Helicobacter nemestrinae ATCC 49396T is a strain of Helicobacter pylori (Marshall et al. 1985) Goodwin et al. 1989, and Helicobacter nemestrinae Bronsdon et al. 1991 is therefore a junior heterotypic synonym of Helicobacter pylori.

Suerbaum, Sebastian; Kraft, Christian; Dewhirst, Floyd E.; Fox, James G.

doi:10.1099/00207713-52-2-437

Cited by 29 publications

(16 citation statements)

References 7 publications

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“…As the G+C content was markedly lower than all other known Helicobacter species (24 mol%) a re-appraisal was undertaken. Sequence analysis of seven housekeeping genes and two flagellin genes from the single strain of H. nemestrinae (ATCC 49396 T ) showed that all sequences clustered with those from multiple isolates of H. pylori (Suerbaum et al 2002). Repeat sequence analysis of the 16S rRNA gene of H. nemestrinae showed that it differed by 38 bp from that previously reported and was less than 1 % different from multiple H. pylori strains.…”

Section: Helicobacter Nemestrinaementioning

confidence: 82%

“…However, 16S rDNA analysis may not differentiate closely related strains or species within the Helicobacter genus due to their high sequence similarity. Moreover, analysis based on the 16S rRNA gene does not necessarily correspond to the results of polyphasic taxonomy Vandamme et al 2000;Suerbaum et al 2002;O'Rourke et al 2004). Indeed, gene analysis of the 60 kDa heat-shock protein (HSP60) has been demonstrated to have a higher resolution than the conventional 16S rRNA gene for species identification of gastric and enterohepatic Helicobacter species (Mikkonen et al 2004).…”

Section: Molecular Analyses Phylogenymentioning

confidence: 95%

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The Family Helicobacteraceae

et al. 2014

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Section: Helicobacter Nemestrinaementioning

confidence: 82%

Section: Molecular Analyses Phylogenymentioning

confidence: 95%

The Family Helicobacteraceae

et al. 2014

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“…Basic nucleotide BLAST alignment (Blastn) of this sequence demonstrated complete homology with 74 H. pylori strains, two H. nemestrinae and four Helicobacter sp. “liver” (that were subsequently found to be indistinguishable from H. pylori [26], [27]), and 17 uncultured Helicobacter species. Sequences of these uncultured Helicobacter species had been determined from biopsies from human esophageal carcinoma or inflamed colon [28], from the stomach of cheetahs [a carnivore that is frequently colonized by the closest H. pylori relative, H. acinonychis [29]], or from the stomach of thoroughbred horses [30].…”

Section: Resultsmentioning

confidence: 99%

Specific and Sensitive Detection of H. pylori in Biological Specimens by Real-Time RT-PCR and In Situ Hybridization

et al. 2008

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PCR detection of H. pylori in biological specimens is rendered difficult by the extensive polymorphism of H. pylori genes and the suppressed expression of some genes in many strains. The goal of the present study was to (1) define a domain of the 16S rRNA sequence that is both highly conserved among H. pylori strains and also specific to the species, and (2) to develop and validate specific and sensitive molecular methods for the detection of H. pylori. We used a combination of in silico and molecular approaches to achieve sensitive and specific detection of H. pylori in biologic media. We sequenced two isolates from patients living in different continents and demonstrated that a 546-bp domain of the H. pylori 16S rRNA sequence was conserved in those strains and in published sequences. Within this conserved sequence, we defined a 229-bp domain that is 100% homologous in most H. pylori strains available in GenBank and also is specific for H. pylori. This sub-domain was then used to design (1) a set of high quality RT-PCR primers and probe that encompassed a 76-bp sequence and included at least two mismatches with other Helicobacter sp. 16S rRNA; and (2) in situ hybridization antisense probes. The sensitivity and specificity of the approaches were then demonstrated by using gastric biopsy specimens from patients and rhesus monkeys. This H. pylori-specific region of the 16S rRNA sequence is highly conserved among most H. pylori strains and allows specific detection, identification, and quantification of this bacterium in biological specimens.

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“…On occasion, descriptions have been controversial [6][7][8][9], and on several occasions the ICSP Subcommittee on the Taxonomy of Campylobacter and Related Bacteria have remarked on the sometimes questionable quality of particular species descriptions that were observed [10][11][12]. Furthermore, taxonomic methods have undergone a 'sea change' with whole-…”

Section: Introductionmentioning

confidence: 99%

Minimal standards for describing new species belonging to the families Campylobacteraceae and Helicobacteraceae: Campylobacter, Arcobacter, Helicobacter and Wolinella spp.

Miller

Houf

et al. 2017

International Journal of Systematic and Evolutionary Microbiology

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Ongoing changes in taxonomic methods, and in the rapid development of the taxonomic structure of species assigned to the Epsilonproteobacteria have lead the International Committee of Systematic Bacteriology Subcommittee on the Taxonomy of Campylobacter and Related Bacteria to discuss significant updates to previous minimal standards for describing new species of Campylobacteraceae and Helicobacteraceae. This paper is the result of these discussions and proposes minimum requirements for the description of new species belonging to the families Campylobacteraceae and Helicobacteraceae, thus including species in Campylobacter, Arcobacter, Helicobacter, and Wolinella. The core underlying principle remains the use of appropriate phenotypic and genotypic methods to characterise strains sufficiently so as to effectively and unambiguously determine their taxonomic position in these families, and provide adequate means by which the new taxon can be distinguished from extant species and subspecies. This polyphasic taxonomic approach demands the use of appropriate reference data for comparison to ensure the novelty of proposed new taxa, and the recommended study of at least five strains to enable species diversity to be assessed. Methodological approaches for phenotypic and genotypic (including whole-genome sequence comparisons) characterisation are recommended.

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Helicobacter nemestrinae ATCC 49396T is a strain of Helicobacter pylori (Marshall et al. 1985) Goodwin et al. 1989, and Helicobacter nemestrinae Bronsdon et al. 1991 is therefore a junior heterotypic synonym of Helicobacter pylori.

Abstract: NOTE

Cited by 29 publications

References 7 publications

The Family Helicobacteraceae

The Family Helicobacteraceae

Specific and Sensitive Detection of H. pylori in Biological Specimens by Real-Time RT-PCR and In Situ Hybridization

Minimal standards for describing new species belonging to the families Campylobacteraceae and Helicobacteraceae: Campylobacter, Arcobacter, Helicobacter and Wolinella spp.

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