Heat stress promotes mitochondrial instability and oxidative responses in yeast deficient in thiazole biosynthesis

Medina-Silva, Renata; Barros, Marcelo P.; Galhardo, Rodrigo S.; Netto, L.E.S.; Colepicolo, Pio; Menck, Carlos Frederico Martins

doi:10.1016/j.resmic.2005.07.004

Cited by 41 publications

(32 citation statements)

References 23 publications

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“…A glycolate oxidase (glcD) oxidoreductase gene was activated by NaCl treatment and a G. hirsutum Gibberellin 20-oxidase 1 ( GA20ox1 ) was up-regulated by pH treatment. Interestingly, a gene encoding thiazole biosynthetic enzyme (GeneBank ID: CO076413) was up-regulated over 18 times by all of the five stress conditions, similar result was also reported in other species such as yeast and sunflower [40,41]. Function of more than 40-50% of the identified stress-responsive genes remained unknown.…”

Section: Resultssupporting

confidence: 66%

Transcriptome Analysis Reveals Crosstalk of Responsive Genes to Multiple Abiotic Stresses in Cotton (Gossypium hirsutum L.)

et al. 2013

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Abiotic stress is a major environmental factor that limits cotton growth and yield, moreover, this problem has become more and more serious recently, as multiple stresses often occur simultaneously due to the global climate change and environmental pollution. In this study, we sought to identify genes involved in diverse stresses including abscisic acid (ABA), cold, drought, salinity and alkalinity by comparative microarray analysis. Our result showed that 5790, 3067, 5608, 778 and 6148 transcripts, were differentially expressed in cotton seedlings under treatment of ABA (1μM ABA), cold (4°C), drought (200mM mannitol), salinity (200mM NaCl) and alkalinity (pH=11) respectively. Among the induced or suppressed genes, 126 transcripts were shared by all of the five kinds of abiotic stresses, with 64 up-regulated and 62 down-regulated. These common members are grouped as stress signal transduction, transcription factors (TFs), stress response/defense proteins, metabolism, transport facilitation, as well as cell wall/structure, according to the function annotation. We also noticed that large proportion of significant differentially expressed genes specifically regulated in response to different stress. Nine of the common transcripts of multiple stresses were selected for further validation with quantitative real time RT-PCR (qRT-PCR). Furthermore, several well characterized TF families, for example, WRKY, MYB, NAC, AP2/ERF and zinc finger were shown to be involved in different stresses. As an original report using comparative microarray to analyze transcriptome of cotton under five abiotic stresses, valuable information about functional genes and related pathways of anti-stress, and/or stress tolerance in cotton seedlings was unveiled in our result. Besides this, some important common factors were focused for detailed identification and characterization. According to our analysis, it suggested that there was crosstalk of responsive genes or pathways to multiple abiotic or even biotic stresses, in cotton. These candidate genes will be worthy of functional study under diverse stresses.

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Section: Resultssupporting

confidence: 66%

Transcriptome Analysis Reveals Crosstalk of Responsive Genes to Multiple Abiotic Stresses in Cotton (Gossypium hirsutum L.)

et al. 2013

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“…This result points to a possible role of sti35 in heat stress response. In agreement with this finding, the thi-4 mutant of S. cerevisiae exhibited increased induction of petite mutants compared to the wild type upon chronic exposure to 37°C, as well as a strong oxidative enzymatic response and protein oxidations, suggesting a potential role for the Thi4 protein in protecting yeast from heat stress (Medina-Silva et al, 2006). Transcription of the N. crassa sti35 orthologue, CyPBP37, which was identified as a protein binding to the cyclophilin NcCy41, was also induced by a variety of stresses including high temperature (Faou and Tropschug, 2004).…”

Section: Regulation Of Sti35 Expression In Culture and In Plantasupporting

confidence: 76%

The Fusarium oxysporum sti35 gene functions in thiamine biosynthesis and oxidative stress response

Ruiz‐Roldán

Puerto-Galán

Roa

et al. 2008

Fungal Genetics and Biology

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“…Specifically, Arabidopsis THI1 protein and THI4 found in yeast appear to be involved in the protection and repair of damaged mitochondrial DNA (Machado et al , 1997; Chabregas et al , 2001). In yeast, a THI4 mutant is more susceptible to oxidative stress under high temperatures even though the cultures were supplemented with thiamine (Medina-Silva et al , 2006). Alternatively, if thiamine were degraded faster at high temperatures, an increase in THI1 may simply be indicative of an increase in thiamine turnover.…”

Section: Discussionmentioning

confidence: 99%

Physiological and molecular changes in Oryza meridionalis Ng., a heat-tolerant species of wild rice

Scafaro¹,

Haynes²,

Atwell³

2009

Journal of Experimental Botany

148

118

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Oryza meridionalis Ng. is a wild relative of Oryza sativa L. found throughout northern Australia where temperatures regularly exceed 35 °C in the monsoon growing season. Heat tolerance in O. meridionalis was established by comparing leaf elongation and photosynthetic rates at 45 °C with plants maintained at 27 °C. By comparison with O. sativa ssp. japonica cv. Amaroo, O. meridionalis was heat tolerant. Elongation rates of the third leaf of O. meridionalis declined by 47% over 24 h at 45 °C compared with a 91% decrease for O. sativa. Net photosynthesis was significantly higher in O. sativa at 27 °C whereas the two species had the same assimilation rates at 45 °C. The leaf proteome and expression levels of individual heat-responsive genes provided insight into the heat response of O. meridionalis. After 24 h of heat exposure, many enzymes involved in the Calvin Cycle were more abundant, while mRNA of their genes generally decreased. Ferredoxin-NADP(H) oxidoreductase, a key enzyme in photosynthetic electron transport had both reduced abundance and gene expression, suggesting light reactions were highly susceptible to heat stress. Rubisco activase was strongly up-regulated after 24 h of heat, with the large isoform having the largest relative increase in protein abundance and a significant increase in gene expression. The protective proteins Cpn60, Hsp90, and Hsp70 all increased in both protein abundance and gene expression. A thiamine biosynthesis protein (THI1), previously shown to act protectively against stress, increased in abundance during heat, even as thiamine levels fell in O. meridionalis.

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Heat stress promotes mitochondrial instability and oxidative responses in yeast deficient in thiazole biosynthesis

Cited by 41 publications

References 23 publications

Transcriptome Analysis Reveals Crosstalk of Responsive Genes to Multiple Abiotic Stresses in Cotton (Gossypium hirsutum L.)

Transcriptome Analysis Reveals Crosstalk of Responsive Genes to Multiple Abiotic Stresses in Cotton (Gossypium hirsutum L.)

The Fusarium oxysporum sti35 gene functions in thiamine biosynthesis and oxidative stress response

Physiological and molecular changes in Oryza meridionalis Ng., a heat-tolerant species of wild rice

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