Helicobacter pylori-antigen-binding fragments expressed on the filamentous M13 phage prevent bacterial growth

Bacteriophages: an appraisal of their role in the treatment of bacterial infections

“…The filamentous phage M13, which normally has E. coli as its host, was modified using phage display techniques to bind Helicobacter pylori [70]. Antigen-binding single chain variable fragments from murine hybridomas that produced monoclonal antibodies against H. pylori were expressed as a g3p fusion protein on the M13 phage.…”

Bacteriophage therapy: a revitalized therapy against bacterial infectious diseases

“…They contain double-stranded or single-stranded DNA or RNA as the genome. Although most therapeutic phages are tailed, some cubic phages (fX174 and Qb) [35][36][37] or filamentous phages (M13 and Pf3) 38,39 have also been used (see later in this article).…”

“…Furthermore, oral administration of the phage decreased the number of bacteria colonies in the stomachs of mice. 38 The release of endotoxin (lipopolysaccharide), a component of the outer membrane in gram-negative bacteria, by phage infection is thought to be an important problem in phage therapy. Recently, a unique method to minimize the release of endotoxin in phage therapy against P. aeruginosa disease was reported.…”

Phage Therapy and Photodynamic Therapy: Low Environmental Impact Approaches to Inactivate Microorganisms in Fish Farming Plants

“…After this, many successful results using animal models were showed. The therapeutic efficacy of phage therapy against infectious diseases caused by Pseudomonas aeruginosa [149,150], Staphylococcus aureus (including methicillin-resistant S. aureus ) [151,152], E. coli [147], Enterococcus faecium (including vancomycin-resistant Enterococcus ) [153], Streptococcus pneumoniae [154], Helicobacter pylori [155], Klebsiella pneumoniae [156] and Salmonella enteritidis [157,158] has been shown in experimental animal models. With regards to human health, in the past, phage was administered in Poland and the Soviet Union orally, tropically or systemically to treat a wide variety of infections (suppurative wound, gastroenteritis, sepsis, osteomyelitis, dermatitis, emphysemas and pneumonia) in both adults and children [146].…”