“…Enzymatic Time Temperature Integrators (TTI) have been developed (Tucker, 1999;Tucker, Lambourne, Adams, & Lach, 2002) which consist of a thermally labile enzyme whose thermal kinetic closely matches that of target organisms such as Clostridium botulinum (Fellows, 2000). Determination of the degree of degradation of the enzyme at the end of the thermal process enables the integrated temperature history to be obtained (Van Loey, Hendrickx, De Cordt, Haentjens, & Tobback, 1996).…”