p>

Review: Pathogenesis of Helicobacter pylori infection

Role of Bacterial Infection (H. pylori) in Colon Carcinogenesis and Therapeutic Approaches

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