Heavy metal tolerance in marine strains of Yarrowia lipolytica

Abstract

Heavy metal tolerance of two marine strains of Yarrowia lipolytica was tested on solid yeast extract peptone dextrose agar plates. Based on minimum inhibitory concentration esteems, it is inferred that the two strains of Y. lipolytica were tolerant to heavy metals such as Pb(II), Cr(III), Zn(II), Cu(II), As(V), and Ni(II) ions. The impact of various heavy metal concentrations on the growth kinetics of Y. lipolytica was likewise assessed. With increased heavy metal concentration, the specific growth rate was reduced with delayed doubling time. Furthermore, biofilm development of both yeasts on the glass surfaces and in microtitre plates was assessed in presence of different heavy metals. In microtitre plates, a short lag phase of biofilm formation was noticed without the addition of heavy metals in yeast nitrogen base liquid media. A lag phase was extended over increasing metal concentrations of media. Heavy metals like Cr(VI), Cd(II), and As(V) are contrastingly influenced on biofilms' formation of microtitre plates. Other heavy metals did not much influence on biofilms development. Thus, biofilm formation is a strategy of Y. lipolytica under stress of heavy metals has significance in bioremediation process for recovery of heavy metals from contaminated environment.

Cadmium biosorption from wastewater by Yarrowia lipolytica AUMC 9256

“…J. Bot. 59, No.1 (2019) MaNal Tawfeek el-Sayed quantity of biomass has been produced (Bankar et al, 2012(Bankar et al, , 2018Bendigiri et al, 2018 andKatre et al, 2018). Moreover, it has inbuilt mechanisms for detoxifying heavy metals (Zinjarde et al, 2014).…”

Candida pseudoglaebosa and Kodamaea ohmeri are capable of degrading alkanes in the presence of heavy metals

“…Whereas neutral conditions (pH 7) made the strains extremely sensitive to HMs, acidic conditions (pH 3 and pH 5) permitted a greater tolerance to Cu 2+ , Zn 2+ , Pb 2+ , and As 3+ . Indeed, the relative tolerance level of the strains to each HM resembled that of Y. lipolytica and Cryptococcus vishniacii, for which Zn 2+ , Pb 2+ , and As 3+ are less toxic than Cd 2+ or Cu 2+ [46,47].…”

Heavy Metal Tolerance of Novel Papiliotrema Yeast Isolated from Vietnamese Mangosteen

“…Yeast cells are adaptable to environmental metal contamination and can withstand and detoxify such metals [7,8]. This is achieved through inherent mechanisms such as transformation, crystallization, complexation, extracellular precipitation, and cell wall adsorption [9].…”