Helicobacter pylori infection and dental care.

Hardo, P G; Tugnait, Aradhna; Hassan, F. M.; Lynch, D. A. F.; West, A. P.; Mapstone, Nicholas P.; Quirke, Philip; Chalmers, D M; Kowolik, Michael; Axon, Anthony

doi:10.1136/gut.37.1.44

Cited by 84 publications

(70 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, successful culture of H. pylori has been difficult in most laboratories. 16,17 Hardo et al 17 using polymerase chain reaction and culture techniques suggested that H. pylori was rarely present in dental plaque in patients with H. pylori gastritis or peptic ulcer disease.…”

Section: Discussionmentioning

confidence: 99%

The prevalence of Helicobacter pylori in practising dental staff and dental students

Lin

Lambert

Schembri

et al. 1998

Australian Dental Journal

View full text Add to dashboard Cite

Recent studies suggest Helicobacter pylori i s spread by faecal-oral or oral-oral transmission. Gastroenterologists who are exposed to gastric secretions and saliva have a high prevalence of H. pylori infection. Venous blood was obtained from 92 dentists, 40 dental nurses, 33 fifth year and 30 first year dental students. An ELISA assay was used to detect H. pylori IgG antibodies. Results were compared with an age and sex matched normal population. The prevalence of H. pylori infection in dentists, dental nurses, fifth year dental students and first year dental students were 23 per cent, 18 per cent, 18 per cent and 16 per cent, respectively. There were no significant differences when compared with the normal population controls. The prevalence of H. pylori antibody was not significantly increased with years of practice or patient contact time in dentists and dental nurses. Helicobacter pylori infection is uncommon in dental professionals working in the oral cavity.

show abstract

Section: Discussionmentioning

confidence: 99%

The prevalence of Helicobacter pylori in practising dental staff and dental students

Lin

Lambert

Schembri

et al. 1998

Australian Dental Journal

View full text Add to dashboard Cite

show abstract

“…This species has been detected in human dental plaque and in whole saliva in numerous studies by culture, PCR and other techniques (Namavar et al, 1995;Song et al, 2000a, b;Young et al, 2001;Allaker et al, 2002), but there are also opposite findings (Asikainen et al, 1994;Wahlfors et al, 1995;Hardo et al, 1995;Luman et al, 1996). This discrepancy can be explained by differences between human populations as well as methodological differences.…”

Section: Introductionmentioning

confidence: 97%

Sensitivity of Helicobacter pylori to an innate defence mechanism, the lactoperoxidase system, in buffer and in human whole saliva

Haukioja

Ihalin

Loimaranta

et al. 2004

Journal of Medical Microbiology

View full text Add to dashboard Cite

Helicobacter pylori has frequently been isolated from human dental plaque, and oral spread via saliva is thought to be one of its principal modes of transmission. Among other innate defence systems human saliva contains peroxidase enzymes and lysozyme. The sensitivity of H. pylori to physiological concentrations of lactoperoxidase and its salivary substrate thiocyanate, and different amounts of hydrogen peroxide (H 2 O 2 ) was investigated in buffer and in human whole saliva. The effect of lysozyme was also studied in saliva. All tested H. pylori strains, ATCC 43504 T and five clinical isolates, were efficiently inhibited by the peroxidase system with high concentrations of H 2 O 2 in buffer. The inhibition was stronger at lower pH. However, in human saliva these high concentrations of H 2 O 2 generated less hypothiocyanite, the antibacterial product of the peroxidase system and the effects of the peroxidase system were weaker. Physiological concentration of lysozyme was not bacteriocidal against H. pylori, nor did it enhance the effect of the peroxidase system in saliva. Thus, further studies are needed to enhance the efficacy of peroxidase systems in human saliva to make it more beneficial not only against dental but also against gastric pathogens.

show abstract

“…Most studies have failed to isolate H. pylori by culture from dental plaque of patients undergoing endoscopy . PCR analysis of dental plaque from endoscopy patients has yielded more conflicting data, with H. pylori found frequently in some studies [15,20,21], but absent or found at very low frequency in other studies [16,22]. In the dental plaque of healthy subjects, culture methods have detected H. pylori at high frequency in a single study [23], and PCR has detected the organism at high frequency in only one study [15].…”

Section: Introductionmentioning

confidence: 99%

Identification by PCR of Helicobacter pylori in subgingival plaque of adult periodontitis patients

Riggio¹,

Lennon²

1999

Journal of Medical Microbiology

View full text Add to dashboard Cite

The PCR was used to detect the presence of Helicobacter pylori in subgingival plaque samples from patients with adult periodontitis. Primers based upon the 16s ribosomal RNA (rRNA) gene sequence of H. pylori were used in a single round of PCR to amplify a 295-bp DNA fragment and the identity of the amplified products was confirmed by Southern blot hybridisation to a digoxigenin-labelled H. pylori probe. Further confirmation of product identity was obtained by DNA sequencing of a proportion of the amplified products. The assay was demonstrated to be specific for H. pylori with a lower limit of detection of 100 fg of bacterial genomic DNA. In all, 73 samples from 29 patients were analysed, of which 24 (33%) were H. pylori-positive by PCR; the proportion of patients carrying H. pylori in at least one sampled site was 38% (11 of 29). This is the first study to demonstrate the presence of H. pylori in the subgingival plaque of patients with adult periodontitis and indicates that, in this patient group at least, subgingival plaque may be a reservoir for H. pylori infection.

show abstract

Helicobacter pylori infection and dental care.

Cited by 84 publications

References 12 publications

The prevalence of Helicobacter pylori in practising dental staff and dental students

The prevalence of Helicobacter pylori in practising dental staff and dental students

Sensitivity of Helicobacter pylori to an innate defence mechanism, the lactoperoxidase system, in buffer and in human whole saliva

Identification by PCR of Helicobacter pylori in subgingival plaque of adult periodontitis patients

Contact Info

Product

Resources

About