Heat Sterilised Reducing Sugars and Their Effects on the Thermal Resistance of Bacteria

Comparison of two commercial preparations of buffered peptone water for the recovery and growth ofSalmonellabacteria from foods

“…Media preparation, particularly autoclaving and over heating, is an important aspect of culture media that is often overlooked but which may adversely affect medium performance and reliability. During the autoclaving process, auto‐oxidation of phosphate buffers and sugars may potentially occur ( Baumgartner 1938), resulting in the subsequent generation of toxic oxygen species, particularly hydrogen peroxide, within the medium ( Mackey and Derrick 1986). Consequently, poor recovery performance of sub‐lethally injured cells in a particular medium, as observed in this study, may result.…”

The Thermophilic Aerobic Sporeforming Bacteria

“…He observed a generation time at 55 C of ca. 16 minutes, using as experimental material a facultatively thermophilic organism belonging to Cameron and Esty's Group 80 (32). From their description of this group, and from the limiting pH of 4.7 for growth of the bacterium, it may be tentatively identified as B. coagulans, though it might be an especially acid resistant strain of the B. subtilis group.…”

Physical Methods of Sterilization of Microörganisms

“…Fay obtained only 2.5% survivors of E8cherichia coli upon heating immediately after suspending the cells in 50% sucrose, whereas the same heating left 79% of the cells unharmed if they had been in the sugar solution for 2 hours before heating. 6.4' Data of Baumgartner, 1938. Some inconsistencies in the results by various investigators are explainedby Baumgartner (1938) as being caused by harmful decomposition products'originating from reducing sugars during autoclaving. This is prevented by aseptic filtration.…”