Heating-induced contraction in airways smooth muscle: A possible causative factor of exercise-induced bronchoconstriction

Mustafa, Seham

doi:10.1016/j.resp.2018.11.001

Cited by 4 publications

(2 citation statements)

References 36 publications

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“…Pentobarbital sodium (50 mg/kg) was administered intraperitoneally to anesthetize the animals before exsanguination. Tracheas were removed and placed in a plate containing modified Krebs–Henseleit solution with the following composition (in mM): 118 NaCl, 25 NaHCO 3 , 4.6 KCl, 1.2 KH 2 PO 4 , 1.2 MgSO 4 , 2 CaCl 2 , 11 glucose [ 30 , 34 , 90 , 91 , 92 , 93 , 94 ]. Tissues were dissected and freed from connective tissue and epithelium.…”

Section: Methodsmentioning

confidence: 99%

TRPV4 Activation during Guinea Pig Airway Smooth Muscle Contraction Promotes Ca2+ and Na+ Influx

Montaño,

Carbajal-García,

Casas-Hernández

et al. 2024

Pharmaceuticals

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Airway smooth muscle (ASM) contraction is determined by the increase in intracellular Ca2+ concentration ([Ca2+]i) caused by its release from the sarcoplasmic reticulum (SR) or by extracellular Ca2+ influx. Major channels involved in Ca2+ influx in ASM cells are L-type voltage-dependent Ca2+ channels (L-VDCCs) and nonselective cation channels (NSCCs). Transient receptor potential vanilloid 4 (TRPV4) is an NSCC recently studied in ASM. Mechanical stimuli, such as contraction, can activate TRPV4. We investigated the possible activation of TRPV4 by histamine (His)- or carbachol (CCh)-induced contraction in guinea pig ASM. In single myocytes, the TRPV4 agonist (GSK101) evoked an increase in [Ca2+]i, characterized by a slow onset and a plateau phase. The TRPV4 antagonist (GSK219) decreased channel activity by 94%, whereas the Ca2+-free medium abolished the Ca2+ response induced by GSK101. Moreover, GSK101 caused Na+ influx in tracheal myocytes. GSK219 reduced the Ca2+ peak and the Ca2+ plateau triggered by His or CCh. TRPV4 blockade shifted the concentration–response curve relating to His and CCh to the right in tracheal rings and reduced the maximal contraction. Finally, the activation of TRPV4 in single myocytes increased the Ca2+ refilling of the SR. We conclude that contraction of ASM cells after stimulation with His or CCh promotes TRPV4 activation, the subsequent influx of Ca2+ and Na+, and the opening of L-VDCCs. The entry of Ca2+ into ASM cells via TRPV4 and L-VDCCs contributes to optimal smooth muscle contraction.

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Section: Methodsmentioning

confidence: 99%

TRPV4 Activation during Guinea Pig Airway Smooth Muscle Contraction Promotes Ca2+ and Na+ Influx

Montaño,

Carbajal-García,

Casas-Hernández

et al. 2024

Pharmaceuticals

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“…Third, there is evidence that body temperature influences contractility of bronchiolar smooth muscle, whereby deviations of normal body temperature evoke contraction of the airway smooth muscle. This might be causative in bronchoconstriction (Mustafa, 2019). Therefore, airway obstruction, second to vagally-mediated bronchoconstriction and/or pro-inflammatory airway hyperresponsiveness, may underpin a change in lung function associated with cold-water ingestion.…”

Section: Temperature Effectsmentioning

confidence: 99%