Helicobacter pylori upregulates circPGD and promotes development of gastric cancer

Abstract

Purpose Helicobacter pylori (H. pylori) has unique biochemical traits and pathogenic mechanisms, which make it a substantial cause of gastrointestinal cancers. Circular RNAs (circRNAs) have concurrently been identified as an important participating factor in the pathophysiology of several different cancers. However, the underlying processes and putative interactions between H. pylori and circRNAs have received very little attention. To address this issue, we explored the interaction between H. pylori and circRNAs to investigate how they might jointly contribute to the occurrence and development of gastric cancer. Methods Changes in circPGD expression in H. pylori were detected using qRT-PCR. Cell proliferation and migration changes were assayed by colony formation, the CCK-8 assay and the transwell assay. Apoptosis was measured by flow cytometry. Western blot was conducted to detect changes in cell migration, apoptosis, proliferation and inflammation-associated proteins. QRT-PCR was used to measure changes in circPGD and inflammation-associated factors. Results We found that H. pylori induced increased circPGD expression in infected human cells and facilitated gastric cancer progression in three ways by promoting cell proliferation and migration, enhancing the inflammatory response, and inhibiting apoptosis. Conclusions CircPGD appears to play a role in H. pylori-related gastric cancer and may thus be a viable, novel target for therapeutic intervention.

Helicobacter pylori Infection in Patients with Gastric Cancer: A 2024 Update

“…[23] For therapeutic intervention, CircPGD might be a promising new target. [24] One potential new target for GC therapy is lnc-PLCB1. [25] Table 2.…”

“…H. pylori promoted cell migration and proliferation, boosted the inflammatory response, and inhibited apoptosis, which led to enhanced circPGD expression in infected human cells and three mechanisms that promoted GC spread. This finding suggested that H. pylori -related GC is influenced by CircPGD [ 24 ]. The formation of GCs is influenced by N6-methyladenosine (m6A) alterations and long noncoding RNAs (lncRNAs).…”