Hedgehog Creates a Gradient of DPP Activity in Drosophila Wing Imaginal Discs

Tanimoto, Hiromu; Itoh, Shinji; Dijke, Peter ten; Tabata, Tetsuya

doi:10.1016/s1097-2765(00)80403-7

Cited by 381 publications

(380 citation statements)

References 66 publications

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“…In the wild-type wing discs, pMad levels are high in the center, and then graded towards the peripheral region of the wing pouch as observed by immunostaining (Figure 1A), consistent with other’s reports [54]. Over expression of ban along the A/P boundary in the wing imaginal disc by ptc-Gal4 resulted in greatly decreased pMad levels along the A/P boundary in these animals (Figure 1B).…”

Section: Resultssupporting

confidence: 90%

bantam microRNA is a negative regulator of the Drosophila decapentaplegic pathway

Kane

Vora

Padgett

et al. 2018

Fly

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Decapentaplegic (Dpp), the Drosophila homolog of the vertebrate bone morphogenetic protein (BMP2/4), is crucial for patterning and growth in many developmental contexts. The Dpp pathway is regulated at many different levels to exquisitely control its activity. We show that bantam (ban), a microRNA, modulates Dpp signaling activity. Over expression of ban decreases phosphorylated Mothers against decapentaplegic (Mad) levels and negatively affects Dpp pathway transcriptional target genes, while null mutant clones of ban upregulate the pathway. We provide evidence that dpp upregulates ban in the wing imaginal disc, and attenuation of Dpp signaling results in a reduction of ban expression, showing that they function in a feedback loop. Furthermore, we show that this feedback loop is important for maintaining anterior-posterior compartment boundary stability in the wing disc through regulation of optomotor blind (omb), a known target of the pathway. Our results support a model that ban functions with dpp in a negative feedback loop.

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Section: Resultssupporting

confidence: 90%

bantam microRNA is a negative regulator of the Drosophila decapentaplegic pathway

Kane

Vora

Padgett

et al. 2018

Fly

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“…Because GSCs require a Dpp signal to maintain their stem cell identity, we reasoned that the niche can be no larger that the region capable of stimulating a competent germ cell to activate its Dpp pathway. Immunostaining of wild-type ovarioles with pMAD (22) revealed that only GSCs and possibly CBs activate the Dpp pathway at the highest levels (Fig. 1c, arrowhead).…”

Section: Resultsmentioning

confidence: 99%

An empty Drosophila stem cell niche reactivates the proliferation of ectopic cells

Kai

Spradling

2003

Proc. Natl. Acad. Sci. U.S.A.

255

216

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Stem cells are thought to reside in regulatory microenvironments (“niches”) generated by stable stromal neighbors. To investigate the significance of empty niches vacated by stem cell loss, we studied Drosophila ovarioles, which maintain two to three germ-line stem cells in a niche requiring adhesive stromal cap cells and Decapentaplegic signals. After experimentally emptying the germ-line stem cell niche, cap cell activity persists for several weeks. Initially, somatic inner germarium sheath cells enter the empty niche, respond to Dpp, but fail to divide. Subsequently, follicle cell progenitors, including somatic stem cells enter the niche, respond to Dpp, and proliferate as long as cap cells remain. Proliferation requires the normal hedgehog signal of the somatic stem cells as well as proximity to the niche. Thus, empty niches can persist, signal incoming cells, and support ectopic proliferation. Similar events may underlie some disease states

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“…hh-Gal4, hh-nlsDsRed and ptc-Gal4 enhancer trap lines express in wing discs in patterns that mimic hh and ptc, respectively (Akimoto et al, 2005;RamirezWeber et al, 2000;Tanimoto et al, 2000). Stocks were obtained from Bloomington Drosophila Stock Center (BDSC): UAS-disp RNAi (#27247), UAS-dia RNAi (#28541), tub-Gal80 ts (#7018), UAS-CD8:GFP (#5137), yw, ubi-RFP.nls FRT40A/CyO (#34500) and UAS-Nrg RNAi (#37496).…”

Section: Drosophila Stocksmentioning

confidence: 99%

Essential basal cytonemes take up Hedgehog in the Drosophila wing imaginal disc

et al. 2017

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Morphogen concentration gradients that extend across developmental fields form by dispersion from source cells. In the Drosophila wing disc, Hedgehog (Hh) produced by posterior compartment cells distributes in a concentration gradient to adjacent cells of the anterior compartment. We monitored Hh:GFP after pulsed expression, and analyzed the movement and colocalization of Hh, Patched (Ptc) and Smoothened (Smo) proteins tagged with GFP or mCherry and expressed at physiological levels from bacterial artificial chromosome transgenes. Hh:GFP moved to basal subcellular locations prior to release from posterior compartment cells that express it, and was taken up by basal cytonemes that extend to the source cells. Hh and Ptc were present in puncta that moved along the basal cytonemes and formed characteristic apical-basal distributions in the anterior compartment cells. The basal cytonemes required diaphanous, SCAR, Neuroglian and Synaptobrevin, and both the Hh gradient and Hh signaling declined under conditions in which the cytonemes were compromised. These findings show that in the wing disc, Hh distributions and signaling are dependent upon basal release and uptake, and on cytoneme-mediated movement. No evidence for apical dispersion was obtained.

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Hedgehog Creates a Gradient of DPP Activity in Drosophila Wing Imaginal Discs

Cited by 381 publications

References 66 publications

bantam microRNA is a negative regulator of the Drosophila decapentaplegic pathway

bantam microRNA is a negative regulator of the Drosophila decapentaplegic pathway

An empty Drosophila stem cell niche reactivates the proliferation of ectopic cells

Essential basal cytonemes take up Hedgehog in the Drosophila wing imaginal disc

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