The power generation mainly depends on fossil diesel fuel, the primary source of harmful emissions and global warming. Therefore, the researcher aims to explore alternative fuels that got greater attention in compression ignition engines. The commercial Diesel-RK software simulates the current numerical study of diesel engine direct injection with speed engine 1500 rpm, compression ratio 17.5, single cylinder, and naturally aspirated. In the basis of the parliamentary research, the literature did not investigate the influence of combustion and emission characteristics of compression ignition engines by using various double and trouble injection strategies along with different injector nozzle bore sizes. Also, the initiative was undertaken to study the effect of the different diesel-biodiesel blends ratio studied, SP20 (80% diesel+20% spirulina), Sp40(60% diesel+40% spirulina), and Sp100(0% diesel +100% spirulina) While the scope of the gap expanded to include a comparison of results with baseline diesel fuel. The results show that MPR increased by 4.2%, maximum gas temperature increased by 8.9%, ignition delay increased by 7.9%, maximum heat release rate decreased by 9.5%, NOx decreased by 7.8%, CO2 decreased by 3.9%, and particularly matter emissions decreased by 6.3% were compared to the double injection scheme , at 0.2 mm(INB)