HeLa cells 50 years on: the good, the bad and the ugly

Masters, J. R. W.

doi:10.1038/nrc775

Cited by 429 publications

(329 citation statements)

References 37 publications

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“…Int407 cells were originally derived from the jejunum and ileum of a 2-month-old Caucasian embryo; however, this cell line was subsequently contaminated by the HeLa cell line (Masters, 2002) and stocks from culture collections supplying Int407s contain HeLa cells. It is therefore classified as a human cervical carcinoma cell line rather than an intestinal epithelial cell line.…”

Section: Discussionmentioning

confidence: 99%

Campylobacter jejuni-infected human epithelial cell lines vary in their ability to secrete interleukin-8 compared to in vitro-infected primary human intestinal tissue

et al. 2006

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Campylobacter jejuni causes symptoms of acute inflammatory diarrhoea in man. C. jejuni interaction with epithelial cells elicits interleukin-8 (IL-8) production, and IL-8 recruits neutrophils to sites of infection. Cell culture models of bacterial interaction with epithelium are useful to define bacteria–host interaction and are used because it is thought they mimic the same bacteria–host cell interaction in the natural disease. This study looks at the ability of C. jejuni strains to elicit IL-8 production from a variety of cell lines previously used for investigating the interaction of C. jejuni with host cells. A spectrum of IL-8 responses was observed, with minimal IL-8 elicited from Caco-2 cells and more marked responses elicited from HeLa and T84 cells. These in vitro-infected cell line responses were compared to IL-8 production from in vitro C. jejuni-infected human colonic and ileal tissue. The in vitro-infected tissue elicited the highest IL-8 responses and the cytokine was manifested earlier compared to the infected cell lines.

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Section: Discussionmentioning

confidence: 99%

Campylobacter jejuni-infected human epithelial cell lines vary in their ability to secrete interleukin-8 compared to in vitro-infected primary human intestinal tissue

et al. 2006

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“…37 The comparison of NR-MSPs uptake in both cervical cancer cells and epithelial cell lines at a specific concentration, i.e. 1 µg mL -1 demonstrated that, while HeLa cells express a much higher level of folate receptors 38 (Fig. S6 †), they internalized much more FA-Chitosan-NR-MSP (~90%), as compared to SiHa cells (Fig.…”

Section: Cell Viabilitymentioning

confidence: 99%

Targeted delivery of a novel anticancer compound anisomelic acid using chitosan-coated porous silica nanorods for enhancing the apoptotic effect

et al. 2015

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Targeted delivery of a novel anticancer compound anisomelic acid using chitosan-coated porous silica nanorods for enhancing the apoptotic effect, 2015, BIOMATERIALS SCIENCE, (3) Targeted cancer therapies are currently a strong focus in biomedical research. The most common approach is to use nanocarrier-based targeting to specifically deliver conventional anticancer drugs to enhance their therapeutic efficacy, increase bioavailabil ity, and decrease the side-effects on normal cells. A step further towards higher specificity and efficacy would be to employ specific novel drugs along with specific nanocarrier -based targeting. Our recent studies have demonstrated that a plant-derived diterpenoid compound, anisomelic acid (AA), induces apoptosis in cervical cancer cells. In this work, we describe the development of a folic acid (FA)-targeted AA delivery system using chitosan-coated rod-shaped mesoporous silica particles (Chitosan-NR-MSP). The cellular internalization and and uptake enhancement of the FA-Chitosan-NR-MSP towards cancerous folate receptor (FR) -positive (SiHa and HeLa) and/or normal FR-negative (HEK 293) cells were assessed, which indicated that the intracellular uptake of FA-conjugated Chitosan-NR-MSP was more target-specific. Furthermore, the induction of apoptosis by AA-loaded chitosan-coated rod-shaped particles on SiHa cells was studied. By employing caspase activation and PARP cleavage as measure of apoptosis, the FA-particle mediated AA treatment was clearly more effective, significantly enhancing apoptosis in comparison to non-targeted Chitosan-NR-MSP or free AA in SiHa cells, suggesting that the FA-Chitosan-NR-MSPs can be potentially utilized as a drug delivery system for cervical cancer treatment.

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“…Lung cancer cells were successfully cultured approximately 25 years after the establishment of a human cervical carcinoma (HeLa) cell line [3,4] . Since then, many attempts have been made to establish permanent lung cancer cell lines [5][6][7][8][9][10] .…”

Section: Introductionmentioning

confidence: 99%

Establishment and characterization of primary lung cancer cell lines from Chinese population

Zheng

Sun

et al. 2011

Acta Pharmacol Sin

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Aim: To establish and characterize primary lung cancer cell lines from Chinese population. Methods: Lung cancer specimens or pleural effusions were collected from Chinese lung cancer patients and cultured in vitro with ACL4 medium (for non-small cell lung carcinomas (NSCLC)) or HITES medium (for small cell lung carcinomas (SCLC)) supplemented with 5% FBS. All cell lines were maintained in culture for more than 25 passages. Most of these cell lines were further analyzed for oncogenic mutations, karyotype, cell growth kinetics, and tumorigenicity in nude mice. Results: Eight primary cell lines from Chinese lung cancer patients were established and characterized, including seven NSCLC cell lines and one SCLC cell line. Five NSCLC cell lines were found to harbor epidermal growth factor receptor (EGFR) kinase domain mutations. Conclusion: These well-characterized primary lung cancer cell lines from Chinese population provide a unique platform for future studies of the ethnic differences in lung cancer biology and drug response.

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HeLa cells 50 years on: the good, the bad and the ugly

Cited by 429 publications

References 37 publications

Campylobacter jejuni-infected human epithelial cell lines vary in their ability to secrete interleukin-8 compared to in vitro-infected primary human intestinal tissue

Campylobacter jejuni-infected human epithelial cell lines vary in their ability to secrete interleukin-8 compared to in vitro-infected primary human intestinal tissue

Targeted delivery of a novel anticancer compound anisomelic acid using chitosan-coated porous silica nanorods for enhancing the apoptotic effect

Establishment and characterization of primary lung cancer cell lines from Chinese population

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