Heated Scallop-Shell Powder Treatment for Deactivation and Removal of &lt;i&gt;Listeria&lt;/i&gt; sp. Biofilm Formed at a Low Temperature

Kefir is a microbial complex capable of producing biofilms. This study aims to obtain biofilms associated with hydroethanolic extract of Euterpe oleracea Mart. (BEHEEo) as fixing method of recovery anthocyanins in kefir biofilm. Thus, BEHEEo formation kinetics was made at different concentrations of aqueous extract of E. oleracea, of kefir grains (KG) and of brown sugar (BS). Thereafter, macro and microelements of BEHEEo were determined; the rheological and structural characteristics were analyzed using atomic force microscopy (AFM), and anthocyanins dosage in BEHEEo was obtained. The best concentration of BS to form BEHEEo was 40 g/l with 100 ml of EHEEo (12.62±0.16 g), as well as a release of the BEHEEo anthocyanins occurring after 5 min (4.3±0.6 mg/100 g) with maximum peak at 60 min (20.8±0.3). The concentration of calcium iron and magnesium was 0.089, 0.1740 and 0.1808 mg/kg, respectively, indicating the concentration of Zn2+ which was 0.533 mg/kg. The AFM analysis revealed differences in the peaks of roughness depending on the concentration of kefir grains, with presence of Lactobacillus and yeast. The concentration of anthocyanins in BEHEEo two years after the incorporation was 26 mg/100 g. Therefore, it is suggested that the BEHEEo incorporating EHEEo anthocyanins has potential for therapeutic applications in several pathologies necessary for antioxidative processes.Key words: Biofilms, kefir, açaí, Euterpe oleracea Mart., anthocyanins, atomic force microscopy (AFM). INTRODUCTIONKefir is a beverage produced by fermenting substrates such as milk or sugar water by kefir grains (Rodrigues et al., 2005;Laureys and De Vuyst, 2014). The growth and the function of bacteria inside a population are crucial for their survival (Davey and O'toole, 2000). In kefir, there is a predominance of Lactobacillus which has high catabolic capacity and is complemented by yeast with high biosynthetic capacity.When growing kefir in water for more than twenty days, there is the formation of biofilms with therapeutic properties against pathogenic organisms. These biofilms are surfaces with a high rate of structural organization associated with bacterial colonies held together by a polymer secreted by their cells, which is called extracellular polymeric substance (EPS) (Watnick and Kolter, 2000, Donlan, 2002;Zhang et al., 2015). Among many strategies used for the study of biofilms, the one that is more highlighted is the use of the Atomic Force Microscopy (AFM), which allows measurement of the interaction forces between cells or cell-cell interactions (Dufrêne, 2015).One of the substances that have been intensified in recent years are the anthocyanins, due to their protective and healing properties. Anthocyanins have an important role in physiological functions related to human health (Lee et al., 2013). The antioxidant effect of anthocyanins have been known and proven fact. The action mechanism of the anthocyanin is inhibiting the free radicals that promote oxidation. The antioxidant agents capable of binding to the free radicals ...

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“…Biofilms formed by Listeria sp. are more resistant to temperature of 15°C than at 37°C (Shimamura et al, 2015). BEHEEo are not formed at 37°C.…”

Section: Discussionmentioning

confidence: 96%

Study of kefir biofilm associated with hydroethanolic extract of Euterpe oleracea Mart. (aai)

Oliveira¹,

Maciel²,

Florentino³

et al. 2017

Afr. J. Microbiol. Res.

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“…Separation of two layers due to flocculation/precipitation was observed in all samples, except under acidic conditions. The TC and CF cell counts in supernatants of the BiSCaO and BiSCa(OH) 2 colloidal dispersions at pH ≥ 11 were much lower compared with samples at other pH values (pH ≤ 10), and the antimicrobial effect was more pronounced with BiSCaO colloidal dispersions than with BiSCa(OH) 2 colloidal dispersions at each pH (pH [5][6][7][8][9][10][11][12]. No TC and CF were observed following treatment with BiSCaO colloidal dispersion at pH 12 ( Figure 5).…”

Section: Antimicrobial Efficacy Of Biscao and Bisca(oh) 2 Colloidal Dmentioning

confidence: 97%

“…The main component of scallop shells is calcium carbonate (CaCO 3 ), which is converted to CaO when heated at over 800 • C. Heated scallop shell powder is composed mainly of CaO, which we called bioshell calcium oxide (BiSCaO), a material well known to exhibit strong antimicrobial activity [4]. For example, BiSCaO shows broad antimicrobial action against the avian influenza virus [5], bacteria [3,4], heat-resistant bacterial spores [3,6], fungi [7], and biofilms [8][9][10]. In addition, BiSCaO is used as an additive to prolong the shelf life of food products [10].…”

Section: Introductionmentioning

confidence: 99%

“…For example, BiSCaO shows broad antimicrobial action against the avian influenza virus [5], bacteria [3,4], heat-resistant bacterial spores [3,6], fungi [7], and biofilms [8][9][10]. In addition, BiSCaO is used as an additive to prolong the shelf life of food products [10]. CaO is easily converted to Ca(OH) 2 by hydration with water and air moisture.…”

Section: Introductionmentioning

confidence: 99%

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Application of Colloidal Dispersions of Bioshell Calcium Oxide (BiSCaO) for Disinfection

et al. 2019

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Bioshell calcium oxide (BiSCaO) is a scallop-shell powder heated at a high temperature. BiSCaO is composed mainly of calcium oxide and exhibits broad microbicidal properties. The aim of this study is to evaluate the disinfection and decontamination abilities of BiSCaO colloidal dispersions with that of commercially available bioshell calcium hydroxide (BiSCa(OH)2) following the formation of flocculants/precipitates under strongly alkaline conditions (pH 11.5–12.2). Various concentrations of BiSCaO and BiSCa(OH)2 colloidal dispersions were prepared by mixing with Na-polyPO4 (PP) and Na-triPO4 (TP) as flocculating agents. The microbicidal activities, and the degree of flocculation/precipitation of trypan blue, albumin, chondroitin sulfate, heparin, non-anticoagulant heparin carrying polystyrene (NAC-HCPS), and low-molecular-weight heparin/protamine nanoparticles (LMWH/P NPs) were dependent on the pH, the average particle diameter, and the concentration of BiSCaO or BiSCa(OH)2 and of the phosphate compound. BiSCaO (average particle diameter: 6 μm) colloidal dispersions (0.2 wt.%) containing 0.15 wt.% PP or TP exhibited substantially stronger microbicidal activity and flocculation/precipitation under strongly alkaline conditions. These results suggest that BiSCaO colloidal dispersions together with phosphate compounds have practical applicability for disinfection.

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“…HSSP was reported to exhibit antimicrobial activity against bacteria Bae et al, 2006;Ro et al, 2015, 39 , fungi Sawai andShiga, 2006;Xing et al, 2013, heatresistant bacterial spores Sawai et al, 2003viruses Thammakarn et al, 2014; . In addition, HSPP treatment killed and eliminated bacterial biofilms as well as planktonic cells Bodur and Cagri-Mehmetogl, 2012;Kubo et al, 2013;Sawai et al, 2013;Shimamura et al, 2015 . Recently, nanoparticles of HSSP were also shown to have higher bactericidal, sporicidal, and virucidal effects than microparticles of HSSP Jeong et al, 2007;Watanabe et al, 2014 .…”

Section: Observation Of Caco 3 Scale Generationmentioning

confidence: 99%

Sorbitol Minimizes Calcium Carbonate Scale Generation While Maintaining the Disinfection Effect of Heated Scallop-Shell Powder for Fresh Produce

et al. 2018

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Scallop shells subjected to heat treatment exhibit antimicrobial activity, and heated scallopshell powder HSSP has recently been reported to be effective for disinfecting food. However, because the main component of these shells is calcium oxide, there is a problem that scales of calcium carbonate CaCO 3 become established on the surface of equipment used for food processing. In this study, we thus investigated whether the addition of sugar to HSSP slurry suppressed CaCO 3 scale generation and whether the sugar-supplemented HSSP could be applied to the disinfection and preservation of fresh lettuce. The results showed that glucose, sucrose, and sorbitol could suppress the scale generation in HSSP slurry. However, glucose and sucrose decreased the antibacterial activity of HSSP. Since the addition of sorbitol did not affect the antibacterial activity of HSSP slurry, it was used for subsequent experiments because of its low bioavailability. Sorbitol effectively suppressed scale formation by dissolving it before the addition of HSSP. The disinfection and preservative effects of sorbitol-supplemented HSSP S-HSSP treatment on lettuce did not decrease compared with those upon HSSP treatment and were almost equal to or higher than those of sodium hypochlorite treatment at 200 mg/l. The addition of sorbitol solved the major problem of scale generation by HSSP containing CaO, which contributes to expansion of usage of heated shell powder, such as HSSP, in food processing.

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Heated Scallop-Shell Powder Treatment for Deactivation and Removal of <i>Listeria</i> sp. Biofilm Formed at a Low Temperature

Cited by 22 publications

References 19 publications

Study of kefir biofilm associated with hydroethanolic extract of Euterpe oleracea Mart. (aai)

Study of kefir biofilm associated with hydroethanolic extract of Euterpe oleracea Mart. (aai)

Application of Colloidal Dispersions of Bioshell Calcium Oxide (BiSCaO) for Disinfection

Sorbitol Minimizes Calcium Carbonate Scale Generation While Maintaining the Disinfection Effect of Heated Scallop-Shell Powder for Fresh Produce

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