Heat Stress Induction of Carrot Somatic Embryogenesis.

Kamada, Hiroshi; Tachikawa, Yoshinobu; Saitou, Tsutomu; Harada, Hiroshi

doi:10.5511/plantbiotechnology1984.11.229

Cited by 36 publications

(22 citation statements)

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“…Some somatic cells in plants can restart embryogenesis in vitro when they are exposed to a wide range of severe abiotic stressors (Fehér, 2014 Kamada et al, 1989Kamada et al, , 1993Kamada et al, , 1994, and similar stress-induced embryogenesis has also been reported in Arabidopsis (Ikeda-Iwai et al, 2003). Many plant species also undergo somatic embryogenesis when they are cultured on auxin-containing medium and then transferred to auxin-free medium (Wernicke and Brettell, 1980;Lu et al, 1983;Ikeda-Iwai et al, 2002).…”

Section: Molecular Basis Of Somatic Embryogenesismentioning

confidence: 99%

Plant regeneration: cellular origins and molecular mechanisms

et al. 2016

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Compared with animals, plants generally possess a high degree of developmental plasticity and display various types of tissue or organ regeneration. This regenerative capacity can be enhanced by exogenously supplied plant hormones in vitro, wherein the balance between auxin and cytokinin determines the developmental fate of regenerating organs. Accumulating evidence suggests that some forms of plant regeneration involve reprogramming of differentiated somatic cells, whereas others are induced through the activation of relatively undifferentiated cells in somatic tissues. We summarize the current understanding of how plants control various types of regeneration and discuss how developmental and environmental constraints influence these regulatory mechanisms.

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Section: Molecular Basis Of Somatic Embryogenesismentioning

confidence: 99%

Plant regeneration: cellular origins and molecular mechanisms

et al. 2016

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“…3-A). [9][10][11][12]17]. These results indicate that these proteins may be directly related to induction of somatic embryogenic competence and/or early events of somatic embryo development prior to morphological differentiation of embryos rather than stress tolerance.…”

Section: Discussionmentioning

confidence: 80%

“…Futhermore, heat stress also induces somatic embryogenesis in phytohormone -free medium (Kamada et al, 1994) [12]. In these methods, after the segments were transferred to phytohormonefree medium without these substances or high temperature, somatic embryos formed directly on the surface of the explants without visible callus formation.…”

Section: Introductionmentioning

confidence: 99%

Changes in Protein Pattern during Stress-induction of Carrot (Daucus carota L.) Somatic Embryogenesis.

Tachikawa

Saitou

Kamada

et al. 1998

Plant Biotechnology

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To clarify the molecular biological aspects occurring during the induction of somatic embryogenesis, the stress-induced changes in protein pattern and the proteins associated with somatic embryogenesis induced by stress in carrot (Daucus carota L.) were investigated. In two dimensional polyacrylamide gel electrophoresis (2D-PAGE), 6 spots with molecular weight of 63-65 kilo Dalton (KDa) showing pI ranging from 6.0-6.5, and 2 spots of molecular weight 45 KDa with a pI of 5.0 were detected in the extracts of apical tip segments treated with cadmium chloride, sucrose and sodium chloride. Three spots out of the 6 spots of 63-65 KDa molecular weight and the two 45 KDa spots were also detected in the extracts of 2,4-dichlorophenoxy acetic acid (2,4-D) -induced embryogenic cells, but not in those of non-embryogenic cells. Furthermore, these 5 spots were also detected in the extracts of hypocotyl segments cultured with 2,4-D for more than 4weeks. Judging from the relationship between accumulation of these proteins and acquisition of embryogenic competence, these proteins may be related to acquisition and/or maintenance of embryogenic competence.

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“…Although plant growth regulators are generally used for the induction of somatic embryos, it was reported that somatic embryos were induced in carrot without plant growth regulators. Explants exposed to a high osmotic pressure (Kamada et a!.,1993), NaCI (Kiyosue et al, 1989a), heavy metals (Kiyosue et al, 1990), hypochrolite (Kiyosue et al, 1989b) or high temperature (Kamada et al, 1994) formed somatic embryos. Those authors concluded that somatic embryogenesis was induced by various kinds of stress.…”

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confidence: 99%

Osmotic and Non-osmotic Induction of Somatic Embryogenesis by Sucrose at High Concentrations in Daucus carota L.

Satoh

Ooka

Wakai

et al. 2000

Plant Biotechnology

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In the detached cotyledons of carrot, Daucus carota L., somatic embryogenesis was induced by 2-week treatment with sucrose at 0.29-0.73 M both in the epidermal cells (type-1) and the callus-like cells generated at the cut end of the cotyledons (type -2). Type-2 embryos were produced predominantly, after the treatment with 0.29-0.44 M sucrose and type-1 embryos after treatment with 0.58-0.73 M sucrose. Sucrose and glucose were more effective than mannitol and sorbitol for the induction of somatic embryogenesis. Furthermore, both type-1 and type-2 embryos were induced by sucrose and glucose, but only type -2 embryos by mannitol and sorbitol. These results suggest that sucrose does not work as a mere osmoticum.

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Heat Stress Induction of Carrot Somatic Embryogenesis.

Cited by 36 publications

References 0 publications

Plant regeneration: cellular origins and molecular mechanisms

Plant regeneration: cellular origins and molecular mechanisms

Changes in Protein Pattern during Stress-induction of Carrot (Daucus carota L.) Somatic Embryogenesis.

Osmotic and Non-osmotic Induction of Somatic Embryogenesis by Sucrose at High Concentrations in Daucus carota L.

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