Heat Shock Proteins in the Pathogenesis of Mycobacterium tuberculosis

Tripathi, Prajna; Batra, Janendra K.

doi:10.1007/978-981-32-9413-4_13

Cited by 1 publication

(1 citation statement)

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“…Among transcriptome differences between MP and NP mycobacteria, it is worth mentioning prominent up-regulation of several DosR regulon genes, including their regulator DosR, and genes for GrpE/GroEL2 chaperons and their transcriptional regulator HspR in the NP group. This pattern is consistent with reduced metabolic activity of mycobacteria and transition to dormancy [48,53].…”

Section: Mycobacterial Genes Differentially Changing the Expression W...supporting

confidence: 77%

An In Vivo Model of Separate M. tuberculosis Phagocytosis by Neutrophils and Macrophages: Gene Expression Profiles in the Parasite and Disease Development in the Mouse Host

Kondratieva

Majorov

Grigorov

et al. 2022

IJMS

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The role of neutrophils in tuberculosis infection remains less well studied compared to that of the CD4+ T-lymphocytes and macrophages. Thus, alterations in Mycobacterium tuberculosis transcription profile following phagocytosis by neutrophils and how these shifts differ from those caused by macrophage phagocytosis remain unknown. We developed a mouse model that allows obtaining large amounts of either neutrophils or macrophages infected in vivo with M. tuberculosis for mycobacteria isolation in quantities sufficient for the whole genome RNA sequencing and aerosol challenge of mice. Here, we present: (i) the differences in transcription profiles of mycobacteria isolated from liquid cultures, neutrophils and macrophages infected in vivo; (ii) phenotypes of infection and lung inflammation (life span, colony forming units (CFU) counts in organs, lung pathology, immune cells infiltration and cytokine production) in genetically TB-susceptible mice identically infected via respiratory tract with neutrophil-passaged (NP), macrophage-passaged (MP) and conventionally prepared (CP) mycobacteria. Two-hour residence within neutrophils caused transcriptome shifts consistent with mycobacterial transition to dormancy and diminished their capacity to attract immune cells to infected lung tissue. Mycobacterial multiplication in organs did not depend upon pre-phagocytosis, whilst survival time of infected mice was shorter in the group infected with NP bacilli. We also discuss possible reasons for these phenotypic divergences.

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Section: Mycobacterial Genes Differentially Changing the Expression W...supporting

confidence: 77%