Heavy metal-regulated new microRNAs from rice

Huang, Shudong; Peng, Jie; Qiu, Cheng; Yang, Zhi Min

doi:10.1016/j.jinorgbio.2008.10.019

Cited by 145 publications

(95 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous computational prediction and cloning studies reported 417 rice miRNA loci that produce 274 distinct mature miRNAs, as registered in miRBase release 14.0 Wang et al, 2004;Arazi et al, 2005;Liu et al, 2005;Sunkar et al, 2005Sunkar et al, , 2008Luo et al, 2006;Lu et al, 2008a;Morin et al, 2008;Zhu et al, 2008;Huang et al, 2009a;Johnson et al, 2009;Xue et al, 2009). After removing 11 double-registered miRNA genes in the same loci, 406 miRNA loci were analyzed.…”

Section: Evaluation Of Annotated Mirnasmentioning

confidence: 99%

Massive Analysis of Rice Small RNAs: Mechanistic Implications of Regulated MicroRNAs and Variants for Differential Target RNA Cleavage

et al. 2011

View full text Add to dashboard Cite

Small RNAs have a variety of important roles in plant development, stress responses, and other processes. They exert their influence by guiding mRNA cleavage, translational repression, and chromatin modification. To identify previously unknown rice (Oryza sativa) microRNAs (miRNAs) and those regulated by environmental stress, 62 small RNA libraries were constructed from rice plants and used for deep sequencing with Illumina technology. The libraries represent several tissues from control plants and plants subjected to different environmental stress treatments. More than 94 million genomematched reads were obtained, resulting in more than 16 million distinct small RNA sequences. This allowed an evaluation of ;400 annotated miRNAs with current criteria and the finding that among these, ;150 had small interfering RNA-like characteristics. Seventy-six new miRNAs were found, and miRNAs regulated in response to water stress, nutrient stress, or temperature stress were identified. Among the new examples of miRNA regulation were members of the same miRNA family that were differentially regulated in different organs and had distinct sequences Some of these distinct family members result in differential target cleavage and provide new insight about how an agriculturally important rice phenotype could be regulated in the panicle. This high-resolution analysis of rice miRNAs should be relevant to plant miRNAs in general, particularly in the Poaceae.

show abstract

Section: Evaluation Of Annotated Mirnasmentioning

confidence: 99%

Massive Analysis of Rice Small RNAs: Mechanistic Implications of Regulated MicroRNAs and Variants for Differential Target RNA Cleavage

et al. 2011

View full text Add to dashboard Cite

show abstract

“…miRNAs are short sequences (*21 nt) of endogenous non-coding RNA that negatively regulate gene expression at the post-transcriptional level [6,7]. miRNAs have been shown to play an important role in a variety of plant biological and metabolic processes including organ maturation [8][9][10][11][12], hormone signaling [13,14], developmental timing [15,16], response to pathogens [17][18][19], and response to environmental abiotic stresses such as drought [20], salinity [21], heavy metals [22], and cold [23].…”

Section: Introductionmentioning

confidence: 99%

Salt and Drought Stresses Induce the Aberrant Expression of microRNA Genes in Tobacco

et al. 2011

View full text Add to dashboard Cite

Drought and salinity stresses significantly altered microRNA (miRNA) expression in a dose-dependent manner in tobacco. Salinity stress changed the miRNA expression levels from a 6.86-fold down-regulation to a 616.57-fold up-regulation. Alternatively, miRNAs were down-regulated by 2.68-fold and up-regulated 2810-fold under drought conditions. miR395 was most sensitive to both stresses and was up-regulated by 616 and 2810-folds by 1.00% PEG and 0.171 M NaCl, respectively. Salinity and drought stresses also changed the expression of protein-coding genes [alcohol dehydrogenase (ADH) and alcohol peroxidase (APX)]. The results suggest that miRNAs may play an important role in plant response to environmental abiotic stresses. Further investigation of miRNA-mediated gene regulation may elucidate the molecular mechanism of plant tolerance to abiotic stresses and has the potential to create a miRNA-based biotechnology for improving plant tolerance to drought and salinity stresses.

show abstract

“…However, plants have multiple strategies to acclimate to heavy metal-induced toxicity. These include metal immobilization, exclusion, chelation, and gene regulation (Mehta and Gaur 1999;Hu et al 2001;Chen et al 2009b;Huang et al 2009). At the physiological level, establishment of an anti-oxidative system is one of the important strategies for plant adaptation to heavy metal stress.…”

Section: Introductionmentioning

confidence: 99%

“…Recent efforts have been directed to the global analysis of Curesponsive genes in plants (Sudo et al 2008). Very recently, Cd has been identified to regulate a set of microRNAs in rice and other plant species (Huang et al 2009(Huang et al , 2010. In contrast to the other heavy metals whose regulatory mechanisms have been well described, the process of Hg-induced toxicology in plants, particularly in green algae, is largely unknown.…”

Section: Introductionmentioning

confidence: 99%

Mercury-induced oxidative stress and impact on antioxidant enzymes in Chlamydomonas reinhardtii

et al. 2010

Self Cite

View full text Add to dashboard Cite

Investigation of mercury toxicology in green algae is of great importance from ecological point of view, because mercury has become a major contaminant in recent years. In higher plants, accumulation of mercury modifies many aspects of cellular functions. However, the process that mercury exerts detrimental effects on green algae is largely unknown. In this study, we performed an experiment focusing on the biological responses of Chlamydomonas reinhardtii, a unicellular model organism, to Hg(2+)-induced toxicity. C. reinhardtii was exposed to 0, 1, 2, 4, 6, and 8 μM Hg in media. Concentrations of Hg were negatively correlated with the cell growth. Treatment with Hg induced accumulation of reactive oxygen species and peroxidative products. Endogenous proline levels increased in Hg-exposed algae. Hg exposure activated superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX). To get insights into the molecular response, a RT-PCR-based assay was performed to analyze the transcript abundance of Mn-SOD, CAT and APX. Our analysis revealed that expression of the genes was up-regulated by Hg exposure, with a pattern similar to the enzyme activities. Additional investigation was undertaken on the effect of Hg on the transcript amount of ∆(1)-pyrroline-5-carboxylate synthetase, a key enzyme of proline biosynthesis and on that of heme oxygenase-1 (HO-1), an enzyme regulating heavy metal tolerance. Expressions of both P5CS and HO-1 were up-regulated by Hg. These data indicate that Hg-induced oxidative stress was responsible for the disturbance of the growth and antioxidant defensive systems in C. reinhardtii.

show abstract

Heavy metal-regulated new microRNAs from rice

Cited by 145 publications

References 51 publications

Massive Analysis of Rice Small RNAs: Mechanistic Implications of Regulated MicroRNAs and Variants for Differential Target RNA Cleavage

Massive Analysis of Rice Small RNAs: Mechanistic Implications of Regulated MicroRNAs and Variants for Differential Target RNA Cleavage

Salt and Drought Stresses Induce the Aberrant Expression of microRNA Genes in Tobacco

Mercury-induced oxidative stress and impact on antioxidant enzymes in Chlamydomonas reinhardtii

Contact Info

Product

Resources

About