Heat Tolerance of Gammarus fossarum (Crustacea, Amphipoda) Is Influenced by the Level of Stress Associated with Reproduction and the Water Quality of Their Habitat

Abstract

To elucidate how rising temperatures influence native amphipods, and how the expansion of wastewater treatment plants (WWTPs) with a fourth purification stage can alter temperature-related effects, studies were conducted in anthropogenically influenced rivers in Southwest Germany. Gammarids were sampled up- and downstream of two WWTPs, and exposed to elevated temperatures in the laboratory. Gammarids separated from precopula pairs were more sensitive to exposure-related stress and to a temperature increase than animals that were not in the reproductive phase. Furthermore, females were significantly more sensitive than males. Adult gammarids sampled individually at the WWTP with three purification stages showed a similar tolerance to elevated temperature at the up- and downstream site. In contrast, mortality following heat stress was substantially lower in animals sampled downstream of the WWTP with four treatment stages than in those sampled at the upstream site. We assume that the increased sensitivity of artificially separated precopula pairings reflect the situation under high hydraulic stress after heavy rainfall. Our results further illustrate the importance of WWTP upgrades in the context of climate change: gammarids sampled downstream the upgraded fourth-stage WWTP were likely exposed to lower levels of micropollutants, and thus may have allocated energy from detoxification to heat stress responses.