Heat stress modifies the lactational performances and the urinary metabolomic profile related to gastrointestinal microbiota of dairy goats

Contreras-Jodar, Alexandra; Nayan, Nazri; Hamzaoui, S.; Caja, G.; Salama, A. A. K.

doi:10.1101/385930

Cited by 7 publications

(7 citation statements)

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“…Microbiome sequencing was used to determine changes in the rumen bacterial taxa of Jersey and Holstein cows to heat stress [14]. Several experiments have already been conducted to measure some metabolites of heat stress in dairy goats [15], pigs [16] and dairy cows [17]. In addition, Tian [18] reported 10 biomarkers in milk associated with heat stress-induced metabolomic alterations in blood.…”

Section: Introductionmentioning

confidence: 99%

WITHDRAWN: Novel Insights Into Heat Tolerance Using Microbiome and Metabolomics Analyses in Dairy Cows Rumen Fluid

Liu

Pang

et al. 2021

Preprint

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Background: Heat stress is a key issue of growing concern for livestock industry worldwide due to its negative effects not only on milk production, fertility, health, welfare, and economic returns of dairy cows, but also on the microbial communities in the rumen. However, the underlying relationship between rumen microbiome and its associated metabolism with heat tolerance in cow have not been extensively described yet. Therefore, the main objective of this study was to investigate differential heat resistance in Holstein cows using rumen microbiome and metabolome analyses.Methods: We performed both principal component analysis and membership function analysis to select 7 heat-tolerant (HT) and 7 heat-sensitive (HS) cows. The ruminal fluid samples of two groups were collected at two hours post feeding on 7th day of heat stress period, for analyses including rumen fermentation parameters, rumen microbiome and nontargeted metabolomics.Results: Under heat stress conditions, the HT cows had a significantly higher propionic acid content than the HS cows; whereas measures of the respiratory rate (RR), rectal temperature (RT), acetic,butyric acid and acetic acid to propionic acid ratio (A:P) in the HT cows were lower compared with the HS cows. Omics sequencing revealed that the relative abundance of Rikenellaceae_RC9_gut_group, Succiniclasticum, Ruminococcaceae_NK4A214_group and Christensenellaceae_R-7_group were significantly higher in the HT than HS cows; whereas Prevotella_1, Ruminococcaceae_UCG-014, and Shuttleworthia were significantly higher in the HS cows compared to HT cows. Substances mainly involved in carbohydrate metabolism, including glycerol, mannitol, and maltose, showed significantly higher content in the HT cows compared to that in the HS cows. Simultaneously, RR was significantly correlated with both differential microorganisms and distinct metabolites, suggesting three metabolites could be potential biomarkers for determining heat resistance that require further research.Conclusion: Overall, distinct changes in the rumen microbiome and metabolomics in the HT cows may be associated with better adaptability to heat stress. These findings suggest their use as diagnostic tools of heat tolerance in dairy cattle breeding schemes.

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Section: Introductionmentioning

confidence: 99%

WITHDRAWN: Novel Insights Into Heat Tolerance Using Microbiome and Metabolomics Analyses in Dairy Cows Rumen Fluid

Liu

Pang

et al. 2021

Preprint

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“…The activation of NFkB pathway increases tight junction permeability through expression changes of claudins in the mammary gland 37 . The higher losses of milk lactose detected in HS-LPS can be explained by greater tight junction leakiness, since HS has been shown to compromise tight junction integrity in other tissues such as the gastrointestinal barrier 9,38 .…”

Section: Responses To Intramammary Lps Challenge Under Thermo-neutralmentioning

confidence: 99%

Milk yield, milk composition, and milk metabolomics of dairy goats intramammary-challenged with lipopolysaccharide under heat stress conditions

Salama

Contreras-Jodar

Love

et al. 2020

Sci Rep

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Heat stress and mastitis are major economic issues in dairy production. the objective was to test whether goat's mammary gland immune response to E. coli lipopolysaccharide (LpS) could be conditioned by heat stress (HS). changes in milk composition and milk metabolomics were evaluated after the administration of LpS in mammary glands of dairy goats under thermal-neutral (tn; n = 4; 15 to 20 °C; 40 to 45% humidity) or HS (n = 4; 35 °C day, 28 °C night; 40% humidity) conditions. Milk metabolomics were evaluated using 1 H nuclear magnetic resonance spectroscopy, and multivariate analyses were carried out. Heat stress reduced feed intake and milk yield by 28 and 21%, respectively. Mammary treatment with LpS resulted in febrile response that was detectable in tn goats, but was masked by elevated body temperature due to heat load in HS goats. Additionally, LpS increased milk protein and decreased milk lactose, with more marked changes in HS goats. the recruitment of somatic cells in milk after LpS treatment was delayed by HS. Milk metabolomics revealed that citrate increased by HS, whereas choline, phosphocholine, n-acetylcarbohydrates, lactate, and ß-hydroxybutyrate could be considered as putative markers of inflammation with different pattern according to the ambient temperature (i.e. tn vs. HS). in conclusion, changes in milk somatic cells and milk metabolomics indicated that heat stress affected the mammary immune response to simulated infection, which could make dairy animals more vulnerable to mastitis.The negative effects of heat stress (HS) on the productivity of dairy animals in terms of milk yield, milk composition and milk quality are well documented 1,2 . Despite advances in cooling systems and environmental management, HS constitutes to be a significant cost for the dairy industry 3 . Goats, originated from hot and arid zones, are considered less sensitive to HS compared to cows. However, milk production losses have been reported in heat-stressed dairy goats, especially at early stages of lactation 4,5 .The effect of HS on performance (e.g. milk yield, milk composition, feed intake, body temperature, respiratory rate) has been intensively evaluated in dairy animals 1,2 . However, only a few studies evaluated the omics of biofluids and tissues in animals exposed to HS such as cow's blood plasma 6 , cow's milk 7 , cow's liver 8 and goat's urine 9 .Besides the negative impact of HS on milk production, HS has been found to disrupt the immune function 5 . With regard to mammary immunity during HS, available data indicate that mammary immunity might be compromised by HS. Thompson et al. 10 reported that cows without cooling during the dry period have higher incidence of mastitis in the ensuing lactation. At the systemic level, Contreras-Jodar et al. 5 evaluated the transcriptomics of blood immune cells in heat-stressed goats and detected a decrease in the hematopoiesis and www.nature.com/scientificreports www.nature.com/scientificreports/ leukocyte diapedesis, which might compromise the innate and the adaptive ...

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“…HS causes profound changes in the gut microbiota structure of primiparous sows, especially in the abundance and diversity of some SCFA-producing species ( Singh et al., 2019 ). Many studies conducted on other animals, including broiler chickens ( Wang et al., 2018a ), goats ( Contreras-Jodar et al., 2019 ), ducks ( He et al., 2019 ), and dairy cows ( Chen et al., 2018 ), have also shown that HS affected the composition of the gut microbiota. However, the alteration in the gut microbiota composition and its association with physiological changes induced by HS in pigs has not been well investigated.…”

Section: Introductionmentioning

confidence: 99%

Heat stress-induced mucosal barrier dysfunction is potentially associated with gut microbiota dysbiosis in pigs

Xia

Wei

et al. 2022

Animal Nutrition

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Heat stress (HS) can be detrimental to the gut health of swine. Many negative outcomes induced by HS are increasingly recognized as including modulation of intestinal microbiota. In turn, the intestinal microbiota is a unique ecosystem playing a critical role in mediating the host stress response. Therefore, we aimed to characterize gut microbiota of pigs’ exposure to short-term HS, to explore a possible link between the intestinal microbiota and HS-related changes, including serum cytokines, oxidation status, and intestinal epithelial barrier function. Our findings showed that HS led to intestinal morphological and integrity changes (villus height, serum diamine oxidase [DAO], serum D-lactate and the relative expressions of tight junction proteins), reduction of serum cytokines (interleukin [IL]-8, IL-12, interferon-gamma [IFN-γ]), and antioxidant activity (higher glutathione [GSH] and malondialdehyde [MDA] content, and lower superoxide dismutase [SOD]). Also, 16S rRNA sequencing analysis revealed that although there was no difference in microbial α-diversity, some HS-associated composition differences were revealed in the ileum and cecum, which partly led to an imbalance in the production of short-chain fatty acids including propionate acid and valerate acid. Relevance networks revealed that HS-derived changes in bacterial genera and microbial metabolites, such as Chlamydia, Lactobacillus, Succinivibrio, Bifidobacterium, Lachnoclostridium, and propionic acid, were correlated with oxidative stress, intestinal barrier dysfunction, and inflammation in pigs. Collectively, our observations suggest that intestinal damage induced by HS is probably partly related to the gut microbiota dysbiosis, though the underlying mechanism remains to be fully elucidated.

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Heat stress modifies the lactational performances and the urinary metabolomic profile related to gastrointestinal microbiota of dairy goats

Cited by 7 publications

References 40 publications

WITHDRAWN: Novel Insights Into Heat Tolerance Using Microbiome and Metabolomics Analyses in Dairy Cows Rumen Fluid

WITHDRAWN: Novel Insights Into Heat Tolerance Using Microbiome and Metabolomics Analyses in Dairy Cows Rumen Fluid

Milk yield, milk composition, and milk metabolomics of dairy goats intramammary-challenged with lipopolysaccharide under heat stress conditions

Heat stress-induced mucosal barrier dysfunction is potentially associated with gut microbiota dysbiosis in pigs

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