Heat Stress of Cancer Cells: Fluorescence Imaging of Structural Changes with Quantum Dots 605 and Alexa 488

Minet, Olaf; Dressler, Cathrin; Beuthan, J.

doi:10.1007/3-540-27004-3_16

Cited by 1 publication

(2 citation statements)

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“…Mitochondria respiring without disturbance are in a steady state [10] with the first members of the electron transport chain functioning in a rather reduced state and the last mainly in an oxidized state. Initial preliminary investigations of the outer cell membranes, which are considerably more stable, [11] 2) scattered light applicator, (3) copper diaphragm, (4) optical fiber (diameter 360 µm, UV permeable), ( 5) pyroelectric receiver have shown that these membranes having been subjected to heat stress for a short while are badly damaged, already.…”

Section: Introductionmentioning

confidence: 99%

“…Figure 3Diagrammatic view of the experimental laserspectroscopic setup for in vivo and in vitro measurements on biological samples: (1) sample, (2) fiber, (3) lens, (4) semipermeable mirror, (5) diaphragm, (6) photo diode for triggering the boxcar integrators, (7) grating, (8) interference filter, (9) photoelectric amplifier, (10) nitrogen laser,(11) boxcar integrator…”

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confidence: 99%

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Investigating laser-induced fluorescence of metabolic changes in Guinea pig livers during Nd:YAG laser irradiation

Minet

Beuthan

2004

Laser Phys. Lett.

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Recently, dosimetry of thermal influences on biological tissue has been attracting more and more interest among scientists. For temperatures exceeding 56• C, the irreversible tissue coagulation can be approximately described by the Arrhenius theory, which was established in chemical kinetics. Investigations in the temperature range between 43• C and 55 • C are rare and are, therefore, not available in a systematic form. A Nd:YAG laser (1060 nm) was used, to heat up liver tissue of guinea pigs in this temperature range periodically at intervals of ca. 3 minutes. A second laser (N2, 337 nm) was used to simultaneously excite the autofluorescence of NADH as coenzyme of the cell respiration. The results of the measurement series show strong inter-individual variations of the metabolic decay time. The Arrhenius theory fails to provide a description in this temperature range thus opening up new modeling possibilities.

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Section: Introductionmentioning

confidence: 99%

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confidence: 99%