Heavy metal tolerance in contrasting ecotypes of Alyssum montanum

Muszyńska, Ewa; Labudda, Mateusz; Różańska, Elżbieta; Hanus-Fajerska, Ewa; Znojek, Ewa

doi:10.1016/j.ecoenv.2018.05.075

Cited by 41 publications

(44 citation statements)

References 59 publications

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“…The activity of the other three antioxidant enzymescatalase (EC 1.11.1.6, CAT), guaiacol-type peroxidase (EC 1.11.1.7, GOPX) and glutathione peroxidase (EC 1.11.1.9, GPX)-was measured as described previously by Muszyńska et al (2018a) with minor modifications relying on the appropriate selection of the volume of enzyme extract used for the measurements.…”

Section: Measurements Of Antioxidant Enzyme Activitymentioning

confidence: 99%

“…The stressful environmental conditions stimulate the formation of reactive oxygen species (ROS) and their inactivation is controlled by various plant enzymatic and non-enzymatic mechanisms to prevent oxidation damage. Many experiments have highlighted the potential role of phenolic metabolites in ROS defense (Maestri et al 2010;Labudda et al 2016;Muszyńska et al 2018a;Piwowarczyk et al 2018). Phenols protect not only from oxidative stress by direct ROS scavenging or modification of lipid membrane fluidity, but might also chelate metal ions and prevent Fenton's reaction.…”

Section: Antioxidant Response Of Tested Ecotypesmentioning

confidence: 99%

“…Plant species or specialized ecotypes have adapted to excess amounts of HMs and exhibit a greater ability to survive in contaminated habitats than species from unpolluted sites (Mohtadi et al 2012;Muszyńska et al 2018a). One of them is Silene vulgaris which in Poland can be often found on meadows, fields and in forests (Koszelnik-Leszek and Bielecki 2013).…”

Section: Introductionmentioning

confidence: 99%

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Structural, physiological and genetic diversification of Silene vulgaris ecotypes from heavy metal-contaminated areas and their synchronous in vitro cultivation

Muszyńska

Labudda

Różańska

et al. 2019

Planta

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Main conclusion Results provide significant comparison of leaf anatomy, pigment content, antioxidant response and phenolic profile between individuals from miscellaneous populations and describe unified cultivation protocols for further research on stress biology.The plant communities growing on heavy metal-polluted areas have attracted considerable attention due to their unique ability to tolerate enormous amounts of toxic ions. Three ecotypes of Silene vulgaris representing calamine (CAL), serpentine (SER) and non-metallicolous (NM) populations were evaluated to reveal specific adaptation traits to harsh environment. CAL leaves presented a distinct anatomical pattern compared to leaves of SER and NM plants, pointing to their xeromorphic adaptation. These differences were accompanied by divergent accumulation and composition of photosynthetic pigments as well as antioxidant enzyme activity. In CAL ecotype, the mechanism of reactive oxygen species scavenging is based on the joint action of superoxide dismutase and catalase, but in SER ecotype on superoxide dismutase and guaiacol-type peroxidase. On the contrary, the concentration of phenylpropanoids and flavonols in the ecotypes was unchanged, implying the existence of similar pathways of their synthesis/degradation functioning in CAL and SER populations. The tested specimens showed genetic variation (atpA/MspI marker). Based on diversification of S. vulgaris populations, we focused on the elaboration of similar in vitro conditions for synchronous cultivation of various ecotypes. The most balanced shoot culture growth was obtained on MS medium containing 0.1 mg l −1 NAA and 0.25 mg l −1 BA, while the most abundant callogenesis was observed on MS medium enriched with 0.5 mg l −1 NAA and 5.0 mg l −1 BA. For the first time, unified in vitro protocols were described for metallophytes providing the opportunity to conduct basic and applied research on stress biology and tolerance mechanisms under freely controlled conditions.Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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Section: Measurements Of Antioxidant Enzyme Activitymentioning

confidence: 99%

Section: Antioxidant Response Of Tested Ecotypesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Structural, physiological and genetic diversification of Silene vulgaris ecotypes from heavy metal-contaminated areas and their synchronous in vitro cultivation

Muszyńska

Labudda

Różańska

et al. 2019

Planta

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“…To render the pauperization of plant cell functions impossible, the ROS content in cells is mastered by enzymatic and non‐enzymatic antioxidant mechanisms. The enzymatic machinery consists of superoxide dismutase (SOD), catalase (CAT), multifarious peroxidases and enzymes of the Foyer–Halliwell–Asada cycle (Labudda and Azam 2014 ; Muszyńska et al 2018 ; Kapoor et al 2019 ). The non‐enzymatic mechanisms are mainly based on the reduced glutathione (GSH) and ascorbate (ASA) and an abundant group of the phenol metabolites (Saxena et al 2016 ; Durak et al 2019 ; Muszyńska et al 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Reactive oxygen species metabolism and photosynthetic performance in leaves of Hordeum vulgare plants co-infested with Heterodera filipjevi and Aceria tosichella

Labudda

Tokarz

et al. 2020

Plant Cell Rep

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Key message Defence responses of cyst nematode and/or wheat curl mite infested barley engage the altered reactive oxygen species production, antioxidant machinery, carbon dioxide assimilation and photosynthesis efficiency. Abstract The primary aim of this study was to determine how barley responds to two pests infesting separately or at once; thus barley was inoculated with Heterodera filipjevi (Madzhidov) Stelter (cereal cyst nematode; CCN) and Aceria tosichella Keifer (wheat curl mite; WCM). To verify hypothesis about the involvement of redox metabolism and photosynthesis in barley defence responses, biochemical, photosynthesis efficiency and chlorophyll a fluorescence measurements as well as transmission electron microscopy were implemented. Inoculation with WCM (apart from or with CCN) brought about a significant suppression in the efficiency of electron transport outside photosystem II reaction centres. This limitation was an effect of diminished pool of rapidly reducing plastoquinone and decreased total electron carriers. Infestation with WCM (apart from or with CCN) also significantly restricted the electron transport on the photosystem I acceptor side, therefore produced reactive oxygen species oxidized lipids in cells of WCM and double infested plants and proteins in cells of WCM-infested plants. The level of hydrogen peroxide was significantly decreased in double infested plants because of glutathione–ascorbate cycle involvement. The inhibition of nitrosoglutathione reductase promoted the accumulation of S-nitrosoglutathione increasing antioxidant capacity in cells of double infested plants. Moreover, enhanced arginase activity in WCM-infested plants could stimulate synthesis of polyamines participating in plant antioxidant response. Infestation with WCM (apart from or with CCN) significantly reduced the efficiency of carbon dioxide assimilation by barley leaves, whereas infection only with CCN expanded photosynthesis efficiency. These were accompanied with the ultrastructural changes in chloroplasts during CCN and WCM infestation.

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“…The detailed monitoring of total petroleum hydrocarbon (TPH) content in the experimental soil was conducted every month over two years from the moment of soil pollution [27]. The analysis showed that after 24 months from the moment of soil contamination, TPH content in the soil contaminated with EO was more than 11 times higher than in the control soil, while in the soil contaminated with DF it was more than twice as high (16,530 mg kg −1 in EO, 3852 mg kg −1 in DF and 1458 mg kg −1 in control). The content of TPH in the soil contaminated with petrol (2661 mg kg −1 ) was similar to the control.…”

Section: Introductionmentioning

confidence: 99%

Soil Pollution by Petroleum-Derived Substances and its Bioremediation: The Effect on Aphis fabae Scop. Infestation and Antioxidant Response in Vicia faba L.

2020

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In this study, the effects of soil contamination with petroleum-derived substances (PDSs) (petrol, diesel fuel and used engine oil) and its bioremediation using biopreparation ZB-01 on broad bean infestation by black bean aphid Aphis fabae Scop., as well as on the antioxidant enzymes activity (superoxide dismutase (SOD), catalase (CAT) and guaiacol peroxidase (POD)) and antioxidant (non-protein thiols and proline) content in plant leaves, were determined. Results showed that after three years from the moment of soil contamination PDSs limited infestation of broad bean by A. fabae. However, the adverse effects on aphids’ life cycles were not proven. The lowered infestation may result from the lower attractiveness of contaminated plants to pests. PDSs significantly affected the activities of enzymes and the antioxidants content, with that effect being diversified. The increased activity of SOD was found in plants exposed to diesel fuel, together with the lowest numbers of aphids accompanying it, which can suggest a certain role of the enzyme in pest response to the stress caused by this PDS. The ZB-01 biopreparation limited the adverse effect of PDSs on the degree of broad bean plant infestation by A. fabae. Its influence on the antioxidant response was diversified. In the plants exposed to EO, changes in antioxidant response were reduced under the influence of ZB-01.

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Heavy metal tolerance in contrasting ecotypes of Alyssum montanum

Cited by 41 publications

References 59 publications

Structural, physiological and genetic diversification of Silene vulgaris ecotypes from heavy metal-contaminated areas and their synchronous in vitro cultivation

Structural, physiological and genetic diversification of Silene vulgaris ecotypes from heavy metal-contaminated areas and their synchronous in vitro cultivation

Reactive oxygen species metabolism and photosynthetic performance in leaves of Hordeum vulgare plants co-infested with Heterodera filipjevi and Aceria tosichella

Soil Pollution by Petroleum-Derived Substances and its Bioremediation: The Effect on Aphis fabae Scop. Infestation and Antioxidant Response in Vicia faba L.

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