Heat stress aggravates oxidative stress, apoptosis, and endoplasmic reticulum stress in the cerebellum of male C57 mice

Oghbaei, Hajar; Hosseini, Leila; Farajdokht, Fereshteh; Aghsan, Sepideh Rahigh; Majdi, Alireza; Sadigh-Eteghad, Saeed; Shotorbani, Siamak Sandoghchian; Mahmoudi, Javad

doi:10.1007/s11033-021-06582-9

Cited by 13 publications

(7 citation statements)

References 37 publications

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“…However, HSP70 has the limited protective ability for the body. Excessive HSP70 destroys cell membranes and causes cell injury [ 35 , 36 ]. The decrease in HSP70 expression in cells treated with quercetin and kaempferol indicated that these compounds aid in resisting heat stress and blocking cell injury caused by the excessive increase of HSP70 to a certain extent.…”

Section: Resultsmentioning

confidence: 99%

Probing the Potential Mechanism of Quercetin and Kaempferol against Heat Stress-Induced Sertoli Cell Injury: Through Integrating Network Pharmacology and Experimental Validation

Liu¹,

Liu²,

Hu³

et al. 2022

IJMS

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Quercetin and kaempferol are flavonoids widely present in fruits, vegetables, and medicinal plants. They have attracted much attention due to their antioxidant, anti-inflammatory, anticancer, antibacterial, and neuroprotective properties. As the guarantee cells in direct contact with germ cells, Sertoli cells exert the role of support, nutrition, and protection in spermatogenesis. In the current study, network pharmacology was used to explore the targets and signaling pathways of quercetin and kaempferol in treating spermatogenic disorders. In vitro experiments were integrated to verify the results of quercetin and kaempferol against heat stress-induced Sertoli cell injury. The online platform was used to analyze the GO biological pathway and KEGG pathway. The results of the network pharmacology showed that quercetin and kaempferol intervention in spermatogenesis disorders were mostly targeting the oxidative response to oxidative stress, the ROS metabolic process and the NFκB pathway. The results of the cell experiment showed that Quercetin and kaempferol can prevent the decline of cell viability induced by heat stress, reduce the expression levels of HSP70 and ROS in Sertoli cells, reduce p-NF-κB-p65 and p-IκB levels, up-regulate the expression of occludin, vimentin and F-actin in Sertoli cells, and protect cell structure. Our research is the first to demonstrate that quercetin and kaempferol may exert effects in resisting the injury of cell viability and structure under heat stress.

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Section: Resultsmentioning

confidence: 99%

Probing the Potential Mechanism of Quercetin and Kaempferol against Heat Stress-Induced Sertoli Cell Injury: Through Integrating Network Pharmacology and Experimental Validation

Liu¹,

Liu²,

Hu³

et al. 2022

IJMS

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“…The HS triggers excessive amounts of oxidative stress, resulting in an imbalance between oxidant and antioxidant systems, thereby supporting cellular and organelles damages in animals (Oghbaei et al, 2021;Ma et al, 2022;Miao et al, 2022). Using natural molecules with robust antioxidants is a vital strategy for manipulating the antioxidant defense in stressed rabbits.…”

Section: Discussionmentioning

confidence: 99%

“…GRP78 is indispensable in cellular signaling, immunity, and inflammation pathways (Ibrahim et al, 2019). The upregulation of GRP78 was induced by SH, which plays a critical role in the cellular membrane and intercedes the vast amount of unfolding proteins (Oghbaei et al, 2021). Chronic HS-persuaded ER stress also augmented the generations of misfolded proteins, leading to an increase in misfolded protein synthesis and degradation, as well as vesicle transport ailment (Tang et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

“…The ER is the main place for synthesizing, folding, amendment, and developing secretory and membrane proteins, hence important functions in regulating many cellular developments (Oghbaei et al, 2021). Studies have informed that the environmental stressor could interrupt ER homeostasis, thus resulting in ER stress (Oghbaei et al, 2021;Miao et al, 2022). At normal physiological state in the cell, GRP78 is the dominant of the unfolded protein response (UPR) in the ER, which strengthens it to degrade or refold via cellular degradation machinery (Ibrahim et al, 2019).…”

mentioning

confidence: 99%

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Use of Zinc Nanoparticles and/or Prodigiosin to Mitigate Heat Stress in Rabbits

Aldal’in

Al-Otaibi

Alaryani

et al. 2023

Annals of Animal Science

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Heat stress (HS) is an environmental challenge affecting animals' health, productivity and welfare. This work aimed to inspect the protective effect of zinc oxide nanoparticles (ZnNPs) and/or prodigiosin (PRG) against inflammation, immune dysfunction, oxidative stress and endoplasmic reticulum (ER) stress triggered by HS in growing rabbits. Growing weaned rabbits (one hundred males, 35 days of age) were randomly assigned into four groups. The first group fed a basal diet without supplementation and the 2nd, 3rd, and 4th groups fed diets containing zinc oxide nanoparticles (ZnNPs, 50mg/kg diet), prodigiosin (PRG, 100 mg/kg diet) or their mixture (ZnPRG) under HS conditions for eight successive weeks. The dietary inclusion with ZnNPs and/or PRG significantly boosted FBW (final body weight), CBWG (cumulative body weight gain), and FCR (feed conversion ratio) and had no substantial impacts on the CFI (cumulative feed intake) as compared with those in HS one. All supplemented treatments significantly unveiled an increase in the values of RBCs, hemoglobin, and platelets and significantly decreased in WBCs, basophils and monocytes with non-statistically effects on hematocrit, MCV, MCHC, neutrophils and eosinophils. Compared with the HS group, all supplemental groups showed a significant reduction in TNFα, IL4, IFN-γ, TLR-4 and amyloid A levels and DNA damage markers (Ohdg) and significantly increased in the levels of NO and lysosome activity. Rabbits in the ZnPRG group had significantly higher Ig G and Ig M levels than in other groups. The highest value of CAT and GSH levels were found in rabbits received the mixture of ZnNPs (50mg) and PRG (100mg) in their diets under elevated temperatures. Additionally, both treatments, PRG and ZnNPS, significantly (P<0.001) reduced the values of MDA and MYO, while all treated groups had significantly reduced PC contents compared with the HS group. Co-supplement with ZnPRG showed a considerable restoration in the higher immune expression of reticulum oxidative such GRP78 and IRE1 in hepatic tissues induced by HS conditions. The mixture of ZnNPs and PRG presented more robust effects in mitigating the adverse impacts of HS in rabbits compared with the individual treatments. Collectively, ZnNPs and/or PRG alleviated oxidative stress and DNA damage. In addition, it enhanced the antioxidant capacity and immune function, and downregulated ER stress such as GRP78 and IRE1 signaling in the hepatic tissues of stressed rabbits.

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N‐Acetylcysteine assists muscle development in offspring of mice subjected to maternal heat stress during pregnancy

Lu,

Zhao,

Ding

et al. 2024

J Sci Food Agric

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BACKGROUNDHeat stress (HS) has been shown to affect reproductive performance and muscle development negatively in animals. N‐Acetylcysteine (NAC) plays a pivotal role in enhancing the antioxidant performance in animals as a recognized antioxidant. The present study assesses the potential of NAC to modulate the reproductive performance and antioxidant function in pregnant mice exposed to HS. The role of NAC in muscle development of offspring mice was also explored.RESULTSThe results showed that NAC supplementation from day 12 to day 18 of gestation increased the number of litters and enhanced the antioxidant function in pregnant mice under HS exposure. It improved the weight and body condition significantly in the offspring mice (P < 0.05). The alleviation of HS‐induced muscle impairment with NAC was consistent with the alleviation of apoptosis, the enrichment of the proliferation and differentiation in the offspring mice muscle. N‐Acetylcysteine also reversed HS‐induced reduction in the cross‐sectional area of the leg muscle and increased the proportion of myosin heavy chain IIx (MYHCIIx) in the muscle fiber.CONCLUSIONThe results of the present study support the use of NAC at a dose of 100 mg kg−1 body weight as supplement for protecting the offspring derived from pregnant mice exposed to HS from muscle impairment by accelerating proliferation and differentiation. © 2024 Society of Chemical Industry.

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Heat stress aggravates oxidative stress, apoptosis, and endoplasmic reticulum stress in the cerebellum of male C57 mice

Cited by 13 publications

References 37 publications

Probing the Potential Mechanism of Quercetin and Kaempferol against Heat Stress-Induced Sertoli Cell Injury: Through Integrating Network Pharmacology and Experimental Validation

Probing the Potential Mechanism of Quercetin and Kaempferol against Heat Stress-Induced Sertoli Cell Injury: Through Integrating Network Pharmacology and Experimental Validation

Use of Zinc Nanoparticles and/or Prodigiosin to Mitigate Heat Stress in Rabbits

N‐Acetylcysteine assists muscle development in offspring of mice subjected to maternal heat stress during pregnancy

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