Heat exchanger has wide use in many application such as in food industries, chemical, transportation, and refrigeration and air conditioning. Fan coil unit (FCU), one class of heat exchanger which is heat transfer occur by chilled water flowing across a series of finned tube bank for decreasing the air temperature to be cooled. Thermal conductivity of working fluid in heat exchanger was the main issue recently. Enhancement of convective heat transfer and thermal conductivity of working fluid made possible by mixing nanosize particle into base fluid. The aims of this research is to revealed the enhanced of heat transfer by nanofluid Al2O3 on FCU unit of chiller system. 0,2% of nanoparticles were added to chilled water. The analysis shows that added small part of nanoparticles induced heat transfer. The total heat transfer enhancement of nanofluid chilled water was about 8.0% -11.1% compare to that of chilled water without nanoparticles. Finally, the application of nanoparticles give benefit of heat transfer process.