Heavy metals and DNA damage in blood cells of insectivore bats in coal mining areas of Catarinense coal basin, Brazil

Zocche, Jairo José; Leffa, Daniela Dimer; Damiani, Adriani Paganini; Carvalho, Fernando; Mendonça, Rodrigo Ávila; Santos, Carla Eliete Iochims dos; Boufleur, Liana Appel; Dias, Johnny Ferraz; Andrade, Vanessa Moraes de

doi:10.1016/j.envres.2010.06.003

Cited by 92 publications

(22 citation statements)

References 60 publications

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“…Cadmium injections were linked with testicular necrosis in Rhinopoma kinneari (Dixit and Lohiya, 1974). Microscopic lesions, including hepatic necrosis, vacuolation, inflammation and atrophy, as well as renal necrosis and inflammation, were reported in Neoromicia nana feeding at wastewater treatment sites, with elevated tissue levels of iron, zinc, and copper; other studies of this population documented DNA damage and decreased antioxidant capacity (Naidoo et al, 2013(Naidoo et al, , 2015(Naidoo et al, , 2016Zocche et al, 2010). Organic tin compounds have been associated with decreased complement activity and a possible decrease in immune response in Myotis daubentonii (Lilley et al, 2013).…”

Section: Additional Toxic Diseasesmentioning

confidence: 99%

Chiroptera

Farina

Lankton

2018

Pathology of Wildlife and Zoo Animals

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FIGURE 25.2 Normal patagium, Pteropus vampyrus. The patagium consists of two thin layers of epidermis separated by collagen, vessels, and bundles of elastic fibers. FIGURE 25.1 FIGURE 25.4 Normal brain, Pteropus hypomelanus. The brain is largely lissencephalic, with rare shallow sulci. (Photo Courtesy of E. Kieran, University of Florida) FIGURE 25.5 Iron overload in the liver, Rousettus aegyptiacus. (A) Bridging fibrosis with iron overload. Abundant brown pigment is present within and at the margins of the connective tissue. (B) Abundant hemosiderin is present in macrophages at the margins of and within the fibrous connective tissue, with lesser amounts in hepatocytes, bile ducts, and Kupffer cells. Prussian blue.

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Section: Additional Toxic Diseasesmentioning

confidence: 99%

Chiroptera

Farina

Lankton

2018

Pathology of Wildlife and Zoo Animals

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“…Likewise, Zimmermann (1999) found differences in levels of mtDNA damage between Atlantic tomcod (Microgadus tomcod) from the PAH contaminated Hudson River and a reference site. Although they did not examine mtDNA damage specifically, Zocche et al (2010) use the Comet assay to detect significantly higher levels of genotoxicity in the bat Tadarida brasiliensis at coal mining areas in Brazil than at reference sites.…”

Section: Introductionmentioning

confidence: 99%

Assessment of mitochondrial DNA damage in little brown bats (Myotis lucifugus) collected near a mercury-contaminated river

et al. 2014

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Historical discharges of Hg into the South River near the town of Waynesboro, VA, USA, have resulted in persistently elevated Hg concentrations in sediment, surface water, ground water, soil, and wildlife downstream of the discharge site. In the present study, we examined mercury (Hg) levels in in little brown bats (Myotis lucifugus) from this location and assessed the utility of a non-destructively collected tissue sample (wing punch) for determining mitochondrial DNA (mtDNA) damage in Hg exposed bats. Bats captured 1 and 3 km from the South River, exhibited significantly higher levels of total Hg (THg) in blood and fur than those from the reference location. We compared levels of mtDNA damage using real-time quantitative PCR (qPCR) analysis of two distinct regions of mtDNA. Genotoxicity is among the many known toxic effects of Hg, resulting from direct interactions with DNA or from oxidative damage. Because it lacks many of the protective protein structures and repair mechanisms associated with nuclear DNA, mtDNA is more sensitive to the effects of genotoxic chemicals and therefore may be a useful biomarker in chronically exposed organisms. Significantly higher levels of damage were observed in both regions of mtDNA in bats captured 3 km from the river than in controls. However, levels of mtDNA damage exhibited weak correlations with fur and blood THg levels, suggesting that other factors may play a role in the site-specific differences.

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“…As lead is nondegradable, its persistence in the environment can produce toxic effects to plants and animals. Once introduced into the body, Pb 2+ is a potential neurotoxin that can cause chronic inflammation of the kidney and heart, inhibit brain development, and decrease nerve conduction velocity [1][2][3]. The maximum level of lead in drinking water permitted by the US Environmental Protection Agency is 15 μg/L (∼72 nM) [4,5].…”

Section: Introductionmentioning

confidence: 99%

Colorimetric assay of lead using unmodified gold nanorods

et al. 2012

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Lead poisoning in adults can affect the peripheral and central nervous systems, the kidneys, and blood pressure. Thus, the development of environment-friendly and simple methods for Pb 2+ detection is of great importance. Herein, a label-free colorimetric method has been developed for the detection of Pb 2+ based on the conformational switch from single-stranded DNA to G-quadruplex. The electrostatic interactions between DNA probe and gold nanorods (GNRs) induce GNRs to space closely. However, the electrostatic interaction is not strong enough to change the suspension state of GNRs. In the presence of Pb 2+ , the formation of G-quadruplexes increases the surface charge density around DNA, which is expected to strengthen the electrostatic interaction between the GNRs and the DNA. Therefore, the longitudinal absorption of GNRs decreased because the stronger interaction induced aggregation of GNRs. Importantly, the decrease in longitudinal absorption is proportional to concentration of Pb 2+ . By monitoring the change of absorbance, Pb 2+ can be detected at a level of 3 nM with a linear range from 5 nM to 1 μM. The overall test only takes a few minutes and very little interference is observed from other metal ions. The major advantages of this method are its low cost, convenience, simplicity, sensitivity, and specificity.

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Heavy metals and DNA damage in blood cells of insectivore bats in coal mining areas of Catarinense coal basin, Brazil

Cited by 92 publications

References 60 publications

Chiroptera

Chiroptera

Assessment of mitochondrial DNA damage in little brown bats (Myotis lucifugus) collected near a mercury-contaminated river

Colorimetric assay of lead using unmodified gold nanorods

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