Heat-shock response in a tropical Chironomus: seasonal variation in response and the effect of developmental stage and tissue type on heat shock protein synthesis

Abstract

NATH, B. B., and LAKHOTIA, S. C. 1989. Heat-shock response in a tropical Chironomus: seasonal variation in response and the effect of developmental stage and tissue type on heat shock protein synthesis. Genome, 32: 676-686. Examination of heat shock induced transcriptional activity in salivary gland polytene nuclei of a tropical Chironomus, C. striatipennis, revealed nine heat-shock puffs. In 24°C-reared larvae optimal heat-shock response was seen at 39"C, while a 41°C shock was nearly lethal. In a population grown under natural conditions of seasonal variations, the heat-shock response was dependent upon the current ambient temperature. In summer months, response to 39°C was variable, from complete to no induction of heat-shock puffs in different cells. In control glands from larvae growing at 33 -36°C in summer, heat-shock genes were not active, although in 24°C-reared larvae, 33°C already caused partial induction. Unlike the 24°C-reared population, a 41 "C shock to summer larvae was not lethal.[35S]Methionine-labelled protein synthesis pattern in the summer larvae revealed appreciable accumulation of heat-shock polypeptides in control glands, which possibly autoregulates their further induction and also explains the better thermotolerance of these larvae. In a developmental study of a 24°C-reared population, some heat-shock polypeptides were found to be commonly synthesized at 39°C in all the tissues (salivary glands of larvae; Malpighian tubules of larvae, pupae, and adult; adult ovaries), while other heat-shock polypeptides showed apparent tissue and (or) developmental stage specificity. Heat shock protein 70 was most abundantly synthesized in all the tissues examined.NATH, B. B., et LAKHOTIA, S. C. 1989. Heat-shock response in a tropical Chironomus: seasonal variation in response and the effect of developmental stage and tissue type on heat shock protein synthesis. Genome, 32 : 676-686. L'examen des activitks transcriptionnelles induites par des chocs thermiques dans les noyaux de la glande salivaire d'un Chironomus, le C. striatipennis, a rkvklk la prksence de neuf renflements causes par des chocs thermiques. Chez des larves soumises 8 une tempkrature d'klevage de 24"C, la rkponse 8 une tempkrature choc optimale a kt6 vue 8 39"C, alors qu'une tempkrature de 41 "C s'est avkrke presque lktale. Chez une population croissant dans des conditions naturelles de variations -saisonni&res, la rkponse aux chocs therrniques a varik selon les tempkratures ambiantes. En ktk, la rkponse 2 une tempkrature de 39°C a varik depuis l'absence d'induction 8 une induction complkte de renflements dans diffkrentes cellules. Dans les glandes tkmoins de larves croissant 8 33-36°C en ktk, les g&nes de chocs thermiques n'ont pas kt6 actifs tandis que chez les larves klevkes 8 24"C, une tempkrature de 33°C causait dkj8 une induction partielle. Contrairement aux populations klevks 8 24"C, la tempkrature de 41 "C ne s'est pas avkrke lktale pour les larves d'ktk. Le profil de synth&se de protkines marqukes 8 la mkthionine [S35] chez les l...

A novel computational approach of image analysis to quantify behavioural response to heat shock in Chironomus Ramosus larvae (Diptera: Chironomidae)

“…Above 40 °C, the larvae were found to come out from the "housing tube" and showed aberrant swimming behaviour. Th ese fi ndings corroborated with the result reported in another tropical species of midge C. striatipennis, where 39 °C was found to be the optimal threshold temperature for cytogenetic manifestation of heat shock response and above 39 °C, cell death was observed in laboratory reared larvae as evidenced by pycnotic nuclei (Nath and Lakhotia 1989). Our fi ndings suggest that the threshold temperature for sensing the external temperature stress might be the same both at the cellular and at the whole organismal level.…”

“…Chironomid midges show adaptations to a wide range of temperature fluctuations (Oliver 1971;Ferrington 2008) and appropriately qualify as models to study temperature stress. Previously, several works on HSPs and heat shock inducible chromosomal loci were reported mainly in two European species, namely, Chironomus tentans Fabricius and Chironomus thummi (Kieffer) (Tanguay and Vincent 1981;Lezzi 1984;Carretero et al 1991;Sass 1995;Martinez et al 1997;Morcillo et al 1997) and in one Indian species, Chironomus striatipennis Kieffer (Nath and Lakhotia 1989). In the present study, a tropical Indian species, Chironomus ramosus Chaudhuri, Das and Sublette, colonized in the laboratory (Nath and Godbole 1998) has been chosen to study behavioural response to heat shock.…”

Chromosomal Response to Chemical Stress in Flesh Fly Sarcophaga ruficornis (Fab.) (Sarcophagidae: Diptera)

“…It is possible that the activity at this locus mediates the activity of other loci. In response to heat shock and other stresses, several puffs are induced in Bactrocera (Mavaragani-Tsipidou 2002), Ceratitis (Chrysanthakopoulou et al 1998, Papadimitriou et al 1998, Chironomus (Yamamoto 1970, Vincent and Tanguay 1979, Lezzi et al 1981, Morcillo et al 1982, Lezzi 1984, Nath and Lakhotia 1989, Drosophila (Ritossa 1964, Ashburner 1970, Ashburner and Bonner 1979, Lakhotia and Singh 1982, 1985, Lakhotia and Prasanth 2002, Lucilia (Joshi and Tiwari 2000), Musca (el Agoze 1993) and Rhynchosciara (Stocker et al 2006).…”

“…Besides Drosophila, cytological studies on the stress response have so far been limited only to a few other dipterans, namely, Chironomus (Yamamoto 1970, Vincent and Tanguay 1979, Lezzi 1984, Lezzi et al 1981, Nath and Lakhotia 1989, Morcillo et al 1982, 1994, Sass 1995, Sarcophaga (Bultmann 1986a, b), Parasarcophaga (Ranjan and Kaul 1988), Ceratitis (Papadimitriou et al 1998, Chrysanthakopoulou et al 1998, Lucilia (Joshi and Tiwari 2000), Bactrocera (Zambetaki et al 2000, Mavragani-Tsipidou 2002 and Rhynchosciara (Stocker et al 2006). Therefore, it is imperative that stress response is observed in other dipterans also with a view to understand the adaptive significance of stress response in relation to the organism diversity.…”

Chironomus strenzkei Fittkau, 1968 is a junior synonym of C. striatipennis Kieffer, 1910

“…These have been used for morphological (e.g. Chaudhuri et al 1992;Amora et al 2015, Martin 2017, and molecular analysis, with some limited cytological studies (Nath and Lakhotia 1989, and Gupta and Kumar 1991, Martin 2017. Those used for molecular analysis are listed in Pramual et al (2016) and confirmed the conclusion from morphological and cytological studies, that C. kiiensis (group B of Kondo et al 2016) was a junior synonym of C. striatipennis.…”