Heat-stress induced inhibition in growth and chlorosis in mungbean (Phaseolus aureus Roxb.) is partly mitigated by ascorbic acid application and is related to reduction in oxidative stress

Abstract

The rising temperatures ([35°C) are proving detrimental to summer-sown mungbean genotypes that experience inhibition of vegetative and reproductive growth. In the present study, the mungbean plants growing hydroponically at varying temperatures of 30/20°C (control), 35/25, 40/30, and 45/35°C (as day/night 12 h/12 h) with (50 lM) or without ascorbic acid (ASC) were investigated for effects on growth, membrane damage, chlorophyll loss, leaf water status, components of oxidative stress, and antioxidants. The ASC-treated plants showed significant improvement in germination and seedling growth especially at 40/30 and 45/35°C. The damage to membranes, loss of water, decrease in cellular respiration, and chlorophyll were significantly prevented by ASC treatment to plants growing at these temperatures. The oxidative stress measured as malondialdehyde and hydrogen peroxide content was observed to be significantly lower at high temperatures with ASC application. The activities of superoxide dismutase, catalase, ascorbate peroxidase, and glutathione reductase increased at 40/30°C but decreased at 45/35°C in the absence of ASC while with its application, the activities of these enzymes were appreciably resorted. Among all the antioxidants, the endogenous ASC content decreased to the greatest extent at 45/35°C grown plants indicating its vital role in affecting the response of mungbean to heat stress. Exogenously applied ASC raised its endogenous content along with that of glutathione and proline at 45/35°C. The findings indicated that heat stress-induced inhibition in growth and chlorosis was associated with decrease in leaf water status and elevation of oxidative stress, which could partly be prevented by exogenous application of ASC. Its role in imparting protection against heat stress is discussed.

Influence of heat stress on leaf morphology and nitrogen–carbohydrate metabolisms in two wucai (Brassica campestris L.) genotypes

“…In our study, heat stress significantly hampered the shoot growth and foliage expansion in WS-1 (Tab. 1), which was consistent with the previous report in mung bean and wheat [27,28]. The reduction of WS-1 was apparently lower than in WS-6.…”

Exogenous Application of Ascorbic Acid Ameliorates Detrimental Effects of Salt Stress in Rice (MRQ74 and MR269) Seedlings

“…Its intracellular concentration can build up to the millimolar range, e.g., 20-30 mM in the chloroplast stroma and approximately 20 mM in the cytosol (Noctor and Foyer, 1998). This finding was in agreement with El-Ghamriny et al (1999), Farahat et al (2007, Nahed et al (2009), Kumar et al (2011) and Midan and Sorial (2011) as well as Aly et al (2012) in Trifolium alexandrinum L. When the medium was supplemented with ascorbic acid, despite the concentration levels, the activity of acid phosphatase was improved and increased. It has been well documented that salt stress increases acid phosphatase activity (Arab and Ehsanpour, 2006).…”

Control of cucumber (Cucumis sativus L.) tolerance to chilling stress—evaluating the role of ascorbic acid and glutathione

“…The oxidative and reductive status of AsA and GSH are closely related to the adaptation of plants to stressed environments, and the accomplishment of AsA function depends largely on the available GSH supply and the conditions of oxidation and reduction in cells (Anjum et al, 2010). Lowered extent of oxidative stress and enhanced stress resistance in plants has also been reported with exogenous application (seed treatment, soil influx or foliar spraying) of AsA (Dolatabadian et al, 2008;Dolatabadian and Saleh, 2009;Al-Hakimi and Hamada, 2011;Kumar et al, 2011b) or GSH (Cai et al, 2010;Teh et al, 2014).…”

“…Though the role of AsA and GSH in plant stress-tolerance has been emphasized (Anjum et al, 2010;Noctor et al, 2012), and the literature is full on the significance of AsA (Dolatabadian et al, 2008;Kumar et al, 2011b;Zhang et al, 2011), and also that of GSH (Anjum et al, 2010;Cai et al, 2010), reports on a comparative account of AsA and GSH in chilling exposed plants are rare and/or unsubstantiated. Therefore, this work analyzes the role of exogenously applied AsA and GSH in the alleviation of chilling stress-impact in cucumber (Cucumis sativus L. cv.…”