Hedgehog signalling maintains the optic stalk-retinal interface through the regulation of Vax gene activity

Takeuchi, Masaru; Clarke, Jon; Wilson, Stephen W.

doi:10.1242/dev.00305

Cited by 159 publications

(180 citation statements)

References 58 publications

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“…These markers include dorsally: ephrinB2A (25), msx-c (26), tbx5.1 (27), and BMP4 (28), and ventrally: pax2a (29) and vax2 (30). Whereas the direct functions of most of these molecules have yet to be well characterized within the retina, they are thought to play a role in compartment patterning, and serve as useful markers for the gross subdivision of the retina into dorsal and ventral compartments.…”

Section: Resultsmentioning

confidence: 99%

Tbx2b is essential for neuronal differentiation along the dorsal/ventral axis of the zebrafish retina

Gross

Dowling

2005

Proc. Natl. Acad. Sci. U.S.A.

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The mechanisms by which retinal neurons are patterned along the dorsal͞ventral axis remain largely unknown, yet this patterning is integral for the topographic mapping of visual space. With an interest in elucidating the mechanisms that regulate the development of this retinal axis, we have characterized a T-box family transcription factor, Tbx2b, during zebrafish retinogenesis. Tbx2b is expressed throughout all phases of retinal development with a striking asymmetry of distribution highest dorsally to lowest ventrally. To examine Tbx2b function during retinal development, two morpholino antisense oligonucleotides were created; one blocking the translational start site of Tbx2b and the other interfering with Tbx2b mRNA splicing. Injection of either of these morpholinos resulted in profound defects in the development of the dorsal retina. By using molecular markers for neuronal subtypes, the ventral retina contained all cell types, whereas in the dorsal retina, only retinal ganglion cells expressed markers of differentiation. The cells of the dorsal retina were postmitotic, however, as demonstrated by a lack of BrdUrd incorporation during the normal periods of retinal differentiation. Markers for dorsal and ventral retinal compartments were also expressed normally in Tbx2b morphants. Combined, these observations suggest that the cellular mechanisms regulating neuronal differentiation within the retina are asymmetric about the dorsal͞ventral axis and that Tbx2b mediates this process within the dorsal retina.T-box ͉ retinal development

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Section: Resultsmentioning

confidence: 99%

Tbx2b is essential for neuronal differentiation along the dorsal/ventral axis of the zebrafish retina

Gross

Dowling

2005

Proc. Natl. Acad. Sci. U.S.A.

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“…Liu et al, 2001Shen and Raymond, 2004 Boucher and Hitchcock, 1998a;Mack and Fernald, 1993;Otteson et al, 2002FGFs Heisenberg et al, 1999Picker andBrand, 2005 Mack andMartinez-Morales et al, 2005Wnts Masai et al, 2005Yamaguchi et al, 2005Hh Macdonald et al, 1995Stenkamp and Frey, 2003a;Take-uchi et al, 2003Masai et al, 2005Shkumatava and Neumann, 2005;Masai et al, 2005Neumann and Nuesslein-Volhard, 2000;Shkumatava et al, 2004;Stenkamp and Frey, 2003a;Stenkamp et al, 2000RA Hyatt et al, 1992, 1996bMarsh-Armstrong et al, 1994Biehlmaier et al, 2005Hyatt et al, 1996a;Prabhudesai et al, 2005Notch Yamaguchi et al, 2005Bernardos et al, 2005Scheer et al, 2001Cadherins Babb et al, 2005Malicki et al, 2003 …”

Section: Tablementioning

confidence: 99%

Neurogenesis in the Fish Retina

Stenkamp

2007

International Review of Cytology

122

136

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The retinas of teleost fish have long been of interest to developmental neurobiologists for their persistent plasticity during growth, life history changes, and response to injury. Because the vertebrate retina is a highly conserved tissue, the study of persistent plasticity in teleosts has provided insights into mechanisms for postembryonic retinal neurogenesis in mammals. In addition, in the past 10 years there has been an explosion in the use of teleost fish-zebrafish (Danio rerio) in particular-to understand the mechanisms of embryonic retinal neurogenesis in a model vertebrate with genetic resources. This review summarizes the key features of teleost retinal neurogenesis that make it a productive and interesting experimental system, and focuses on the contributions to our knowledge of retinal neurogenesis that uniquely required or significantly benefited from the use of a fish model system.

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“…Cell labeling (caged fluorophore) Fate determination for a specific group of cells Take-uchi et al (2003) Cell labeling (iontophoretic) Determination of morphogenetic movements or cell lineage relationships Li et al (2000b), Varga et al (1999), Woo and Fraser (1995) Cell labeling (lipophilic tracers) Analysis of ganglion cell development (for example, retino tectal projection) Baier et al (1996), Malicki and Driever (1999) Blue cones (≤3 dpf) Doerre and Malicki (2001), Vihtelic et al (1999) Blue opsin c…”

Section: Embryological Techniquesmentioning

confidence: 99%

Analysis of the retina in the zebrafish model

Malicki

Pooranachandran

Nikolaev

et al. 2016

Methods in Cell Biology

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Hedgehog signalling maintains the optic stalk-retinal interface through the regulation of Vax gene activity

Abstract: During early formation of the eye, the optic vesicle becomes partitioned into a proximal domain that forms the optic nerve and a distal domain that forms the retina. In this study, we investigate the activity of Nodal, Hedgehog (

Cited by 159 publications

References 58 publications

Tbx2b is essential for neuronal differentiation along the dorsal/ventral axis of the zebrafish retina

Tbx2b is essential for neuronal differentiation along the dorsal/ventral axis of the zebrafish retina

Neurogenesis in the Fish Retina

Analysis of the retina in the zebrafish model

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