Heat stress response mechanisms in pollen development

Chaturvedi, Palak; Wiese, Anna Johanna; Ghatak, Arindam; Drábková, Lenka Záveská; Weckwerth, Wolfram; Honys, David

doi:10.1111/nph.17380

Cited by 114 publications

(110 citation statements)

References 210 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Transcriptome profiling offers a global snapshot of the entire RNA molecules, including mRNA, tRNA, rRNA, sRNA, and other non-coding RNA within a cell, tissue, organ, or whole organism at any given time point, which is not possible to be investigated at the genomic level ( Weckwerth et al, 2020 ; Chaturvedi et al, 2021 ). Understanding the transcriptome is crucial for deducing the genome‘s functional elements and revealing the molecular components of cells or tissues, understanding cells‘ responses to developmental and environmental stimuli triggered changes ( Wang et al, 2009 ).…”

Section: Omics Facilitated Crop Improvement For Abiotic and Biotic Stress Resistancesmentioning

confidence: 99%

Omics-Facilitated Crop Improvement for Climate Resilience and Superior Nutritive Value

et al. 2021

View full text Add to dashboard Cite

Novel crop improvement approaches, including those that facilitate for the exploitation of crop wild relatives and underutilized species harboring the much-needed natural allelic variation are indispensable if we are to develop climate-smart crops with enhanced abiotic and biotic stress tolerance, higher nutritive value, and superior traits of agronomic importance. Top among these approaches are the “omics” technologies, including genomics, transcriptomics, proteomics, metabolomics, phenomics, and their integration, whose deployment has been vital in revealing several key genes, proteins and metabolic pathways underlying numerous traits of agronomic importance, and aiding marker-assisted breeding in major crop species. Here, citing several relevant examples, we appraise our understanding on the recent developments in omics technologies and how they are driving our quest to breed climate resilient crops. Large-scale genome resequencing, pan-genomes and genome-wide association studies are aiding the identification and analysis of species-level genome variations, whilst RNA-sequencing driven transcriptomics has provided unprecedented opportunities for conducting crop abiotic and biotic stress response studies. Meanwhile, single cell transcriptomics is slowly becoming an indispensable tool for decoding cell-specific stress responses, although several technical and experimental design challenges still need to be resolved. Additionally, the refinement of the conventional techniques and advent of modern, high-resolution proteomics technologies necessitated a gradual shift from the general descriptive studies of plant protein abundances to large scale analysis of protein-metabolite interactions. Especially, metabolomics is currently receiving special attention, owing to the role metabolites play as metabolic intermediates and close links to the phenotypic expression. Further, high throughput phenomics applications are driving the targeting of new research domains such as root system architecture analysis, and exploration of plant root-associated microbes for improved crop health and climate resilience. Overall, coupling these multi-omics technologies to modern plant breeding and genetic engineering methods ensures an all-encompassing approach to developing nutritionally-rich and climate-smart crops whose productivity can sustainably and sufficiently meet the current and future food, nutrition and energy demands.

show abstract

Section: Omics Facilitated Crop Improvement For Abiotic and Biotic Stress Resistancesmentioning

confidence: 99%

Omics-Facilitated Crop Improvement for Climate Resilience and Superior Nutritive Value

et al. 2021

View full text Add to dashboard Cite

show abstract

“…An outstanding question in the field is why pollen development and function are particularly sensitive to heat ( Rieu et al, 2017 ). Heat sensitivity at meiosis of the pollen mother cell, microspore formation, pollen development, and tapetum development within the anther was reviewed before ( De Storme and Geelen, 2014 ; Rieu et al, 2017 ; Chaturvedi et al, 2021 ). Here we primarily focus on pollen maturation and germination to reveal insights into pollen responses to developmental cues and environmental stress.…”

Section: Introductionmentioning

confidence: 99%

Holistic insights from pollen omics: co-opting stress-responsive genes and ER-mediated proteostasis for male fertility

et al. 2021

View full text Add to dashboard Cite

Sexual reproduction in flowering plants takes place without an aqueous environment. Sperm are carried by pollen through air to reach the female gametophyte, though the molecular basis underlying the protective strategy of the male gametophyte is poorly understood. Here we compared the published transcriptomes of Arabidopsis thaliana pollen, and of heat-responsive genes, and uncovered insights into how mature pollen tolerates desiccation, while developing and germinating pollen are vulnerable to heat stress. Germinating pollen expresses molecular chaperones or ‘heat shock proteins’ in the absence of heat stress. Furthermore, pollen tubes that grew through pistils at basal temperature showed induction of the ER stress response, which is a characteristic of stressed vegetative tissues. Recent studies show mature pollen contains mRNA–protein aggregates that resemble ‘stress’ granules triggered by heat or other stresses to protect cells. Based on these observations, we postulate that mRNA–protein particles are formed in maturing pollen in response to developmentally programmed dehydration. Dry pollen can withstand harsh conditions as it is dispersed in air. We propose that, when pollen lands on a compatible pistil and hydrates, mRNAs stored in particles are released, aided by molecular chaperones, to become translationally active. Pollen responds to osmotic, mechanical, oxidative and peptide cues that promote ER-mediated proteostasis and membrane trafficking for tube growth and sperm discharge. Unlike vegetative tissues, pollen depends on stress-protection strategies for its normal development and function. Thus, heat stress during reproduction likely triggers changes that interfere with the normal stress responses, thereby compromising male fertility. This holistic perspective provides a framework to understand the basis of heat-tolerant strains in the reproduction of crops.

show abstract

“…Particularly, research has been focused on plant heat stress (HS) tolerance mechanisms, as higher temperatures have a negative effect on plant growth and production [2,3]. Although plants are susceptible to HS throughout their lifespan, reproductive tissues are specifically characterized by vulnerability to HS [4,5]. Heatwaves are expected to become more frequent in the future, and atmospheric temperature is on course to rise ~4 • C by the end of this century [6,7].…”

Section: Introductionmentioning

confidence: 99%

In Silico Characterization and Expression Profiles of Heat Shock Transcription Factors (HSFs) in Maize (Zea mays L.)

et al. 2021

View full text Add to dashboard Cite

Heat shock transcription factors (HSFs) regulate many environmental stress responses and biological processes in plants. Maize (Zea mays L.) is a major cash crop that is grown worldwide. However, the growth and yield of maize are affected by several adverse environmental stresses. Therefore, investigating the factors that regulate maize growth and development and resistance to abiotic stress is an essential task for developing stress-resilient maize varieties. Thus, a comprehensive genome-wide identification analysis was performed to identify HSFs genes in the maize genome. The current study identified 25 ZmHSFs, randomly distributed throughout the maize genome. Phylogenetic analysis revealed that ZmHSFs are divided into three classes and 13 sub-classes. Gene structure and protein motif analysis supported the results obtained through the phylogenetic analysis. Segmental duplication is shown to be responsible for the expansion of ZmHSFs. Most of the ZmHSFs are localized inside the nucleus, and the ZmHSFs which belong to the same group show similar physio-chemical properties. Previously reported and publicly available RNA-seq analysis revealed a major role of class A HSFs including ZmHSFA-1a and ZmHSFA-2a in all the maize growth stages, i.e., seed, vegetative, and reproductive development. Under abiotic stress conditions (heat, drought, cold, UV, and salinity), members of class A and B ZmHSFs are induced. Gene ontology and protein–protein interaction analysis indicated a major role of ZmHSFs in resistance to environmental stress and regulation of primary metabolism. To summarize, this study provides novel insights for functional studies on the ZmHSFs in maize breeding programs.

show abstract

Heat stress response mechanisms in pollen development

Cited by 114 publications

References 210 publications

Omics-Facilitated Crop Improvement for Climate Resilience and Superior Nutritive Value

Omics-Facilitated Crop Improvement for Climate Resilience and Superior Nutritive Value

Holistic insights from pollen omics: co-opting stress-responsive genes and ER-mediated proteostasis for male fertility

In Silico Characterization and Expression Profiles of Heat Shock Transcription Factors (HSFs) in Maize (Zea mays L.)

Contact Info

Product

Resources

About