Heat shock response in Ceratitis capitata

Stephanou, G.; Alahiotis, S. N.; Marmaras, Vassilis J.; Christodoulou, C.

doi:10.1016/0305-0491(83)90205-5

Cited by 13 publications

(11 citation statements)

References 15 publications

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“…The mechanisms used by insects to survive at high temperature are not well understood. One explanation is the production of heat shock proteins (hsps), which are associated with increased thermotolerance (Alahiotis & Stephanou, 1982; Stephanou et al ., 1983; Berger, 1984; Benedict et al ., 1991; Feeder et al ., 1997). In selection studies with the fruit fly, Drosophila melanogaster , cold‐selected strains were more sensitive to heat killing and produced lower levels of hsps than heat‐tolerant strains (Alahiotis & Stephanou, 1982).…”

Section: Discussionmentioning

confidence: 99%

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Temperature‐related duration of aquatic stages of the Afrotropical malaria vector mosquito Anopheles gambiae in the laboratory

Bayoh

Lindsay

2004

Medical Vet Entomology

187

142

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Vector abundance is an important factor governing disease risk and is often employed when modelling disease transmission. The longevity of the aquatic stages of mosquitoes (Diptera: Culicidae) dictates the rate of production of adults and hence the intensity of disease transmission. We examined how temperature influences the survival of larval stages (larvae and pupae) of Anopheles gambiae Giles sensu stricto and subsequent adult production of this most efficient malaria vector. Groups of 30 mosquitoes were reared at constant temperatures (from 10 to 40 degrees C) from the first instar and observed until death or metamorphosis of the last individual. Larvae developed into adults at temperatures ranging from 16 to 34 degrees C. Larval survival was shortest (< 7 days) at 10-12 degrees C and 38-40 degrees C, and longest (> 30 days) at 14-20 degrees C. Within the temperature range at which adults were produced, larval mortality was highest at the upper range 30-32 degrees C, with death (rather than adult emergence) representing over 70% of the terminal events. The optimal survival temperatures were lower than the temperatures at which development was quickest, suggesting a critical relationship between temperature and the life cycle of the insect. These data provide fundamental information about An. gambiae s.s. adult productivity at different temperatures, which may facilitate the construction of process-based models of malaria risk in Africa and the development of early warning systems for epidemics.

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Section: Discussionmentioning

confidence: 99%

“…Also when D. melanogaster cells were treated with ecdysone, which induces synthesis of small hsps, thermotolerance was increased (Berger, 1984). Medflies, Ceratitis capitata , reared at higher temperatures were found to possess higher levels of hsps and thermotolerance than larvae not pre‐shocked (Stephanou et al ., 1983).…”

Section: Discussionmentioning

confidence: 99%

Temperature‐related duration of aquatic stages of the Afrotropical malaria vector mosquito Anopheles gambiae in the laboratory

Bayoh

Lindsay

2004

Medical Vet Entomology

187

142

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“…'Author to whom all correspondence should be addressed. and salivary glands of Ceratitis capitata (Stephanou et al 1983), fat body (Whyard et al 1986) and epidermis (Baldaia et al 1987) of Locusta migratoria, and cultured cells of A edes albopicrus (Carvalho and Rebel lo 1 987). Several stress-related physiologicaf responses in insects may be centered in the nervous system (Hayakawa et al 1987a(Hayakawa et al , 1987b.…”

Section: Introductionmentioning

confidence: 99%

Examination of heat shock gene expression in nerve cord isolated from heat-stressed American cockroach, Periplaneta americana

Ruder¹,

Ovsenek²,

Heikkila³

et al. 1989

Biochem. Cell Biol.

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Exposure of adult female cockroaches to temperatures ranging from 37 to 42 °C induced the synthesis of a set of heat shock proteins (HSPs) in isolated nerve cord. The major HSPs have molecular masses of 83 000, 70 000, 68 000, and 28 000 daltons. Enhanced synthesis of these HSPs was detectable after 15 min of heat shock and approached maximum levels after 2 h. In cockroaches exposed to 42 °C for 1 h and then returned to 22 °C, a normal pattern of protein synthesis was established within 3–5 h. Northern hybridization analysis using Drosophila HSP 70 and HSP 83 genomic clones revealed the accumulation of 2.6 and 3.1 kilobases mRNA, respectively, in total RNA derived from heat-shocked tissue. A rapid decay of these mRNAs was observed in cockroach nerve cord during recovery at 22 °C following a 1-h heat shock at 42 °C. The decrease in HSP synthesis and HSP mRNA levels during recovery may be due to a decrease in mRNA stability as well as transcription.Key words: heat shock proteins, Periplanata americana, nerve cord, gene expression, mRNA.

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“…Interestingly, we no found evidence in our study of the low M, hsps (20,000; 14,000; 13,000; and 12,000) which they found to be present in their study, even when we electrophoresed proteins on 12.5% and 15% gels (data not shown). These low M, hsps were reported to be variable in their expression in C. capitafa salivary glands and somewhat temperature dependent [19]. These hsps may also be developmental or tissue specific for salivary glands, Joplin and Denlinger [24] found developmental and tissue specificity of hsps in Sarcophgu.…”

Section: Discussionmentioning

confidence: 95%

“…Hsps with similar M, have been found to occur in many other insect systems, especially the 69,000-70,000 hsp found in Drosophilu [13] and Surcophugu [6]. Stephanou et al [19] reported that isolated salivary glands from C. cupituta larvae produced at least eight hsps when exposed to 37°C for 30 min. The 69,000, 87,000, and 34,000 hsps reported by Stephanou et al 1191 correspond closely with the three hsps reported in this study.…”

Section: Discussionmentioning

confidence: 95%

Heat shock proteins and thermotolerance in a cultured cell line from the Mediterranean fruit fly, Ceratitis capitata

Jang

1992

Arch Insect Biochem Physiol

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Heat shock proteins (hsps) were identified in a cell line from the Mediterranean fruit fly, Ceratitis capitata Wiedemann (Diptera: Tephritidae) exposed to elevated temperatures. Cells produced three hsps (Mr 87,000, 69,000, and 34,000) in response to a temperature shift from 26 degrees C to 37 degrees C (30-60 min) with a concomitant decrease in synthesis of most other cellular proteins. Synthesis of low Mr hsps was not evident. The heat shock response is triggered within 30 min at temperatures from 33 degrees C to 41 degrees C. At temperatures greater than 41 degrees C protein synthesis was shut down. Within 2-3 h after return to 26 degrees C, synthesis of proteins repressed at the higher temperatures resumed production while the major hsps disappear. Heat shock proteins were not produced in the presence of actinomycin D. Evaluations on the role of hsps in conferring thermotolerance to the cells showed an increase in cell viability in heat-shocked cells over non-heat-shocked cells (after 3 and 10 days) when subsequently placed at 45 degrees C for 1 h, a normally lethal temperature. Heat shock alone had little effect on subsequent cell viability or growth at 26 degrees C. These results suggest that hsps produced by these cells may aid in the maintenance of cell integrity and thus play a transitory role in thermotolerance.

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Heat shock response in Ceratitis capitata

Cited by 13 publications

References 15 publications

Temperature‐related duration of aquatic stages of the Afrotropical malaria vector mosquito Anopheles gambiae in the laboratory

Temperature‐related duration of aquatic stages of the Afrotropical malaria vector mosquito Anopheles gambiae in the laboratory

Examination of heat shock gene expression in nerve cord isolated from heat-stressed American cockroach, Periplaneta americana

Heat shock proteins and thermotolerance in a cultured cell line from the Mediterranean fruit fly, Ceratitis capitata

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