Heavy water enhancement of fluorescence signal in reversed-phase liquid chromatography

Táborský, Petr; Kučera, Josef; Juřica, Jan; Peš, Ondřej

doi:10.1016/j.jchromb.2018.05.037

Cited by 4 publications

(4 citation statements)

References 28 publications

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“…This has been illustrated several times by enhanced fluorescence intensity and FLT in D2O. [62][63][64][65] The exact mechanism by which this quenching occurs and what parameters affect it is still to be fully understood and it is thus difficult to predict the extent of the effect.…”

Section: Intrinsic Water Quenchingmentioning

confidence: 99%

What is Best Strategy for Water Soluble Fluorescence Dyes?—A Case Study Using Long Fluorescence Lifetime DAOTA Dyes**

Bisballe

Laursen

2020

Chemistry A European J

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The lipophilic natureo fo rganic dyes complicates their effectiveness in aqueous solutions. In this work we investigate threed ifferent strategies for achieving water-solubility of the diazaoxatriangulenium (DAOTA +)c hromophore: hydrophilic counter ions, aromatic sulfonationo ft he chromophore, and attachment of charged side chains. The long fluorescence lifetime(FLT, t f = 20 ns) of DAOTA + makes it a sensitive probet oa nalyze solvation and aggregation effects. Direct sulfonation of the chromophore was found to increase solubility drastically,b ut at the cost of greatly re-ducedq uantum yields (QYs) due to enhanced non-radiative deactivation processes. The introduction of either cationic (4) or zwitterionic side chains (5), however,b rings the FLT (t f = 18 ns) and QY (f f = 0.56) of the dye to the same level as the parent chromophorei na cetonitrile. Time-resolved fluorescence spectroscopya lso reveals ah igh resistance to aggregation and non-specific binding in ah igh loading of bovines erum albumin (BSA). The results clearly show that addition of charged flexibles ide chains is preferable to directs ulfonation of the chromophore core.

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Section: Intrinsic Water Quenchingmentioning

confidence: 99%

What is Best Strategy for Water Soluble Fluorescence Dyes?—A Case Study Using Long Fluorescence Lifetime DAOTA Dyes**

Bisballe

Laursen

2020

Chemistry A European J

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“…114 In a very practical application, Pes and co-workers utilized D 2 O as an elution solvent in high performance liquid chromatography (HPLC) to facilitate the fluorescence detection of such compounds as doxorubicin and fluorescein. 115 When small molecule Schiff base crystals were created, 116 they became fluorescent in the presence of water and even more so in D 2 O. After studying the deuterium fluorescence enhancement in this system, the authors proposed that the chemistry could also be used to detect and quantitate D 2 O content in water.…”

Section: Other Examples Of Deuterium Luminescence Enhancementmentioning

confidence: 99%

“…The use of D 2 O in luminescence measurements of CdTe and HgTe quantum dots provided valuable information about the degree to which the surrounding solvent participated in their luminescence properties 114 . In a very practical application, Pes and co‐workers utilized D 2 O as an elution solvent in high performance liquid chromatography (HPLC) to facilitate the fluorescence detection of such compounds as doxorubicin and fluorescein 115 . When small molecule Schiff base crystals were created, 116 they became fluorescent in the presence of water and even more so in D 2 O.…”

Section: Other Examples Of Deuterium Luminescence Enhancementmentioning

confidence: 99%

Luminescence enhancement by deuterium

Filer

2023

Labelled Comp Radiopharmac

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Created literally at the dawn of time, deuterium has been extremely valuable in so many chemistry roles. The subject of this review focuses on one deuterium application in particular: its enhancement of luminescence in many substances. After providing general overviews of both deuterium and luminescence, the early exploration of deuterium's effect on luminescence is described, followed by a number of specific topics. These sections include a discussion of deuterium‐influenced luminescence for dyes, proteins, singlet oxygen, and the lanthanide elements, as well as anomalous inverse deuterium luminescence effects. Future directions for this important research topic are also proposed, as well as a summary conclusion.

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“…UV-VIS detection is one of the most popular modes for high performance liquid chromatography, but its application in ion chromatography is limited [35]. The fluorescence detector is rarely used in IC, as only some ions have a fluorescent appearance [36]. Amperometric detection can be used for samples with pK values greater than 7 [37,38].…”

Section: Detectorsmentioning

confidence: 99%

Ion Chromatography: Principles and instrumentation

Moura

Silva

Gubert

2022

Orbital: Electron. J. Chem.

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Ion chromatography (IC) is one of the most used analytical methods for the determination of inorganic cations and anions, as well as organic ions. It is considered a method of separation with simple steps, low cost and with good efficiency in chromatographic separation. In addition, it has good validation metrics such as sensitivity, selectivity, precision, robustness, low detection limit and quantification. IC easily replaces conventional wet chemistry methods, which tend to have more analysis steps. The easy extraction of samples, in most cases, does not require organic solvents, facilitating the disposal of waste. Since its creation, IC has accumulated advantages and good acceptance in the food, pharmaceutical, environmental monitoring industries, in addition to increasing implementation in academic research laboratories.

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Heavy water enhancement of fluorescence signal in reversed-phase liquid chromatography

Cited by 4 publications

References 28 publications

What is Best Strategy for Water Soluble Fluorescence Dyes?—A Case Study Using Long Fluorescence Lifetime DAOTA Dyes**

What is Best Strategy for Water Soluble Fluorescence Dyes?—A Case Study Using Long Fluorescence Lifetime DAOTA Dyes**

Luminescence enhancement by deuterium

Ion Chromatography: Principles and instrumentation

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