Heavy-Paper Technique for Preparative Chromatography

Abstract

A simple method for isolating pure compounds on a gram scale by means of paper chromatography is described. The mixture of compounds to be isolated is applied as a streak to a sheet of heavy paper which is suspended in a chromatographic cabinet. A solvent develops the chromatograms. The resulting resolved bands are located on a print which is made by sandwiching the wet heavy-paper chromatogram, with a sheet of dry Whatman No. 1 paper, between two sheets of glass. The bands are then cut from the heavy paper and isolated by chromatographic extraction.

Large-scale, preparative paper chromatography

“…in th e lin es of absorption, limits of the b a nd s are more clearly de fin ed than in a " print" [3] of th e e ntire c hromatogram . S praying of alternate s tri ps, or of two diffe re nt tracings , with differ ent color reagents so me times gives addition al inform ati on concernin g th e compounds.…”

“…3 (b) Because the seed-test paper is not pure cellulose, it mus t b e chromatographically wash ed with dilute acid a nd with wate r ; thi s treatme nt requires several days. Finally, (c) th e movem e nt of th e bands through the paper is not as uniform as that in papers made of pure cellulose.…”

Organic chemistry :

“…In 1957* Brownell and co-workers [46] described a large-scale, Figure 6. Simplified preparation of stirrup and wick for heavy-paper chromatogram.…”

“…The sheets are air-dried, and strips (l portions of the lines that cross the bands., the limits of the bands are more clearly discerned than in a "print" [46] Structures of 2-0xo-1^3-bis (phenylhydrazono) Compounds" was prepared, and will be submitted for publication in the near future. The paper presents measurements of NMR, infrared., ultraviolet., and visible spectra for twelve phenylhydrazono compounds , prepared to show that 2-0X0-1,3-…”

Clam Poison: Ii. Purification of Clam Poison Residues of Low Toxicity by a Heavy-Paper Technique

“…T o the other 18-in. edge was attached a stirrup and two wicks of Whatman 3MM paper (6). Eight such sheets supported by glass rods in a R4odel A 300 Chromatocab equipped with solvent troughs and reservoirs (6) were washed chromatographically by the procedure described earlier (5) for Whatman No.…”