Heavy metals in isopods from the supra-littoral zone on the Southern shore of the Severn Estuary, UK

Hopkin, S. P.; Martin, Michael; Moss, S.J.

doi:10.1016/0143-148x(85)90001-1

Cited by 35 publications

(13 citation statements)

References 38 publications

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“…For instance, branched chain amino acids (leucine, isoleucine and valine) are involved in muscle growth [36], as well as in haemocyanin synthesis and transportation [37], lysine is related to the production of elastin and collagen [38] and to the absorption of calcium for exoskeleton formation [39], phenylalanine and tyrosine are involved in the synthesis of the pigment melanin and in haemocyanin transportation processes [40], while glutamine is involved in muscle growth [41].Asinother crustaceans, growth and moult are tightly correlated in terrestrial isopods and dependent on the frequent replacement of their cuticle [39]. Although moulting is not considered the main excretion route of metals in these organisms [42], it has been reported to take part on this process. For instance, a previous study has shown that copper-containing granules were able to dissolve during the moult process allowing their excretion [43].…”

Section: Discussionmentioning

confidence: 99%

Metabolic responses of the isopod Porcellionides pruinosus to nickel exposure assessed by 1H NMR metabolomics

Ferreira

Saborano

Morgado

et al. 2016

Journal of Proteomics

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This work aimed at characterizing the metabolome of the isopod Porcellionides pruinosus and at assessing its variations over 14 days under laboratory culture conditions and upon exposure to the contaminant metal Nickel (Ni). The spectral profiles obtained by 1 H NMR spectroscopy were thoroughly assigned and subjected to multivariate analysis in order to highlight consistent changes. Over 50 metabolites could be identified, providing considerable new knowledge on the metabolome of these model organisms. Several metabolites changed non-linearly with Ni dose and exposure time, showing distinct variation patterns for initial (4 days) and later time points (7 and 14 days). In particular, at day 4, several amino acids were increased and sugars were decreased (compared to controls), whereas these variations were inverted for longer exposure, possibly reflecting earlier and more intensive moulting. Other variations, namely in betaines and choline-containing compounds, were suggested to relate with osmoregulation and detoxification mechanisms. Ni also had a marked effect on several nucleotides (increased upon exposure) and a moderate impact on lipids (decreased upon exposure). Overall, this study has provided new information on the Ni-induced metabolic adaptations of the P. pruinosus isopod, paving the way for improved mechanistic understanding of how these model organisms handle soil contamination.Significance: This study provided, for the first time to our knowledge, a detailed picture of the NMR-detectable metabolome of terrestrial isopods and of its fluctuations in time and upon exposure to the contaminant metal Nickel. Several time-and dose-dependent changes were highlighted, providing mechanistic insight into how these important model organisms handle Ni contamination.

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Section: Discussionmentioning

confidence: 99%

Metabolic responses of the isopod Porcellionides pruinosus to nickel exposure assessed by 1H NMR metabolomics

Ferreira

Saborano

Morgado

et al. 2016

Journal of Proteomics

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“…1), is the main tissue for the absorption of nutrients and metals and has shown the highest concentrations of zinc, cadmium, lead, and copper so far recorded in a soft tissue of any terrestrial animal Martin, 1982b, 1984;Donker et al, 1996). The amount of metals stored in the hepatopancreas, however, varies from species to species, and it has been shown that, e.g., Porcellio scaber stores four times as much copper in this organ as Oniscus asellus from the same site (Hopkin et al, 1985a). The metals are stored predominantly in granules in an insoluble form (Patanè, 1934) and, therefore, are largely withdrawn from the cellular metabolism.…”

Section: Woodlice (Isopoda)mentioning

confidence: 99%

“…Wieser (1965) has suggested that copper-containing granules dissolve during molting and allow for copper excretion. But Hopkin et al (1985a) have shown that the copper level in intermolt animals is too high to allow for massive copper loss during molting. Furthermore, the cells containing the copper granules (S-cells) do not undergo a daily cycle (Hames and Hopkin, 1990) and never void their contents into the lumen of the hepatopancreas (Hopkin, 1990).…”

Section: Woodlice (Isopoda)mentioning

confidence: 99%

Localization of metals in cells of saprophagous soil arthropods (Isopoda, Diplopoda, Collembola)

Köhler¹

2002

Microscopy Res & Technique

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This review summarizes results on the intracellular distribution of metals in cells of woodlice (Isopoda), millipedes (Diplopoda), and springtails (Collembola), which are three major groups of saprophagous arthropods contributing to the turnover of soil organic matter. Although the impact of metals and also metal pollution has inevitably been shown at levels of higher biological organization than subcellular mechanisms in these animal groups, the aim of this review is to focus exclusively on storage sites and aspects of intracellular metal metabolism. Thus, methodologically, results obtained by microscopical techniques such as histochemistry, X-ray microanalysis, energy filter transmission electron microscopy, or laser microprobe mass spectrometry were given preference. Results from atomic absorption spectrophotometry of cellular fractions were kept to a minimum. In all three taxa, the main intracellular metal storage sites are various types of "granules" which are widely distributed throughout cell types associated with the digestive system.

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“…Studies on metal storage in woodlice which were collected from the same microhabitat have demonstrated considerable differences between species in the concentrations of some metals in the hepatopancreas. Hopkin et al (1985), for example, showed that despite a lack of significant differences between the concentrations of copper in the hepatopancreas of Oniscus asellus and Porcellio scaber from a site on the shore of the Severn Estuary, UK, there were large and inconsistent differences in the concentration of zinc, cadmium and lead in this organ. Even though the isopods were apparently consuming the same diet, concentrations of zinc in the hepatopancreas of P. scaber were about four times higher than in O. asellus.…”

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confidence: 99%

Energy Reserves and Metal-storage Granules in the Hepatopancreas of Oniscus asellus and Porcellio scaber (Isopoda) from a Metal Gradient at Avonmouth, UK

Schill

Köhler

2004

Ecotoxicology

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Isopods taken from populations of Onsicus asellus and Porcellio scaber from long-term polluted sites in the vicinity of metal smelting works at Avonmouth, South West England, and from a control site near Tübingen, South Germany, were examined for the frequency and size of metal-containing granules (spherites), lipid droplets and glycogen in their hepatopancreas. The number and size of spherites in the hepatopancreas of O. asellus increased with decreasing distance to the smelter, but such a trend was not found in P. scaber. A trend towards massive reduction in hepatopancreatic energy reserves (lipid, glycogen) with increasing soil metal pollution was observed for O. asellus while, in contrast, the lipid and glycogen content of P. scaber midgut gland cells was independent of the distance to the smelter. In view of previous reports on metal accumulation and biochemical responses to metal pollution, we propose that the two investigated isopod species, which form stable populations in the Avonmouth metal gradient, use different strategies to survive.

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Heavy metals in isopods from the supra-littoral zone on the Southern shore of the Severn Estuary, UK

Cited by 35 publications

References 38 publications

Metabolic responses of the isopod Porcellionides pruinosus to nickel exposure assessed by 1H NMR metabolomics

Metabolic responses of the isopod Porcellionides pruinosus to nickel exposure assessed by 1H NMR metabolomics

Localization of metals in cells of saprophagous soil arthropods (Isopoda, Diplopoda, Collembola)

Energy Reserves and Metal-storage Granules in the Hepatopancreas of Oniscus asellus and Porcellio scaber (Isopoda) from a Metal Gradient at Avonmouth, UK

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