Heat stress during grain filling regulates seed germination through alterations of DNA methylation in barley (Hordeum vulgare L.)

Sakai, Yuki; Suriyasak, Chetphilin; Inoue, Miki; Hamaoka, Norimitsu; Ishibashi, Yoshihiro

doi:10.1007/s11103-022-01278-5

Cited by 12 publications

(6 citation statements)

References 34 publications

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“…Mutations in MSD , such as the oiwa mutant, disrupt ROS homeostasis, resulting in high ROS levels in the embryo sac and leading to sterility or arrested embryogenesis (Victoria Martin et al 2013 ). Recently, there have been reports highlighting the crucial roles of DNA methylation-mediated ROS homeostasis in various aspects of plant development and stress response, including salt stress (Chen et al 2015 ; Hu et al 2021 ), heat stress(Ma et al 2018 ; Sakai et al 2022 ; Zhu et al 2021 ), chilling/freezing stress(Guo et al 2019 ; Zheng et al 2022 ), and fruit ripening (He et al 2020 ). For instance, the application of 5-azaC, an inhibitor of DNA methyltransferase, has been found to enhance the rates of superoxide anion (O 2 − ) production, thereby contributing to the ripening of berry fruits (Guo et al 2019 ).…”

Section: Introductionmentioning

confidence: 99%

DNA methylation-mediated ROS production contributes to seed abortion in litchi

Xie,

Zheng,

Xue

et al. 2024

Mol Horticulture

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Although there is increasing evidence suggesting that DNA methylation regulates seed development, the underlying mechanisms remain poorly understood. Therefore, we aimed to shed light on this by conducting whole-genome bisulfite sequencing using seeds from the large-seeded cultivar 'HZ' and the abortive-seeded cultivar 'NMC'. Our analysis revealed that the 'HZ' seeds exhibited a hypermethylation level compared to the 'NMC' seeds. Furthermore, we found that the genes associated with differentially methylated regions (DMRs) and differentially expressed genes (DEGs) were mainly enriched in the reactive oxygen species (ROS) metabolic pathway. To investigate this further, we conducted nitroblue tetrazolium (NBT) and 2,7-Dichlorodihydrofluorescein (DCF) staining, which demonstrated a significantly higher amount of ROS in the 'NMC' seeds compared to the 'HZ' seeds. Moreover, we identified that the gene LcGPX6, involved in ROS scavenging, exhibited hypermethylation levels and parallelly lower expression levels in 'NMC' seeds compared to 'HZ' seeds. Interestingly, the ectopic expression of LcGPX6 in Arabidopsis enhanced ROS scavenging and resulted in lower seed production. Together, we suggest that DNA methylation-mediated ROS production plays a significant role in seed development in litchi, during which hypermethylation levels of LcGPX6 might repress its expression, resulting in the accumulation of excessive ROS and ultimately leading to seed abortion.

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Section: Introductionmentioning

confidence: 99%

DNA methylation-mediated ROS production contributes to seed abortion in litchi

Xie,

Zheng,

Xue

et al. 2024

Mol Horticulture

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“…Another drought stress gene KCS encoded by HORVU2Hr1G094090 enhanced drought tolerance, increased the amount of chloroplast matrix, and increased stem diameter, stem coat thickness, growth rate, and lignin content in jute ( Zhang et al., 2019 ; Tong et al., 2021 ). Moreover, two potential candidate genes (HORVU4Hr1G076460 and HORVU4Hr1G076250) were found next to each other on 4H, HORVU4Hr1G076460 encodes RBOH, DNA methylation of genes related to seed development affected by heat stress during grain filling ( Mahalingam et al., 2021 ; Sakai et al., 2022 ). Brassinosteroid signaling kinases (BSK) encoded by HORVU4Hr1G076250 is a key family of receptor-like cytoplasmic kinases for BR signaling ( Li et al., 2022 ), which is crucial for the development of plants, immunity and abiotic stress response, but these genes still are needed further verification in barley.…”

Section: Discussionmentioning

confidence: 99%

Identification of QTL underlying the main stem related traits in a doubled haploid barley population

Zhang

Zhao

et al. 2022

Front. Plant Sci.

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Lodging reduces grain yield in cereal crops. The height, diameter and strength of stem are crucial for lodging resistance, grain yield, and photosynthate transport in barley. Understanding the genetic basis of stem benefits barley breeding. Here, we evaluated 13 stem related traits after 28 days of heading in a barley DH population in two consecutive years. Significant phenotypic correlations between lodging index (LI) and other stem traits were observed. Three mapping methods using the experimental data and the BLUP data, detected 27 stable and major QTLs, and 22 QTL clustered regions. Many QTLs were consistent with previously reported traits for grain filling rate, internodes, panicle and lodging resistance. Further, candidate genes were predicted for stable and major QTLs and were associated with plant development and adverse stress in the transition from vegetative stage to reproductive stage. This study provided potential genetic basis and new information for exploring barley stem morphology, and laid a foundation for map-based cloning and further fine mapping of these QTLs.

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“…In Arabidopsis, a study investigating the role of histone H1 in repressing heterochromatic transposable elements (TEs) in response to HS reported that H1 functions differently in TE repression depending on the specific location of TEs and confirmed that H1 and DNA methylation suppresses HSmediated responses in plants (Liu et al, 2021c). DNA methylation changes in barley subjected to HS (25 C) during grain filling regulated seed germination, with the promoters of genes involved in ABA biosynthesis (9-cis-epoxycarotenoid dioxygenase, NCED) hypermethylated and those related to ABA catabolism (abscisic acid 8 0 -hydroxylase, ABA'8OH), gibberellic acid biosynthesis (gibberellin oxidase, GA20ox), and NADPH oxidase (Rboh) hypo-methylated in HS-treated seeds (Sakai et al, 2022). (Zeng et al, 2019).…”

Section: Epigenomicsmentioning

confidence: 99%

Temperature‐smart plants: A new horizon with omics‐driven plant breeding

Raza,

Bashir,

Khare

et al. 2024

Physiologia Plantarum

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The adverse effects of mounting environmental challenges, including extreme temperatures, threaten the global food supply due to their impact on plant growth and productivity. Temperature extremes disrupt plant genetics, leading to significant growth issues and eventually damaging phenotypes. Plants have developed complex signaling networks to respond and tolerate temperature stimuli, including genetic, physiological, biochemical, and molecular adaptations. In recent decades, omics tools and other molecular strategies have rapidly advanced, offering crucial insights and a wealth of information about how plants respond and adapt to stress. This review explores the potential of an integrated omics‐driven approach to understanding how plants adapt and tolerate extreme temperatures. By leveraging cutting‐edge omics methods, including genomics, transcriptomics, proteomics, metabolomics, miRNAomics, epigenomics, phenomics, and ionomics, alongside the power of machine learning and speed breeding data, we can revolutionize plant breeding practices. These advanced techniques offer a promising pathway to developing climate‐proof plant varieties that can withstand temperature fluctuations, addressing the increasing global demand for high‐quality food in the face of a changing climate.

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Heat stress during grain filling regulates seed germination through alterations of DNA methylation in barley (Hordeum vulgare L.)

Cited by 12 publications

References 34 publications

DNA methylation-mediated ROS production contributes to seed abortion in litchi

DNA methylation-mediated ROS production contributes to seed abortion in litchi

Identification of QTL underlying the main stem related traits in a doubled haploid barley population

Temperature‐smart plants: A new horizon with omics‐driven plant breeding

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