Heat Stress Reduces Sperm Motility via Activation of Glycogen Synthase Kinase-3α and Inhibition of Mitochondrial Protein Import

Gong, Yabin; Guo, Huijuan; Zhang, Zhilong; Zhou, Hao; Zhao, Ruqian

doi:10.3389/fphys.2017.00718

Cited by 43 publications

(36 citation statements)

References 46 publications

(71 reference statements)

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“…In addition, significant differences in the diversity and richness of bacteria among the GIT regions ( (Gong et al, 2017;Jiao et al, 2016;Mao et al, 2015;Wang et al, 2016). In the present study, all of these factors were consistent for the 12 experimental goats; however, the relative abundances of the dominant phyla and genera varied considerably among the GIT compartments, which emphasized that the sampling site was the major determinant of the microbial composition and community structure along the GIT.…”

Section: Discussionsupporting

confidence: 70%

“…All of our experimental results mentioned above indicated that significant differences in microbial diversity existed among the GIT sections. Previous studies have shown that the bacterial composition of animal GITs is mainly affected by animal species, age, sex, genetics, environment, and the dietary composition (Gong et al., ; Jiao et al., ; Mao et al., ; Wang et al., ). In the present study, all of these factors were consistent for the 12 experimental goats; however, the relative abundances of the dominant phyla and genera varied considerably among the GIT compartments, which emphasized that the sampling site was the major determinant of the microbial composition and community structure along the GIT.…”

Section: Discussionmentioning

confidence: 99%

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Characterizing the bacterial community across the gastrointestinal tract of goats: Composition and potential function

et al. 2019

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The composition and function of the microbial community in the gastrointestinal tract (GIT) have increasingly captured the attention of nutritionists because these traits affect the nutrient utilization efficiency and health of host animals. Little information has been reported on these aspects of the goat GIT. This study used 12 female goats (weighing 20.70 ± 1.60 kg and 10 months of age) to examine the composition and function of the microbiota in the rumen, abomasum, jejunum, cecum, and colon. Total genomic DNA was extracted from chyme samples from different sections of the GIT, and the hypervariable region of the 16S rRNA gene was amplified by PCR using bacterial universal primers. The amplicons were sequenced on an Illumina MiSeq platform, and the biological information was analyzed using QIIME software. A total of 857 genera that belonged to 39 phyla were observed across the goat GIT, with Bacteroidetes and Firmicutes dominating. Our results revealed significant differences in the composition, diversity, and species abundance of the bacterial communities in the different sections of the GIT. However, the compositions of the bacterial communities in adjacent GIT segments showed similarities in addition to differences. The study indicated that there were significant differences in microbial function among the GIT regions. In particular, the relative abundances of genes involved in energy metabolism, amino acid metabolism, nucleotide metabolism, and glycan metabolism were overrepresented in samples from the forestomach, and genes related to energy metabolism, amino acid metabolism, and glycan metabolism were mainly enriched in samples from the small intestine. Additionally, the relative abundances of bacteria at the phylum and genus levels were significantly correlated with these metabolic functions. In general, there were significant differences in composition and potential function among the bacterial communities in the goat GIT.

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Section: Discussionsupporting

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Characterizing the bacterial community across the gastrointestinal tract of goats: Composition and potential function

et al. 2019

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“…Energy availability is essential for sperm motility and this is promoted by the action of the mitochondria located in the intermediate part of the spermatozoid. Hyperthermia may lead to dephosphorylation and activation of glycogen synthase kinase 3 (GSK 3 ), a protein kinase that binds to proteins of the outer mitochondrial membrane and regulates its permeability, reducing its ability to produce energy (Gong et al, ). Thus, any change in mitochondrial function may be reflected in altered sperm motility (Gravance, Garner, Baumber, & Ball, ).…”

Section: Discussionmentioning

confidence: 99%

Influence of post‐thawing thermal environment on bovine sperm characteristics and in vitro fertility

et al. 2019

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Our aim was to evaluate the effects of three thermal environments over time on kinetics, functionality and in vitro fertility of cryopreserved bovine spermatozoa. Four ejaculates from five bulls (n = 20) were cryopreserved. After thawing, semen was evaluated (0 hr), incubated for 4 hr in T36.0 (36.0°C), T38.0 (38.0°C) and T39.5 (39.5°C), and analysed every hour (1 hr, 2 hr, 3 hr, 4 hr). In vitro production of embryos was performed at 0 hr and 4 hr. Sperm motility and cell kinetics (Computer‐Assisted Sperm Analysis) were impaired after 2 hr at T38.0 and T39.5 (p < 0.05). Flow cytometry revealed an increase in the cells with injured plasma membrane to 39.5°C and a general reduction in the mitochondrial potential over time (p < 0.05). In vitro fertility was impaired in all temperatures after 4 hr, but there was no difference between 36.0°C and 38.0°C. Our results suggest that the ex situ resilience of semen at 36.0°C after thawing with no major damage to the quality is limited to 3 hr. In normothermia or in thermal stress, sperm cells present a gradual reduction of movement and functionality, which were more significant after 1 hr of incubation. The in vitro production of embryos is impaired when the semen is kept in a thermal environment ≥36.0°C for 4 hr.

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“…ROS negatively effect on cell metabolism by damaging sperm plasma membrane and fragmentation of both nuclear and mitochondrial DNA [25]. Several studies reported that lower sperm production and motility and high rate of abnormal forms were signi cantly associated with increasing temperature of testis [26][27][28].…”

Section: Discussionmentioning

confidence: 99%

Lifestyle and Occupation patterns with poor semen quality: A cross sectional analysis

Ranasinghe

Gamage

Perera

et al. 2020

Preprint

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Background Infertility is a major problem persisting all around the world. According to WHO the rate of infertility is approximately 15% worldwide and it differ from geographical location, ethnicity and social status. Lifestyle habits, environmental and occupational hazards, physical parameters can be recognized as major risk factors which may affect male infertility. The objective of this study was to determine factors associated with male infertility in Sri Lanka. Methods A cross sectional study was conducted on 299 individuals participated for an infertility clinic in a Teaching hospital in Sri Lanka. Socio-demographic, occupational and environmental characteristics were collected using interviewer administered questionnaire. Semen samples were collected from each participant for laboratory investigations. Sperm concentration and motility, morphology and viability of sperms were measured. Results Out of total participants, 30.1% of participants had a sperm concentration of < 15 × 10⁶and the sperm mortality was < 32% in 34.7% participants. Older age, tobacco smokers, individuals using tight under wears and individuals exposed to either heat or chemical hazards were identified as risk groups with low sperm concentration and low semen volume. In addition, older age, individuals using tight under wears and individuals exposed to either heat or chemical hazards were significantly associated with low or abnormal sperm mortality and morphology. Individuals having diabetes showed a significantly higher non-motility rate of sperms. Alcohol usage, betel chewing, mumps, special radiation exposure, body mass index and waist circumference were not significantly associated with semen parameters. Conclusion Older age, tobacco smoking, wearing tight underwear, occupational exposures, and diabetes mellitus has shown a great risk for the generation of poor semen parameters, which can lead to male infertility. Furthermore it is very important to carry out extended studies regarding this problem to establish the effect of above factors.

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Heat Stress Reduces Sperm Motility via Activation of Glycogen Synthase Kinase-3α and Inhibition of Mitochondrial Protein Import

Cited by 43 publications

References 46 publications

Characterizing the bacterial community across the gastrointestinal tract of goats: Composition and potential function

Characterizing the bacterial community across the gastrointestinal tract of goats: Composition and potential function

Influence of post‐thawing thermal environment on bovine sperm characteristics and in vitro fertility

Lifestyle and Occupation patterns with poor semen quality: A cross sectional analysis

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