Helcococcus kunzii and Helcococcus ovis isolated in dairy cows with puerperal metritis

Locatelli, Clinio; Scaccabarozzi, L.; Pisoni, G.; Bronzo, V.; Casula, A.; Testa, Francesco; Allodi, S.; Pollera, C.; Toni, F.; Moroni, P.

doi:10.2323/jgam.59.371

Cited by 20 publications

(21 citation statements)

References 18 publications

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“…In our network analysis, Lachnospiraceae, Ruminococcaceae , and Bacteroidetes S24-7 demonstrated positive correlation, potentially because all of them are short chain fatty acid (SCFA)-producing strains ( Biddle et al, 2013 ; Ormerod et al, 2016 ). Growing evidence suggests that Helcococcus is involved in a wide spectrum of animal diseases, such as subclinical mastitis and puerperal metritis ( Oikonomou et al, 2012 ; Locatelli et al, 2013 ). In our study, Helcococcus negatively interacted with many potential probiotics, such as Lactobacillus, Oscillospira ( Mackie et al, 2003 ), Ruminococcaceae, Bacteroidales ( Mazmanian et al, 2005 ), and Bacteroidetes S24-7 , while positively correlating with other pathogens, such as Gemella ( Borro et al, 2014 ) and Peptoniphilus ( Brown et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Lactation Stage-Dependency of the Sow Milk Microbiota

Chen

et al. 2018

Front. Microbiol.

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Breast milk is essential for the initial development of neonatal animals, as it provides not only essential nutrients and a broad range of bioactive compounds but also commensal bacteria. The milk microbiota contributes to the “initial” intestinal microbiota of infants and also plays a crucial role in modulating and influencing neonatal health. However, the milk microbiota of sows has yet to be systematically investigated. The goal of the present study was to characterize variations in bacterial diversity and composition in sow milk over the duration of lactation using a high-throughput sequencing approach. Milk samples (n = 160) were collected from 20 healthy sows at eight different time points, and microbial profiles were analyzed by 16S ribosomal RNA (rRNA) sequencing using the Illumina MiSeq platform. The composition and diversity of the milk microbiota changed significantly in colostrum but was relatively stable in transitional and mature milk. Firmicutes and Proteobacteria were the most dominant phyla in sow milk. The relative abundances of the two most dominant bacterial genera, Corynebacterium and Streptococcus, were significantly higher in colostrum than in transitional milk and mature milk samples, and the other four most dominant bacterial taxa (Lactobacillus, two unclassified genera in the families Ruminococcaceae and Lachnospiraceae, and an unclassified genus in the order Clostridiales) demonstrated higher relative abundances in transitional and mature milk than in colostrum. Membrane transport, amino acid metabolism and carbohydrate metabolism were the most abundant functional categories in sow milk communities. Microbial network analysis based on the predominant genera revealed that the abundance of Helcococcus was negatively correlated with the abundances of most other genera in sow milk. Our results are the first to systematically indicate that the sow milk microbiota is a dynamic ecosystem in which changes mainly occur in the colostrum and remain generally stable throughout lactation.

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Section: Discussionmentioning

confidence: 99%

Lactation Stage-Dependency of the Sow Milk Microbiota

Chen

et al. 2018

Front. Microbiol.

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“…To date, four species have been described: Helcococcus kunzii (1993) (1), Helcococcus ovis (1999) (3), Helcococcus sueciensis (2004) (4), and Helcococcus seattlensis (2014) (5). H. ovis has been described from different animals but never from humans (3,(6)(7)(8)(9)(10)(11). H. sueciensis has been isolated only in humans from two wound infections and one bacteremia infection (4,5).…”

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confidence: 99%

Identification and Clinical Significance of Helcococcus kunzii in Human Samples

Vergne¹,

Guérin²,

Lienhard³

et al. 2015

J Clin Microbiol

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“…Biochemical methods are not reliable for H. ovis identification. Several studies have reported the misidentification of H. ovis as Granulicatella adiacens by various biochemical methods [ 12 , 16 , 17 ] . In this particular case, phenotypic characterization using the Vitek2 GP system gave an inconclusive result.…”

Section: Discussionmentioning

confidence: 99%

“…All species, with the exception of H. ovis, have been isolated from human specimens [ 3 – 8 ], and H. kunzii is the most common pathogen [ 7 – 10 ]. H. ovis was first isolated from a mixed infection in sheep in 1999 and was subsequently reported in bovine, horses and goats [ 2 ], but has never been isolated from human specimens, even as a result of foreign body invasion [ 11 , 12 ]. In this report, we describe the first known human case of artificial eye infection, which H. ovis was isolated from the artificial eye .…”

Section: Introductionmentioning

confidence: 99%

Helcococcus ovis in a patient with an artificial eye: a case report and literature review

Chen

et al. 2018

BMC Infect Dis

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BackgroundHelcococcus ovis, belonging to the genus of Helcococcu in Peptostreptococcaceae, is one kind of facultative anaerobic and gram-positive cocci, which was first isolated from a mixed infection in sheep in 1999. To our knowledge, it’s known as an invasive pathogen in animals, and never been reported as a human pathogen in published literature. The aims of this work are to describe the first report of H. ovis which was recovered from the artificial eye of human case and perform a literature review.Case presentationA 26 year-old man reporting pyogenic infection with an artificial eye attended ophthalmic ward in Tongji hospital. After physical examination, clinical and laboratory investigations, the diagnosis of eye infection caused by Helcococcus ovis and Staphylococcus aureus was established. Receiving a medico-surgical approach, the patient was successfully treated. The treatment consisted in intravenous cefotaxime and ornidazole, levofloxacin eye drops during two weeks and removing of right artificial eye with debridement.ConclusionsWe describe here the first known case of H. ovis which was recovered from human artificial eye. This report different from previous data found in the literature emphasizes the invasive potential of this bacterial species as a pathogen in human. Prospectively, the application of next generation sequencing tools would contribute to a more accurate classification of clinical strains.Electronic supplementary materialThe online version of this article (10.1186/s12879-018-3310-7) contains supplementary material, which is available to authorized users.

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Helcococcus kunzii and Helcococcus ovis isolated in dairy cows with puerperal metritis

Cited by 20 publications

References 18 publications

Lactation Stage-Dependency of the Sow Milk Microbiota

Lactation Stage-Dependency of the Sow Milk Microbiota

Identification and Clinical Significance of Helcococcus kunzii in Human Samples

Helcococcus ovis in a patient with an artificial eye: a case report and literature review

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