Heavy metal inhibition of EROD activity in liver microsomes from the bass Dicentrarchus labrax exposed to organic xenobiotics: Role of GSH in the reduction of heavy metal effects

Viarengo, Aldo; Bettella, Elisa; Fabbri, Rita; Burlando, Bruno; Lafaurie, M.

doi:10.1016/s0141-1136(96)00097-9

Cited by 109 publications

(48 citation statements)

References 16 publications

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“…Sea bass liver EROD activity induction by B(a)P is in agreement with previous studies performed either in vivo with sea bass (Viarengo et al, 1997), eels (Pacheco and Santos, 1997b), carp (van der Weiden et al, 1994, turbot (Peters et al, 1997), #ounder (Eggens et al, 1996), and charr (Wolkers et al, 1996) or in vitro with rainbow trout liver cell line (Bols et al, 1999) and primary liver cell culture (Scholz and Segner, 1999). P450 increase induced by B(a)P was also reported in carp (van der Weiden et al, 1994) and #ounder (Eggens et al, 1996).…”

Section: Discussionsupporting

confidence: 93%

“…Some of those studies, which include mature (Novi et al, 1998) and immature (Lemaire et al, 1996;Viarengo et al, 1997;Jacksic et al, 1998) sea bass treated with di!erent BNF or 3-methylcholanthrene (3-MC) intraperitoneal injections, indicated EROD activity induction. Other sea bass studies revealed di!erences in liver and intestine B(a)P ultrastructural e!ects, as well as metabolic kinetics and metabolite distribution in relation to the route of exposure (Lemaire et al, 1990(Lemaire et al, , 1992.…”

Section: Introductionmentioning

confidence: 99%

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Juvenile Sea Bass Liver P450, EROD Induction, and Erythrocytic Genotoxic Responses to PAH and PAH-like Compounds

Gravato

Santos

2002

Ecotoxicology and Environmental Safety

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Juvenile Sea Bass Liver P450, EROD Induction, and Erythrocytic Genotoxic Responses to PAH and PAH-like Compounds

Gravato

Santos

2002

Ecotoxicology and Environmental Safety

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“…Some studies documented the accumulation of cadmium and copper in the gills, kidneys and liver and the pathological changes of varying severity in the above mentioned organs in sea bass. (Cattani et al, 1996;Giari et al, 2007;Romeo et al, 2000;Viarengo et al, 1997). In this work, we show preliminarily that the cultures of isolated blood leukocytes of D. labrax were able to incorporate Cd and Cu after treatment, thus confirming that these metals could cross membranes (for a review see Foulkes (2000)), and accumulate in the cells.…”

Section: Discussionsupporting

confidence: 66%

In vitro effect of cadmium and copper on separated blood leukocytes of Dicentrarchus labrax

Vazzana¹,

Celi²,

Tramati³

et al. 2014

Ecotoxicology and Environmental Safety

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a b s t r a c tThe immunotoxic effects of heavy metals on blood leukocytes of sea bass (Dicentrarchus labrax) were examined. The cells, separated by a discontinuous Percoll-gradients, were exposed in vitro to various sublethal concentrations of cadmium and copper (10 À 7 M, 10 À 5 M, and 10 À 3 M) and their immunotoxic effect was then evaluated by measuring neutral red uptake, MTT assay, DNA fragmentation and Hsp70 gene expression. First of all, we demonstrated that the cells treated in vitro could incorporate Cd and Cu. A relationship between heavy metal exposure and dose-time-dependent alterations in responses of leukocytes from blood was found for both metals, but copper was more immunotoxic than cadmium in all assays performed. A significant reduction in the cells' ability to uptake neutral red and viability by MTT assay was recorded, indicating that both cadmium and copper could change the membrane permeability, inducing cellular apoptosis when the concentration of metals reached 10 À 3 M. The apoptotic effect may also explain the high level of cytotoxicity found when the leukocytes were exposed to higher concentration of metals. These results demonstrated that toxic effect of copper and cadmium affect on the mechanisms of cellmediated immunity reducing the immune defences of the organism.

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“…Positive correlations were also found for EROD activity and all metals in sediment (the correlation coefficients ranged from 0.51 to 0.86), but only for Cr (r ¼ 0.86) and Mo (r ¼ 0.79) were they statistically significant ( p < 0.05). Although metals are known to inhibit many enzymatic activities in aquatic organisms [37,38], the present study obtained different results. These nonstandard results can be caused by the presence of other pollutants (e.g., PCB or PAH), which are known as Ah-receptor agonists, and could be involved in EROD activity increasing.…”

Section: Enzymes Of Phase I Of Xenobiotics Detoxificationmentioning

confidence: 54%

Assessment of contamination of the Svitava and Svratka rivers in the Czech Republic using selected biochemical markers

Blahová

Havelková

Kružíková

et al. 2010

Enviro Toxic and Chemistry

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The aim of the present study is to assess aquatic ecosystem contamination using selected biochemical markers: cytochrome P450, ethoxyresorufin-O-deethylase (EROD), glutathione S-transferase (GST), tripeptide glutathione, vitellogenin, and 11-ketotestosterone in chub (Leuciscus cephalus L.). Seven locations on the Svitava and Svratka rivers (in the Brno conurbation, Czech Republic) were assessed. The results were compared with the levels of the most important inductors of these biomarkers: organic pollutants hexachlorocyclohexane (HCH), hexachlorobenzene (HCB), DDT and its metabolites, polychlorinated biphenyls, and polycyclic aromatic hydrocarbons in bottom sediment, fish muscle, and semipermeable membrane devices (SPMDs) and metals in sediment. The highest levels of pollutants were observed at sites situated downstream from Brno, especially at Modrice and Rajhradice. Significant positive correlations (p < 0.05) were found between EROD activity and HCH concentration in SPMDs, and also between GST and EROD activity with HCB concentration in muscle, after adjusting for age.

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Heavy metal inhibition of EROD activity in liver microsomes from the bass Dicentrarchus labrax exposed to organic xenobiotics: Role of GSH in the reduction of heavy metal effects

Cited by 109 publications

References 16 publications

Juvenile Sea Bass Liver P450, EROD Induction, and Erythrocytic Genotoxic Responses to PAH and PAH-like Compounds

Juvenile Sea Bass Liver P450, EROD Induction, and Erythrocytic Genotoxic Responses to PAH and PAH-like Compounds

In vitro effect of cadmium and copper on separated blood leukocytes of Dicentrarchus labrax

Assessment of contamination of the Svitava and Svratka rivers in the Czech Republic using selected biochemical markers

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