Helicobacter pylori-derived outer membrane vesicles suppress liver autophagy: A novel mechanism for H. pylori-mediated hepatic disorder

Shegefti, Saina; Bolori, Shahin; Nabavi-Rad, Ali; Dabiri, Hossein; Yadegar, Abbas; Baghaei, Kaveh

doi:10.1016/j.micpath.2023.106319

Cited by 3 publications

(1 citation statement)

References 41 publications

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“…Although researchers typically use the Bradford [75,76] or BCA methods [77,78], these require polypropylene tubes and 96-well microplates. When measuring protein concentrations, 100-1000 µL of a sample is required, comprising cell lysate/tissue lysate and Bradford or BCA reagents.…”

Section: Previous and Potential Technologies For The Identification A...mentioning

confidence: 99%

Potential of the Novel Slot Blot Method with a PVDF Membrane for Protein Identification and Quantification in Kampo Medicines

Takata,

Masauji,

Motoo

2023

Membranes

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Kampo is a Japanese traditional medicine modified from traditional Chinese medicine. Kampo medicines contain various traditional crude drugs with unknown compositions due to the presence of low-molecular-weight compounds and proteins. However, the proteins are generally rare and extracted with high-polarity solvents such as water, making their identification and quantification difficult. To develop methods for identifying and quantifying the proteins in Kampo medicines, in the current study we employ previous technology (e.g., column chromatography, electrophoresis, and membrane chromatography), focusing on membrane chromatography with a polyvinylidene difluoride (PVDF) membrane. Moreover, we consider slot blot analysis based on the principle of membrane chromatography, which is beneficial for analyzing the proteins in Kampo medicines as the volume of the samples is not limited. In this article, we assess a novel slot blot method developed in 2017 and using a PVDF membrane and special lysis buffer to quantify advanced glycation end products-modified proteins against other slot blots. We consider our slot blot analysis superior for identifying and quantifying proteins in Kampo medicines compared with other methods as the data obtained with our novel slot blot can be shown with both error bars and the statistically significant difference, and our operation step is simpler than those of other methods.

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Section: Previous and Potential Technologies For The Identification A...mentioning

confidence: 99%

Potential of the Novel Slot Blot Method with a PVDF Membrane for Protein Identification and Quantification in Kampo Medicines

Takata,

Masauji,

Motoo

2023

Membranes

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show abstract

The modulatory effect of Lactobacillus gasseri ATCC 33323 on autophagy induced by extracellular vesicles of Helicobacter pylori in gastric epithelial cells in vitro

Sadeghloo,

Saffarian,

Hakemi-Vala

et al. 2024

Microbial Pathogenesis

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Effects of bacterial extracellular vesicles derived from oral and gastrointestinal pathogens on systemic diseases

Zhang,

Lin,

et al. 2024

Microbiological Research

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Helicobacter pylori-derived outer membrane vesicles suppress liver autophagy: A novel mechanism for H. pylori-mediated hepatic disorder

Cited by 3 publications

References 41 publications

Potential of the Novel Slot Blot Method with a PVDF Membrane for Protein Identification and Quantification in Kampo Medicines

Potential of the Novel Slot Blot Method with a PVDF Membrane for Protein Identification and Quantification in Kampo Medicines

The modulatory effect of Lactobacillus gasseri ATCC 33323 on autophagy induced by extracellular vesicles of Helicobacter pylori in gastric epithelial cells in vitro

Effects of bacterial extracellular vesicles derived from oral and gastrointestinal pathogens on systemic diseases

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