Heavy ion induced damage to plasmid DNA: plateau region vs. spread out Bragg-peak

Dang, H. M.; Goethem, M. J. van; Graaf, E.R. van der; Brandenburg, S.; Hoekstra, Ronnie; Schlathölter, Thomas

doi:10.1140/epjd/e2011-10679-1

Cited by 20 publications

(33 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results from our study using carbon beam are in good agreement with findings of Dang et al (2011), who used the same plasmid solute in mannitol of different concentrations (corresponding scavenging capacities within the range from 1.8 9 10 5 to 1.1 9 10 9 M -1 s -1 ), and the source was a carbon beam of slightly higher LET. On the other hand, Terato et al (2008) found one order of magnitude less DSB in plasmid pDEL19 (4814 bp) and lambda DNA (48502 bp) solute in TE (scavenging capacity 1.5 9 10 7 M -1 s -1 ) irradiated by the same carbon and iron beams at HIMAC facility, while the amounts of SSB were in good agreement with our values.…”

Section: Strand Breakssupporting

confidence: 90%

“…Only few data of strand breaks induced by comparable beams are available (Terato et al 2008;Dang et al 2011). The results from our study using carbon beam are in good agreement with findings of Dang et al (2011), who used the same plasmid solute in mannitol of different concentrations (corresponding scavenging capacities within the range from 1.8 9 10 5 to 1.1 9 10 9 M -1 s -1 ), and the source was a carbon beam of slightly higher LET.…”

Section: Strand Breakssupporting

confidence: 89%

“…On the other hand, Terato et al (2008) found one order of magnitude less DSB in plasmid pDEL19 (4814 bp) and lambda DNA (48502 bp) solute in TE (scavenging capacity 1.5 9 10 7 M -1 s -1 ) irradiated by the same carbon and iron beams at HIMAC facility, while the amounts of SSB were in good agreement with our values. Dang et al (2011) calculated the SSB and DSB yields with a model based on the work used in our study (Cowan et al 1987);Terato et al (2008) used different model, described in (Milligan et al 2000). To investigate the difference between models, we tested them on our data.…”

Section: Strand Breaksmentioning

confidence: 99%

See 2 more Smart Citations

Clustered DNA damage on subcellular level: effect of scavengers

Brabcová

Sihver

Yasuda

et al. 2014

Radiat Environ Biophys

View full text Add to dashboard Cite

Clustered DNA damages are induced by ionizing radiation, particularly of high linear energy transfer (LET). Compared to isolated DNA damage sites, their biological effects can be more severe. We investigated a clustered DNA damage induced by high LET radiation (C 290 MeV u(-1) and Fe 500 MeV u(-1)) in pBR322 plasmid DNA. The plasmid is dissolved in pure water or in aqueous solution of one of the three scavengers (coumarin-3-carboxylic acid, dimethylsulfoxide, and glycylglycine). The yield of double strand breaks (DSB) induced in the DNA plasmid-scavenger system by heavy ion radiation was found to decrease with increasing scavenging capacity due to reaction with hydroxyl radical, linearly with high correlation coefficients. The yield of non-DSB clusters was found to occur twice as much as the DSB. Their decrease with increasing scavenging capacity had lower linear correlation coefficients. This indicates that the yield of non-DSB clusters depends on more factors, which are likely connected to the chemical properties of individual scavengers.

show abstract

Section: Strand Breakssupporting

confidence: 90%

Section: Strand Breakssupporting

confidence: 89%

Section: Strand Breaksmentioning

confidence: 99%

See 1 more Smart Citation

Clustered DNA damage on subcellular level: effect of scavengers

Brabcová

Sihver

Yasuda

et al. 2014

Radiat Environ Biophys

View full text Add to dashboard Cite

show abstract

“…where θ is the Heaviside function and R = 10 nm × S e /S e,CBP with S e,CBP being the LET of carbon ions at their Bragg peak. The value of N r was estimated as 0.08 from the comparison of the experimental results [39] for plasmid DNA dissolved in pure water and in a scavenger-rich solution. Again, more work is needed to obtain more detailed dependencies of N r and R on LET.…”

Section: The Msa Methodologymentioning

confidence: 99%

Phenomenon-based evaluation of relative biological effectiveness of ion beams by means of the multiscale approach

2019

View full text Add to dashboard Cite

Relative biological effectiveness (RBE) is a key quantity for the description of radiobiological effects induced by charged-particle irradiation in the context of ion-beam cancer therapy. Since RBE is a complex function that depends on different physical, chemical and biological parameters, a fundamental understanding of these effects becomes increasingly important for clinical applications.The phenomenon-based MultiScale Approach to the physics of radiation damage with ions (MSA) provides a tool for a molecular-level understanding of physical and chemical mechanisms of radiation biodamage and allows to quantify macroscopic biological effects caused by ion irradiation. This study reports the first application of the MSA for the analysis of RBE of ion beams. As a case study, we quantify the response of human normal tissue cell lines to carbon-ion irradiation at different values of linear energy transfer. RBE corresponding to different biological endpoints as well as other radiobiological parameters, such as clonogenic cell survival as a function of dose and inactivation cross section, are analyzed and compared with experimental data. Good agreement with an extensive data set emphasizes predictive power of the MSA. The method is also used to make predictions of RBE at high values of LET where RBE decreases due to the "overkill" effect. In this regime, the dose needed to achieve a given biological effect is deposited by a few ions. The fact that a given number of ions may produce more damage than needed for a given effect leads to a significant (up to 20%) variation in RBE. This effect should be taken into consideration in the analysis of experimental data on irradiation with high-LET ions.Keywords: ion-beam cancer therapy; multiscale approach; relative biological effectiveness; cell survival; overkill effect Phenomenon-based prediction of RBE of ion beams by means of the MSA

show abstract

“…where is the Heaviside function and R(LET) = 10 nm × LET/LET CBP , with LET CBP being the LET of carbon ions in the Bragg peak, and where R CBP ≃ 10 nm corresponds to a conservative estimate for the Bragg peak of carbon (Surdutovich and Solov'yov 2014). N r has been estimated to be 0.08 in Verkhovtsev et al (2016), based on experimental information (Dang et al 2011). Still, more work is needed to derive a more precise relation for N r (ρ) from molecular dynamics simulations.…”

Section: Impact Of Shock Waves On the Radiation Chemistry And Indirecmentioning

confidence: 99%